Cytoplasmic Oestradiol-Binding Sites and Their Relationship to Oestradiol Content in the Endometrium of Cattle

Senior, Boris

doi:10.1530/jrf.0.0440501

Cited by 19 publications

(6 citation statements)

References 26 publications

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“…The presence of a conceptus suppressed both oestro¬ gen receptor mRNA and protein in the endome¬ trium. In cyclic sheep (Koligian & Stormshak, 1977;Miller et al 1911b;Findlay et al 1982;Cherny et al 1991) and cattle (Kimball & Hansel, 1974;Senior, 1975;Zelinski et al 1980;Meyer et al 1988), oestro¬ gen receptor levels are greatest during oestrus and decline under the influence of chronically elevated progesterone during dioestrus. For endometrial oestrogen receptors, the relative amounts of mRNA and protein were approximately five-and eightfold lower respectively in pregnant compared with cyclic ewes on day 16 post-oestrus.…”

Section: Resultsmentioning

confidence: 99%

“…Cyclic variations in endometrial steroid receptor concentrations have been described in cattle (Senior, 1975;Zelinski et al 1982;Meyer et al 1988), sheep (Koligian & Stormshak, 1977;Miller et al 1911b;Findlay et al 1982;Cherny et al 1991) and pigs (Deaver & Guthrie, 1980). Particularly well-charac¬ terized is the phenomenon of oestrogen-induced synthesis of both oestrogen and progesterone recep¬ tors (Aronica & Katzenellenbogen, 1991), with con¬ centrations of both receptors being greatest around oestrus in ewes (Koligian & Stormshak, 1977;Miller et al 1911b;Cherny et al 1991).…”

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confidence: 99%

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Changes in progesterone and oestrogen receptor mRNA and protein during maternal recognition of pregnancy and luteolysis in ewes

Ott

Zhou

Mirando

et al. 1993

Journal of Molecular Endocrinology

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This study characterized changes in levels of mRNA and protein for endometrial oestrogen receptors (ERs) and progesterone receptors (PRs) during luteolysis and maternal recognition of pregnancy. For cyclic and pregnant ewes, endometrium was collected on days 10, 12, 14, or 16 post-oestrus (4 ewes/day for each status) for the measurement of ER and PR mRNA and protein. The amount of receptor mRNA is expressed in relative units above background, measured from radiographs of dot-blot hybridization of total endometrial RNA with ER and PR cDNAs. At hysterectomy, jugular vein blood samples were collected and assayed for progesterone, total corpus luteum weight was recorded and, in vitro, endometrial oxytocin-stimulated inositol phosphate formation was estimated. In pregnant ewes, plasma progesterone increased gradually between days 10 and 16 (P < 0.01), corpus luteum weight was stable at approximately 0.8 g and oxytocin did not stimulate endometrial formation of inositol phosphates in vitro. In contrast, in cyclic ewes, plasma progesterone decreased from day 10 to day 16 (P < 0.01), corpus luteum weight decreased after day 14 to approximately 0.48 g (P = 0.05) and oxytocin stimulated an increase of approximately 1300% in the endometrial formation of inositol phosphates on day 16. cDNAs specifically hybridized with 1.6 and 3.1 kb transcripts for PR mRNA and a 6.5 kb transcript for ER mRNA. In cyclic ewes, the amount of PR mRNA increased from day 10 to maximum levels on days 14-16. The number of PRs decreased from day 10 (2.25 pmol/mg DNA) to day 12 (0.98 pmol/mg DNA) and then increased from day 14 to day 16 (2.8 pmol/mg DNA). In pregnant ewes, PR mRNA levels were greatest on days 10-12 and decreased by approximately 50% by day 16. In contrast, the number of PRs was relatively unchanged from day 10 to day 16 (1.53 to 1.03 pmol/mg DNA). In cyclic ewes, the amount of ER mRNA was lowest at day 10 and increased fivefold by day 16. The number of ERs remained relatively unchanged from day 10 to day 14 (6.07 pmol/mg DNA) and increased by day 16 (16.12 pmol/mg DNA). In pregnant ewes, ER mRNA decreased by approximately 80% from day 12 to day 16. Similarly, the number of ERs decreased from day 10 to day 16 (5.41 to 2.05 pmol/mg DNA). Correlations between ER mRNA and PR mRNA (r = 0.68), ERs and PRs (r = 0.50) and ER mRNA and ERs (r = 0.50) were high (P < 0.01).(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Changes in progesterone and oestrogen receptor mRNA and protein during maternal recognition of pregnancy and luteolysis in ewes

Ott

Zhou

Mirando

et al. 1993

Journal of Molecular Endocrinology

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“…None of these events, however, was observed when plasma progesterone concentrations were high in any of the heifers studied. The inhibition by progesterone of oestradiol-17ß-induced changes in the cervix and in oestrous behaviour may have been due to progester¬ one reducing the concentration of oestradiol-17ß receptors in tissue of the reproductive tract (Senior, 1975;Henricks & Harris, 1978) and brain (Feder & Marrone, 1977) respectively. The blockade of ovula¬ tion by progesterone was presumably caused by sup¬ pression of the obligatory oestradiol-17ß-induced preovulatory LH surge (Kesner, Padmanabhan & Convey, 1982;Schoenemann, Humphrey, Crowder et al 1985).…”

