Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines

Aliyazıcıoğlu

El-Cezeri Fen Ve Mühendislik Dergisi

2017

Self Cite

Laurocerasus officinalis Roem. Prunus cinsine ve Rosaceae familyasına mensup bir tür olup, Türkiye'de halk arasında "karayemiş" veya "taflan" adı ile bilinmektedir. Diyetsel fenolik bileşikler doğal antioksidan maddeler olup, bitkisel kaynaklarda aglikon halde bulunabildikleri gibi glikozile halde de bulunabilmektedirler. Genel kanı antioksidan aktivitelerinin hidroksil grubu sayısıyla orantılı olarak arttığı ve glikozilasyon derecesi ile orantılı şekilde azaldığı yönündedir. Çeşitli çalışmalarda uygulanan asidifikasyon işleminin doğal ürünlerden elde edilen ekstraktların antioksidan gücünü asidifikasyon uygulanmayan ham ekstraktlara göre arttırdığı gösterilmiştir. Bu çalışmanın amacı farklı çözücülerle hazırlanmış L. officinalis ekstraktlarının asidifikasyon işlemi sonucu antioksidan özelliklerindeki değişikliklerin belirlenmesidir. Çalışmada L. officinalis'in etanol, dimetil sülfoksit (DMSO) ve bu çözücülerin % 0.5 (v/v) oranında hidroklorik asit içeren formları ile dört farklı ekstrakt hazırlandı. Ekstraktların antioksidan özellikleri toplam fenolik madde tayini, toplam flavonoid madde tayini, demir indirgeyici güç tayini ve 2,2-difenil-1-pikrilhidrazil (DPPH) radikal temizleme aktivitesi metotları kullanılarak belirlendi. DMSO ile hazırlanan ekstraktın antioksidan aktivite değerleri etanollü ekstrakta göre istatistiksel olarak anlamlı derecede yüksek bulundu (p<0.05). Asidifikasyon işleminin her iki çözücüyle de hazırlanan ekstraktların antioksidan özelliklerini istatistiksel olarak anlamlı derecede arttırdığı gözlendi (p<0.05). Asidifikasyon işlemi sonucu antioksidan aktivite değerlerindeki yükselmenin biyolojik aktivitelere ne oranda yansıdığının gösterilmesi için daha kapsamlı çalışmalar gerekmektedir.

Section: Bulgular Ve Tartışmaunclassified

Section: Introductionunclassified

Asidifikasyon İşleminin Laurocerasus officinalis Ekstraktlarının Antioksidan Özellikleri Üzerine Etkisinin İncelenmesi

Aliyazıcıoğlu

El-Cezeri Fen Ve Mühendislik Dergisi

2017

Self Cite

Acta Sci.Pol.Hortorum Cultus

“…Therefore, they are commonly used for diabetes, tissue and dermatological disorders. Fruits are also known to be quite effective in cell renewal and against cancer cells [İslam 2004, Karahalil and Şahin 2011, Yıldız et al 2014, Demir et al 2017.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies were performed on cherry laurel for selection of superior genotypes [İslam andOdabaş 1996, İslam et al 2010], propagation techniques were developed to widespread promising genotypes [Sülüşoğlu and Çavuşoğlu 2009]; studies were also performed about consumption method and types to increase consumptions [Temiz et al 2014], about nutritional composition to put forth the health effects of the fruits [Kalyoncu et al 2013, Yıldız et al 2014, Demir et al 2017.…”

Section: Introductionmentioning

confidence: 99%

BIOACTIVE COMPOUNDS AND PHYSICO-MECHANICAL ATTRIBUTES OF FRUIT AND STONE OF CHERRY LAUREL (Prunus laurocerasus) HARVESTED AT DIFFERENT MATURITY STAGES

Altuntaş¹,

Öztürk²,

Kalyoncu³

2018

In the study, physico-mechanical properties [geometric mean diameter (D g), sphericity, surface area, weight (W), bulk and fruit density, color, fruit removal force (FRF), puncture and compression force, puncture and compression deformation, friction coefficient] and biochemical attributes [pH, soluble solids content (SSC), titratable acidity (TA), vitamin C, total phenolics (TPs), total monomeric anthocyanin (TMA) and total antioxidant capacity (TAC)] of cherry laurel fruits (Prunus laurocerasus) and stones were investigated. Geometric mean diameter, surface area, fruit weight, bulk and fruit density significantly increased, but L*, a* and b* values significantly decreased with the progress of ripening. Fruit removal force decreased from 5.04 N to 3.09 N. On the other hand, W/FRF increased with the progress of harvest date. Static friction coefficient of fruits significantly increased over plywood and silicone surfaces and friction coefficients of stones increased over plywood, galvanized mild steel and silicone surfaces with the progress of harvest date. The pH, SSC, vitamin C, TPs, TMA and TAC (according to FRAP and TEAC assay) values significantly increased with the progress of ripening. It was concluded that fruit maturity stage at harvest had significant impact on physico-mechanical attributes and bioactive compounds of cherry laurel fruits.

“…Zhang et al (2013) reported that ethyl acetate and butanol fractions of aqueous extract of Peltophorum pterocarpum exhibits cytotoxic effect on human leukemia (HL-60), and stomach cancer (AZ521) cells, while Hussein et al (2016) demonstrated that Caesalpinia ferrea Martius extract exhibits a cytotoxic effect on human liver (HepG2), breast (MCF-7), colon (HCT-116), larynx (Hep2) and prostate (PC-3) cancer cells. Recently, Bencherchar et al (2017) reported that chloroform, ethyl acetate, and methanolic extract of Genista ferox exhibits a cytotoxic effect on HeLa cells.Polyphenols are an important class of secondary plant metabolites and are known to have strong antioxidant character(Turan et al, 2015;Demir et al, 2017b). These strong antioxidant properties of phenolic compounds are reported to provide cytotoxic, anticarcinogenic, antidiabetic, antibacterial and anti-…”

mentioning

confidence: 99%

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Aliyazıcıoğlu

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

Yaman

et al. 2019

The purpose of this study was to investigate the phytochemical analysis of D. pentaphyllum and its cytotoxic activity on human cervical cancer (HeLa) cells with possible mechanisms. Total phenolic contents (TPC) and phytochemical analysis of the extract were evaluated using spectrophotometric method and RP-HPLC, respectively. The cytotoxic effect of the extract was evaluated using the MTT assay. The mechanism of the cytotoxic effect of the extract was also evaluated in terms of apoptosis, cell cycle, and mitochondrial membrane potential (MMP) using the fluorometric methods. The TPC of the extract was calculated as 68.9±2.3 mg gallic acid equivalent per g sample and quercetin was determined as the major phenolic in the extract. The extract showed a selective cytotoxic effect (6.5-fold) on HeLa cells compared to normal fibroblast cells. The cytotoxic effect of the extract was found to be due to the arresting of the cell cycle in the S phase and increasing apoptosis through decreased MMP. Further studies are needed for the comprehensive effects of the extract on cell signaling.