CYTOTOXIC AND ANTIBACTERIAL ACTIVITIES OF MARINE SPONGE-DERIVED FUNGUS Aspergillus nomius NC06

Artasasta, Muh. Ade; Yanwirasti,; Bakhtiar, M. Taher; Djamaan, Akmal; Handayani, Dian

doi:10.31788/rjc.2019.1235284

Cited by 8 publications

(12 citation statements)

References 15 publications

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“…The lowest concentration contained a clear zone minimum inhibitory concentration (MIC) value. 18 . (2018).…”

Section: Resultsmentioning

confidence: 99%

BIOACTIVE METABOLITE FROM MARINE SPONGEDERIVED FUNGUS Cochliobolus geniculatus WR12

Handayani¹,

Putri²,

Ismed³

et al. 2020

RJC

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This study aims to find bioactive metabolites from fungus, which was isolated from marine sponge Haliclona fascigera. The fungus was identified molecularly as C. geniculatus WR12. The isolation of the bioactive compound was achieved by column chromatography with step gradient polarity (SGP) method and purification by recrystallization. The structure was determined based on spectroscopic analyses (UV, IR, HR-MS, 1 H and 13 C NMR). The antibacterial activity of the compound was tested against Methicillin-Resistant Staphylococcus Aureus (MRSA) by using the agar diffusion method. The Cytotoxicity of the compound was tested on the cancer cell lines by using the microculture tetrazolium (MTT) assay. The results of this study were obtained a pure compound (1). The compound was identified as radicinin based on 1D and 2D NMR and HRESIMS data. This compound showed cytotoxic activity with IC 50 values of 60.68, 30.89, and 87.89 µg/mL against WiDr, T47D, and Hela cell lines, respectively, but not toxic against Vero cell (IC 50 value of 607.31 µg/mL). Radicinin showed higher cytotoxic activity against T47D cells (IC 50 = 25.01 ppm) compared with doxorubicin (IC 50 = 33.49 ppm). The result of the antibacterial activity of radicinin showed the minimum inhibitory concentration (MIC) of 125 µg/disc against MRSA. Based on these results, it can be concluded that radicinin could be considered as a potential candidate for future anticancer and anti-MRSA drug.

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“…The lowest concentration contained a clear zone minimum inhibitory concentration (MIC) value. 18 . (2018).…”

Section: Resultsmentioning

confidence: 99%

BIOACTIVE METABOLITE FROM MARINE SPONGEDERIVED FUNGUS Cochliobolus geniculatus WR12

Handayani¹,

Putri²,

Ismed³

et al. 2020

RJC

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“…17 This compound has also been isolated from Phomopsis sp IFB-E060, an endophytic fungus on the bark of Vaticamangachapoi 18 , from Phomopsis sp By254, an endophytic fungus derived from Gossypium hirsutum root 19 , and from Phomopsis sp ZZF08, the endophytic fungus from the mangrove bark, which was collected in Zhanjiang, South China Sea. 11 This is the first report on the isolation of cytochalasin H from the endophytic fungus D. amygdali. To date, more than 300 cytochalasin analog compounds have been isolated from various species of fungi.…”

Section: Characterization Of Pure Compoundmentioning

confidence: 95%

“…Antibacterial activity was detected after incubation for 24 h at 30 o C. A clear zone of inhibition in the media was considered as an indicator of antibacterial activity. 11,12,13…”

Section: Antibacterial Activity Testingmentioning

confidence: 99%

BIOACTIVE COMPOUND FROM THE MANGROVE PLANT ENDOPHYTIC FUNGUS Diaporthe amygdali SgKB4

Handayani¹,

Wahyuningsih²,

Rustini³

et al. 2020

RJC

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Investigation of the chemical constituent and antibacterial activity of Diaporthe amygdali SgKB4, an endophytic fungal strain isolated from the West Sumatran mangrove plant Sonneratiagriffithii Kurz led to the isolation of compound 1. The isolation of compound 1 was achieved by column chromatography with step gradient polarity (SGP) method and purification by recrystallization. The structure of compound 1 was elucidated based on nuclear magnetic resonance (NMR) spectroscopy data. Based on IR, 1D, and 2D-NMR, as well as MS data, the isolated compound is cytochalasin H. This compound showed mild antibacterial activity against some pathogenic bacteria. This is the first report on the isolation of cytochalasin H from the endophytic fungus D.amygdali.

