Cytotoxic Activity of Extract and Active Fraction of Turbinaria decurrens Bory on Colon Cancer Cell Line HCT-116

Deviyani-Zakaria, Astria; Basah, Katrin; Bahtiar, Anton

doi:10.4067/s0717-95022018000300979

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…The methanolic extracts of A. platensis showed varying degrees of inhibitory efficacy against the four evaluated human tumor cell lines. This agreed with Mashjoor et al [ 35 ], Hernandez et al [ 36 ] and Deviyani et al [ 37 ] who reported cytotoxic potential and activity of the A. platensis extracts against three cell lines, including MCF7, HeLa and HepG2.…”

Section: Discussionsupporting

confidence: 91%

Potential Activity of Arthrospira platensis as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application

et al. 2023

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This research evaluated the antifungal effectiveness of Arthrospira platensis ethanol, methanol, ethyl acetate and acetone extracts against the tested pathogenic fungi (Candida albicans, Trichophyton rubrum and Malassezia furfur). Antioxidant and cytotoxicity effectiveness of A. platensis extracts against four distinct cell lines were also assessed. Methanol extract of A. platensis exhibited the highest inhibition zones against Candida albicans as measured by the well diffusion method. A transmission electron micrograph of the treated group of Candida cells with A. platensis methanolic extract showed mild lysis and vacuolation of the cytoplasmic organelles. In vivo, after induced infection of mice by C. albicans and treatment with A. platensis methanolic extract cream, the skin layer emerged with the removal of Candida spherical plastopores. The extract of A. platensis recorded the highest antioxidant activity using the DPPH (2, 2- diphenyl-1-picrylhydrazyl) scavenging method (IC50 28 mg/mL). A cytotoxicity test using a MTT assay showed that the A. platensis extract had strong cytotoxic activity against the HepG2 cell line (IC50 20.56 ± 1.7 μg/mL) and moderate cytotoxic activity against MCF7 and the Hela cell (IC50 27.99 ± 2.1 μg/mL). Gas Chromatography/Mass Spectroscopy (GC/MS) results revealed that the effective activity of A. platensis extract could be linked to a synergistic impact between their prominent composition as alkaloids, phytol, fatty acids hydrocarbons, phenolics and phthalates. A. platensis extract contains active metabolites that constitute a promising source of antifungal, antioxidant and anti-proliferative compounds for the pharmaceutical drug industry.

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Section: Discussionsupporting

confidence: 91%

Potential Activity of Arthrospira platensis as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application

et al. 2023

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“…The chemical constituents of the crude extracts of the tested seaweeds were analyzed using GC-MS, and the identi ed compounds with their molecular formula, peak area, and the chemical group were presented in Tables 4-8 Indonesia were 1.512 µg mL − 1 , and 3.058 µg mL − 1 , respectively. Differences in cytotoxic effect may be due to differences in seaweed species, collection site, extraction solvent, and assay methods (Zakaria et al, 2018).…”

Section: Gc-ms Analysismentioning

confidence: 99%

In Vitro Anticancer Activity of Five Marine Seaweeds Extract From Egypt Against Human Breast and Colon Cancer Cell Lines

Mofeed

Deyab

Sabry

et al. 2021

Preprint

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Ulva fasciata, U. lactuca, Amphiroa anceps, Corallina mediterranea, and Sargassum filipendula extract were screened for their in vitro anticancer activity against human breast adenocarcinoma cell line (MCF-7) and colorectal carcinoma cell line (HCT-116). Algal extracts exhibited significantly dose-dependent anticancer activity without significant cytotoxicity against normal human fibroblasts. The maximum inhibitory percentages against MCF-7 and HCT-116 cell lines were recorded by U. lactuca (88.5 ± 1.08 %) and A. anceps (86.1 ± 2.88 %) extracts. Algal extracts showed cytotoxic effect against MCF-7 cell line with fifty percent inhibitory concentration (IC50) values ranging from 3.54 ± 1.2 to 21.2 ± 1.1 µg mL-1. The seaweeds extract has a less cytotoxic effect against HCT-116. FTIR and GC-MS analyses of the extracts indicated that the anticancer potential of the tested seaweeds may be returned to the presence of various anticancer compounds such as palmitic acid, oleic acid, retinoic acid, dihydroactinidiolide, thiosemicarbazide, diisobutyl phthalate, and phytol. Thus, seaweed extracts may be a promising natural source of safe anticancer agents against human breast and colon cancers.

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“…Previous studies revealed several mechanisms of seaweed extracts that exert anticancer effects (Farooqi et al, 2012;Chantree et al, 2021). In CRC, there is evidence suggesting the anticancer activities of seaweed (Alves et al, 2018;Deviyani-Zakaria et al, 2018;Al-Aadily et al, 2022). Interestingly, there was a low risk of CRC development in East Asian people who usually consumed seaweed (Cotas et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Ethyl Acetate Extract of Halymenia durvillei Induced Apoptosis, Autophagy, and Cell Cycle Arrest in Colorectal Cancer Cells

Chantree

Martviset

Sornchuer

et al. 2023

Prev. Nutr. Food Sci.

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Colorectal cancer is one of the most death-dealing cancers. However, conventional cancer treatments still have side effects. Therefore, novel chemotherapeutic agents with less side effects are still in search. A marine red seaweed, Halymenia durvillei, is recently interested in its anticancer effects. This study investigated the anticancer effect of ethyl acetate extract of H. durvillei (HDEA) on HT-29 colorectal cancer cells in association with the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway. HDEA-treated HT-29 and OUMS-36 cells were used for cell viability tests by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay. The effects of HDEA on apoptosis and cell cycle were evaluated. The nuclear morphology and mitochondrial membrane potential (m) were observed by Hoechst 33342 and JC-1 staining, respectively. The gene expression of PI3K, AKT, and mTOR genes was evaluated using a real-time semiquantitative reverse transcription-polymerase chain reaction. The corresponding protein expressions were assessed by western blot analysis. The result revealed that the cell viability of treated HT-29 cells diminished while that of OUMS-36 cells was non-significant. By the down-regulation of cyclin-dependent kinase 4 and cyclin D1, HDEA-treated HT-29 cells were arrested in the G0/G1 phase. By the up-regulation of cleaved poly(adenosine diphosphate-ribose) polymerase, caspase-9, caspase-8, caspase-3, and Bax, HDEA-treated HT-29 cells underwent apoptosis, but suppressed Bcl-2, disrupted nuclear morphology and m. Furthermore, treated HT-29 cells underwent autophagy by up-regulation of light chain 3-II and beclin-1. Lastly, HDEA suppressed the expression of PI3K, AKT, and mTOR. Therefore, HDEA exerts anticancer effects against HT-29 cells, confirmed by apoptosis, autophagy, and cell cycle arrest induction via regulation of the PI3K/AKT/mTOR signaling pathway.

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Cytotoxic Activity of Extract and Active Fraction of Turbinaria decurrens Bory on Colon Cancer Cell Line HCT-116

Cited by 7 publications

References 11 publications

Potential Activity of Arthrospira platensis as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application

Potential Activity of Arthrospira platensis as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application

In Vitro Anticancer Activity of Five Marine Seaweeds Extract From Egypt Against Human Breast and Colon Cancer Cell Lines

Ethyl Acetate Extract of Halymenia durvillei Induced Apoptosis, Autophagy, and Cell Cycle Arrest in Colorectal Cancer Cells

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