Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Aliyazıcıoğlu, Yüksel; Demir, Selim; Yaman, Serap Özer; Şener, Sila; Demir, Elif; Aliyazıcıoğlu, Rezzan; Turan, İbrahim

doi:10.18016/ksutarimdoga.vi.579938

Cited by 10 publications

(1 citation statement)

References 36 publications

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“…ROS formation induced by 5-FU increases lipid peroxidation (15). It is known that increased ROS causes protein and DNA damage, especially lipids in cells (22)(23)(24). MDA is considered the most important indicator of lipid peroxidation level (25).…”

Section: Discussionmentioning

confidence: 99%

N-Acetylcysteine Ameliorates 5-Fluorouracil‐Induced Ovarian Injury in Rats

AYAZOGLU DEMİR,

MENTESE,

KÜÇÜK

et al. 2023

Clinical and Experimental Health Sciences

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Objective: Although 5-fluorouracil (5-FU) is one of the most commonly used chemotherapeutics worldwide, it has been shown that 5-FU administration can cause reproductive toxicity in recent years. N-acetylcysteine (NAC) is the precursor of glutathione, the most important endogenous antioxidant molecule and is known for its effective antioxidant and anti-inflammatory properties. Although NAC is one of the most studied antioxidant molecules, its curative effect against ovarian damage caused by 5-FU has not been demonstrated to date. It was therefore aimed to investigate whether NAC is therapeutic against 5-FU-induced ovotoxicity in this study for the first time. Methods: Rats were first exposed to a single dose of 5-FU (100 mg/kg) and then treated with NAC (10 and 20 mg/kg) for three days. The oxidative stress, inflammation and apoptosis markers in ovarian tissues were also determined using spectrophotometric methods. Ovarian tissues were also evaluated histologically. Results: It was revealed that the levels of oxidative stress, inflammation and apoptosis biomarkers in ovarian tissue increased by 5-FU administration (p

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Section: Discussionmentioning

confidence: 99%

N-Acetylcysteine Ameliorates 5-Fluorouracil‐Induced Ovarian Injury in Rats

AYAZOGLU DEMİR,

MENTESE,

KÜÇÜK

et al. 2023

Clinical and Experimental Health Sciences

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The therapeutic effect of silibinin against 5‐fluorouracil‐induced ovarian toxicity in rats

Demir

Menteşe

Kucuk

et al. 2023

J Biochem & Molecular Tox

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Abstract5‐Fluorouracil (5‐FU) is a fluoropyrimidine group antineoplastic drug with antimetabolite properties and ovotoxicity is one of the most important side effects. Silibinin (SLB) is a natural compound that is used worldwide and stands out with its antioxidant and anti‐inflammatory properties. The aim of this study was to evaluate the therapeutic effect of SLB in 5‐FU‐induced ovototoxicity using biochemical and histological analysis. This study was carried out in five main groups containing six rats in each group: control, SLB (5 mg/kg), 5‐FU (100 mg/kg), 5‐FU + SLB (2.5 mg/kg), and 5‐FU + SLB (5 mg/kg). The levels of ovarian malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT), 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), tumor necrosis factor‐alpha (TNF‐α), myeloperoxidase (MPO), and caspase‐3 were determined using spectrophotometric methods. Hematoxylin and eosin staining method was employed for histopathological examination. MDA, TOS, 8‐OHdG, TNF‐α, MPO, and caspase‐3 levels in 5‐FU group were significantly increased compared with the control group, while the levels of TAS, SOD, and CAT were decreased (p < 0.05). SLB treatments statistically significantly restored this damage in a dose‐dependent manner (p < 0.05). Although vascular congestion, edema, hemorrhage, follicular degeneration, and leukocyte infiltration were significantly higher in the 5‐FU group compared with the control group, SLB treatments also statistically significantly restored these damages (p < 0.05). In conclusion, SLB has a therapeutic effect on the ovarian damage induced by 5‐FU via decreasing the levels of oxidative stress, inflammation, and apoptosis. It may be helpful to consider the usefulness of SLB as an adjuvant therapy to counteract the side effects of chemotherapy.

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