Section: Discussionmentioning

confidence: 99%

Effect of pulsatile infusion of gonadotrophin-releasing hormone on plasma oestradiol-17β concentrations and follicular development during naturally and artificially maintained high levels of plasma progesterone in heifers

Glencross¹

1987

Journal of Endocrinology

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To stimulate a follicular-phase pattern of pulsatile LH release, gonadotrophin-releasing hormone (GnRH; 5 micrograms) was infused (i.v.) hourly into heifers for periods of 5-11 days during the luteal phase of the oestrous cycle, and also when plasma progesterone levels were increased artificially by means of a progesterone-releasing intravaginal device. Plasma oestradiol-17 beta concentrations increased from basal (less than or equal to 2.5 pmol/l) to preovulatory peak levels (20-30 pmol/l) during the first 3 days of GnRH treatment. They were maintained at these values before returning to basal levels within 24 h of cessation of infusion. This response occurred regardless of the source of progesterone (endogenous or administered). Follicular development was observed by ovarian palpation (per rectum) in some heifers at the time of maximum secretion of oestradiol-17 beta. There was no detectable cervical mucus secretion or oestrous behaviour during these periods of high oestradiol-17 beta levels and ovulation did not occur. Treatment with GnRH did not affect plasma progesterone concentrations or oestrous cycle length. The study shows that oestradiol-17 beta secretion and follicular development (and the accompanying oestrus and ovulation) are suppressed during the luteal phase of the cycle by high concentrations of plasma progesterone, and provides strong indirect evidence that such inhibition is associated with a reduction in the pulse frequency of LH release.

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“…In studies performed around the end of 1970s and during the 1980s involving steroid binding assays and recent studies using immunohistochemistry and immunocytochemistry, it was reported that the ER and PR concentrations in bovine endometrium vary considerably during the oestrous cycle and they were found to be highest at oestrus or a few days later and to drop to lower values along luteal phase (Senior 1975; Henricks and Harris 1978; Zelinski et al. 1982; Meyer et al.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Detection of Receptors for Oestrogen and Progesterone in Endometrial Glands and Stroma during the Oestrous Cycle in Nelore (Bos taurus indicus) Cows

Martin

Neto

Oba

et al. 2008

Reprod Domestic Animals

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The aim of the present study was to monitor endometrial distribution and concentrations of oestrogen receptors alpha (ER alpha) and progesterone receptors (PR) by immunohistochemistry in Nelore cows (Bos taurus indicus) during the oestrous cycle. Blood samples were collected for progesterone measurement and endometrial samples were taken from the uterine horn contra lateral to the corpus luteum in 16 cows at days 0 (ovulation), 5, 9, 13 and 19 of the oestrous cycle. Immunostaining evaluation for ER alpha and PR in the glandular epithelium and uterine stroma was performed by two methods: positive nuclei counting and staining intensity of the nuclei. Specific positive staining reactions for both receptors were limited to cell nuclei and they were not identified in the cytoplasm. The proportion of ER alpha positive nuclei had a temporal variation throughout the oestrous cycle in both cell types evaluated and was higher in uterine stroma than the glandular epithelium (p < 0.05). The greatest proportion of ER alpha stained nuclei was observed at oestrus and during the initial and mid luteal phase (days 5, 9 and 13) (p < 0.05) in the glandular epithelium and at days 0, 5 and 9 in the uterine stroma (p < 0.01). The proportion of PR positive nuclei remained constant throughout the entire oestrous cycle for both cell types evaluated (p > 0.05). A higher proportion of PR positive nuclei was measured in the uterine stroma compared with the glandular epithelium (p < 0.05). Intensity of staining for ER alpha and PR varied throughout the oestrous cycle (p < 0.01). There was a higher staining intensity at days 0 and 5 in the stroma for ER alpha (p < 0.01) and PR (p < 0.01) and in the glandular epithelium at days 0, 5, 9 and 13 for ER alpha (p < 0.01) and at days 0, 5 and 9 for PR (p < 0.01) when compared with the other evaluated days. These data demonstrate that ER alpha and PR expression varied throughout the oestrous cycle in Nelore cows, in general with highest concentrations at oestrus and the lowest during the luteal phase. This is similar to patterns observed in Bos taurus taurus.

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Cytoplasmic Oestradiol-Binding Sites and Their Relationship to Oestradiol Content in the Endometrium of Cattle

Cited by 19 publications

References 26 publications

Changes in progesterone and oestrogen receptor mRNA and protein during maternal recognition of pregnancy and luteolysis in ewes

Changes in progesterone and oestrogen receptor mRNA and protein during maternal recognition of pregnancy and luteolysis in ewes

Effect of pulsatile infusion of gonadotrophin-releasing hormone on plasma oestradiol-17β concentrations and follicular development during naturally and artificially maintained high levels of plasma progesterone in heifers

Immunohistochemical Detection of Receptors for Oestrogen and Progesterone in Endometrial Glands and Stroma during the Oestrous Cycle in Nelore (Bos taurus indicus) Cows

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