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“…Jamur endofit yang berasal dari laut hidup di jaringan internal tanpa menimbulkan efek negatif pada inangnya telah terbukti menjadi sumber produk alami bioaktif yang produktif dengan struktur unik dan aktivitas farmasi yang kuat (Deshmukh et al, 2018;Artasasta et al, 2019;Mongi et al, 2020;Sumilat et al, 2020). Jamur dapat ditemukan di hampir setiap habitat laut (Raghukumar, 2017), termasuk tanaman laut (alga, kayu apung, dan tanaman bakau), avertebrata laut (spons, karang, ascidia, dan holothuria), dan vertebrata (terutama ikan).…”

Section: Pendahuluanunclassified

“…Berdasarkan skrining awal yang telah dilakukan, A. nomius juga merupakan spesies jamur yang memiliki potensi antimikroba. Artasasta et al, (2019) (Butanol, Kloroform, Etil Asetat). Dari beberapa hasil penelitian di atas terhadap jamur A. nomius, ditemukan jamur ini memiliki aktivitas antibakteri yang kuat seperti skrining awal dari Strain MFALM 2 yang memperlihatkan kemampuan menghambat pertumbuhan bakteri S. aureus dan E. coli.…”

Section: Identifikasi Molekuler Dnaunclassified

Antibacterial potential of marine fungus Aspergillus nomius isolated from green algae Bornetella sp

Sumilat

Lintang

2021

JIP

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Isolation of marine fungi symbiont of green algae Bornetella sp as a producer of antibacterial compounds has been carried out. This study aims to obtain symbiont fungi from green algae Bornetella sp which produces antibacterial compounds. The symbiont fungus was isolated using the direct planting method. Screening for the antibacterial activity of pure symbiont fungi isolates against Escheriscia coli and Staphylococcus aureus, and was carried out by the agar diffusion method. The isolation results obtained 4 isolates, but the one with the most potential to inhibit the growth of the tested bacteria was MFALM2. Molecular characterization showed that the MFALM2 isolate was identified as Aspergillus nomius with a 100% closeness level. Keywords: isolation; marine fungi; Aspergillus nomius; green alga; Bornetella sp. AbstrakIsolasi jamur laut yang bersimbion dengan alga hijau Bornetella sp sebagai penghasil senyawa antibakteri telah dilakukan. Penelitian ini bertujuan untuk memperoleh jamur simbion dari alga hijau Bornetella sp yang menghasilkan senyawa antibakteri. Isolasi jamur simbion dilakukan dengan metode direct planting. Skrining aktivitas antibakteri isolat murni jamur simbion terhadap bakteri uji Escheriscia coli dan Staphylococcus aureus dilakukan dengan metode difusi agar. Hasil isolasi diperoleh 4 isolat, namun yang paling berpotensi menghambat pertumbuhan bakteri uji adalah MFALM2. Karakterisasi molekuler menunjukkan bahwa isolat MFALM2 teridentifikasi sebagai Aspergillus nomius dengan tingkat keeratan sebesar 100%.Kata kunci: isolasi; jamur lau; Aspergillus nomius; alga hijau; Bornetella sp.

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CYTOTOXIC AND ANTIBACTERIAL ACTIVITIES OF MARINE SPONGE-DERIVED FUNGUS Aspergillus nomius NC06

Cited by 8 publications

References 15 publications

BIOACTIVE METABOLITE FROM MARINE SPONGEDERIVED FUNGUS Cochliobolus geniculatus WR12

BIOACTIVE METABOLITE FROM MARINE SPONGEDERIVED FUNGUS Cochliobolus geniculatus WR12

BIOACTIVE COMPOUND FROM THE MANGROVE PLANT ENDOPHYTIC FUNGUS Diaporthe amygdali SgKB4

Antibacterial potential of marine fungus Aspergillus nomius isolated from green algae Bornetella sp

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