Cytoprotective Activity and Anti-inflammatory Properties of <i>Artemisia nilagirica</i> (Clarke) Extracts-A Study with Macrophages

Bhat, Mohd Yasin; Gul, Mir Zahoor; Khan, Nooruddin; Qureshi, Insaf Ahmed; Ghazi, Irfan Ahmad

doi:10.1080/22311866.2017.1334588

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…It should be emphasized that unexposed macrophages RAW 264.7 presented the highest levels of oxidative stress. It is consistent with Bhat et al’s (2017) conclusions that macrophages are very prone to oxidative stress. It is due to the expression of genes for antioxidant enzymes in the cells which help cells to ameliorate the damaged biochemical machinery within cells.…”

Section: Discussionsupporting

confidence: 92%

A comparative study of using free radical generators in the testing of chosen oxidative stress parameters in the different types of cells

Miranowicz-Dzierżawska

2018

Toxicol Ind Health

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The aim of this study was to assess whether there are differences between the results of determining oxidative stress markers obtained from different origin cell lines after exposure to chemicals generating free radicals. The studies considered two markers of oxidative stress: the level of thiobarbituric acid reactive substances (TBARS) and superoxide dismutase activity. The evaluation was performed in five cell lines: Chinese hamster ovary (CHO-9) cells, lung adenocarcinoma A549, macrophages RAW264.7, skin carcinoma cells A431, and keratinocytes HaCaT. Three compounds generating free radicals were used as a source of reactive oxygen/nitrogen: 2,2'-azobis-2-methyl-propanimidamide dihydrochloride (AAPH), sodium persulfate (SP), and 3-morpholinosydnonimine hydrochloride (SIN-1). The most appropriate cell line to assess the level of TBARS proved to be the murine macrophage cell line RAW 264.7. Equally, good performance was observed in the lung cancer cell line A549, but only when tested with AAPH and SP. In the case of measuring superoxide dismutase activity, it appeared that the most suitable cell line was also the RAW 264.7 line, although dispersion increased significantly at the highest concentrations of AAPH and SP measurements. When choosing a cell line to determine oxidative stress, the specificity of the stress-inducing compound and the parameter determined should be taken into consideration.

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Section: Discussionsupporting

confidence: 92%

A comparative study of using free radical generators in the testing of chosen oxidative stress parameters in the different types of cells

Miranowicz-Dzierżawska

2018

Toxicol Ind Health

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“…On the other hand, A. nilagirca is reported to have several important biological activities like antioxidant, cytoprotective, anti-inflammatory, antiproliferative, antibacterial, anticancer etc. [17][18][19][20][21][22][23][24][25]. Apart, this plant also possesses larvicidal, pupicidal, adulticidal and mosquito repellant activity [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

In vitro antimalarial activity evaluation of two ethnomedicinal plants against chloroquine sensitive and resistant strains of Plasmodium falciparum

Gogoi

Gogoi²,

Chetia

2021

Clin Phytosci

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Background In this study, we selected two medicinal plants Citrus maxima (Burm.) Merr. and Artemisia nilagirica (C.B. Clarke) Pamp. on the basis of their traditional use in the treatment of fever associated with malaria in Assam (India) and evaluated their antimalarial potential against Plasmodium falciparum strains. Methods The properly processed plant parts of C. maxima (Burm.) Merr. and A. nilagirica (C.B. Clarke) Pamp. were extracted with different solvents from nonpolar to polar by cold maceration technique. After that antimalarial activities of the extracts were evaluated against both chloroquine sensitive (3D7) and resistant (RKL-9) strains of P. falciparum using Giemsa staining light microscopy technique. The most active extract(s) was further screened for cytotoxicity potential against murine macrophage RAW264.7 cell line using MTT assay. Then preliminary phytochemical screening and qualitative fingerprint analysis of the active extract(s) were done to check the presence of different secondary metabolites. Results From the in vitro study, the hydro-alcoholic extract of C. maxima (Burm.) Merr. and methanol extract of A. nilagirica (C.B. Clarke) Pamp. were found to be the most active against both 3D7 and RKL-9 strains. In the cytotoxicity study, the CC50 values of the active extracts were found to be > 100 μg/ml, which suggested the safety of the extracts. Then phytochemical and fingerprint analysis revealed the presence of various important plant secondary metabolites in both the extracts. Conclusion The findings of this study confirmed the presence of antimalarial potential of hydro-alcoholic extract of C. maxima (Burm.) Merr. and methanol extract of A. nilagirica (C.B. Clarke) Pamp without having any toxic effect. Both the extracts showed IC50 values below 5 μg/ml against 3D7 and RKL-9 strains.

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“…Artemisia nilagirica has been known to be used in traditional and folk medicine in the form of decoctions and infusions prepared from almost all parts of the plant to treat nervous disorders, gastrointestinal problems, epilepsy, urinary problems, skin disorders and inflammatory problems [13,14] . In addition to this, there have been reports of other biological properties from this plant like antioxidant [15] , antibacterial [16] , cytoprotective [17] , anthelminthic, antifungal and antiulcer [13] . Essential oil obtained from this plant is also reported to have many medicinal applications [18] .…”

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confidence: 99%

In vitro Evaluation of Antiproliferative, Lipoxygenase and Xanthine Oxidase Inhibitory Activities of Artemisia nilagirica (C.B.Clarke) Pamp. Leaf Extracts

Bhat¹,

Gul²,

Husain³

et al. 2019

pharmaceutical-sciences

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Bhat et al.: Cytotoxicity and Inhibition of Proinflammatory Enzymes by Artemisia nilagiricaThe current study was performed to screen the extracts of Artemisia nilagirica (C.B. Clarke) Pamp. for antiproliferative and antiinflammatory properties. Antiproliferative activity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Antiinflammatory activity was evaluated in terms of lipoxygenase and xanthine oxidase inhibitory activities. Results indicated that out of the 3 extracts tested, methanol extract was found to inhibit Y79 (human retinoblastoma) and MCF-7 (human breast adenocarcinoma) cell lines. The same extract was also found to inhibit lipoxygenase and xanthine oxidase enzymes in a concentration-dependent manner with an IC 50 of 128.20±3.39 and 144.23±2.04 µg/ ml for lipoxygenase and xanthine oxidase, respectively. The present study highlighted the antiproliferative potential of the plant against cancer cell lines. The extracts inhibited lipoxygenase and xanthine oxidase enzymes. Therefore, this plant could be a valuable source for anticancer and antiinflammatory molecules.

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Cytoprotective Activity and Anti-inflammatory Properties of Artemisia nilagirica (Clarke) Extracts-A Study with Macrophages

Cited by 5 publications

References 28 publications

A comparative study of using free radical generators in the testing of chosen oxidative stress parameters in the different types of cells

A comparative study of using free radical generators in the testing of chosen oxidative stress parameters in the different types of cells

In vitro antimalarial activity evaluation of two ethnomedicinal plants against chloroquine sensitive and resistant strains of Plasmodium falciparum

In vitro Evaluation of Antiproliferative, Lipoxygenase and Xanthine Oxidase Inhibitory Activities of Artemisia nilagirica (C.B.Clarke) Pamp. Leaf Extracts

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