Cytotoxic Activity of Stem Bark Extracts from Canarium odontophyllum Miq (Dabai) against Human Colorectal Carcinoma HCT 116 Cell Line

Basri, Dayang Fredalina; Mohd, Muhammad Arifuddin Al Rasyid; Meng, Cuida; Latif, Elda Surhaida; Huyop, Fahrul

doi:10.4236/ajps.2014.526411

Cited by 4 publications

(9 citation statements)

References 18 publications

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“…Out of all three extracts, acetone extract exhibited the most potent cytotoxic activity with a significantly higher cytotoxic effect than aqueous extract but the cytotoxicity between acetone and methanol extract showed no significant difference. This finding correlates with the previous study which reported that acetone extract from the stem bark of C. odontophyllum [ 12 ] displayed the highest cytotoxic effect against HCT 116 cells and was also in agreement with acetone extract from the leaves of C. odontophyllum [ 11 ]. The cytotoxic activity of a plant against cancer cells is based on their phytochemical properties [ 22 ].…”

Section: Discussionsupporting

confidence: 92%

“…The cytotoxic activity of a plant against cancer cells is based on their phytochemical properties [ 22 ]. The phytochemical screening of aqueous, methanol and acetone extracts from stem bark of C. odontophyllum showed that all three extracts contain flavonoid, saponin, tannin, terpenoid and phenolic compounds [ 12 ]. The potent cytotoxic effect exerted by acetone extract may be caused by non-polar terpenoid compound.…”

Section: Discussionmentioning

confidence: 99%

“…The leaf and shell extracts from C. odontophyllum were shown to have antimicrobial activity against a wide range of pathogenic bacteria [ 10 ] whereas both the leaf and stem bark of C. odontophyllum demonstrated promising anticancer property [ 11 ]. However, previous study merely reported preliminary screening of cytotoxic activity against human colorectal carcinoma HCT 116 cell line attributed to the presence of flavonoid, tannin, saponin, terpenoid and phenolic compound [ 12 ]. Damage to DNA always occurs from endogenous and exogenous agents such as reactive oxygen species (ROS) from cellular metabolism and ultraviolet light from the sun [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Basri¹,

Alamin²,

Chan³

2015

BMC Complement Altern Med

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Background Canarium odontophyllum Miq. is a plant species widely known as ‘dabai’ and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line.MethodThe IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200 μg/ml at treatment time of 24, 48 and 72 h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48 h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30 min of IC10 and IC25 treatment.ResultsAcetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24, 48 and 72 h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48 h, however at 72 h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48 h treatment. Low doses of acetone extract from stem bark of C. odontophyllum showed significant DNA damage in HCT 116 cells with tail moment of 6.187 ± 0.718 A.U and 7.877 ± 0.142 A.U, respectively.ConclusionsAcetone extract from stem bark of C. odontophyllum has high potential in the development of anticancer agent against HCT 116 cells with no cytotoxic effect against human colon fibroblast cells.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Basri¹,

Alamin²,

Chan³

2015

BMC Complement Altern Med

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“… 9 The stem bark of this tree is gray or yellowish from the outside while the inside is pink with a soft texture and produces a fragrant aroma. 10 , 11 Typically, the harvesting season runs from November until January. Because of the plant’s rapid respiration rate, its fruits have a short shelf life.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative Activities of Acetone Extract From Canarium Odontophyllum (Dabai) Stem Bark Against Human Colorectal Cancer Cells

2023

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Colorectal cancer is the most common malignant cancer in developing countries. Canarium odontophyllum, also known as “Dabai” or “Borneo Olive“ is among the natural plants that can potentially be used as an anticancer agent. This study aims to determine the antiproliferative activities and cytotoxicity effects of acetone extract from C. odontophyllum stem bark against human colorectal cancer cell lines HCT 116 and HT 29. Acetone extract of C. odontophyllum stem bark exerted a significant cytotoxic effect on HCT 116 and HT 29 cells determined by MTT assay at the concentration of 12.5 μg/mL to 200 μg/mL for 24, 48, and 72 hours treatment. It was found that acetone extract of C. odontophyllum stem bark inhibited proliferation of HCT 116 with an IC50 value of 184.93 ± .0 μg/mL, 61.24 ± .1 μg/mL, 79.98 ± .029 for 24, 48 and 72 hours respectively. The findings also showed that acetone extract of C. odontophyllum stem bark revealed a lower inhibitory effect against HT-29 with an IC50 value of more than 200 μg/mL for 24, 48 and 72 hours. However, acetone extract of C. odontophyllum stem bark at similar concentrations and time points did not show any cytotoxic effect to normal colorectal fibroblast cell CCD18-Co. In conclusion, the acetone extract of C. odontophyllum stem bark exhibited more sensitivity against HCT 116 than HT 29. Its antiproliferative ability towards HCT 116 and HT 29 cells provides insight that this extract may serve as an anticancer agent against colorectal cancer.

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“…The aqueous extract has been shown to reduce sugar level in diabetic rat (Saari et al, 2017). Apart from its leaf, study on stem bark and fruit of Canarium odontophyllum tree has also been conducted and have been proven as anti-oxidant (Shakirin et al, 2010), anti-cancer agent (Basri et al, 2014a), anti-atherosclerotic (Shakirin et al, 2012), anti-microbial and anti-yeast (Basri et al, 2014b). However, studies on Canarium odontophyllum leave against Leptospira bacteria have not been conducted yet.…”

Section: Introductionmentioning

confidence: 99%

In-vitro antileptospiral activity of Canarium odontophyllum Miq. (Dabai) leaves extract

Ishak¹,

Ariffudin

Azmi

et al. 2019

MJM

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Aims: To evaluate the anti-leptospiral activity of Canarium odontophyllum leaves against Leptospira interrogans serovar Bataviae and Leptospira borgpetersenii serovar Javanica. Methodology and results: The extracts (hexane, acetone, methanol and aqueous) used in this study were tested at concentration ranging from 0.049 mg/mL to 25 mg/mL using broth microdilution method. Percentage inhibition (%) was obtained through OD reading at 400 nm. Only methanol extract was incubated with Leptospira to observe population changes under dark field microscope prior to subjected for DNA damaging studies through gel electrophoresis of genomic DNA. Methanol extract showed the highest percentage inhibition of 66% against L.interrogans serovar Bataviae and 74% against L. borgpetersenii serovar Javanica. The IC50 value of methanol extract was 4.60 mg/mL and 2.25 mg/mL against serovar Bataviae and serovar Javanica, respectively. Both Leptospira culture which was treated with IC50 value of methanol extract showed drastic decrease in population compared to untreated Leptospira for both serovar. There was no DNA damage towards serovar Bataviae. However, serovar Javanica exhibited DNA damage as observed from the presence of DNA fragmentation on the gel electrophoresis. Conclusion, significance and impact of study: These findings confirmed that methanol leaves extract from of Canarium odontophyllum has a potential to control leptospirosis.

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Cytotoxic Activity of Stem Bark Extracts from Canarium odontophyllum Miq (Dabai) against Human Colorectal Carcinoma HCT 116 Cell Line

Cited by 4 publications

References 18 publications

Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Antiproliferative Activities of Acetone Extract From Canarium Odontophyllum (Dabai) Stem Bark Against Human Colorectal Cancer Cells

In-vitro antileptospiral activity of Canarium odontophyllum Miq. (Dabai) leaves extract

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