Changes in progesterone and oestrogen receptor mRNA and protein during maternal recognition of pregnancy and luteolysis in ewes

Ott, Troy L.; Zhou, Yi; Mirando, Mark A.; Stevens, C.J.M.; Harney, Jacob P.; Ogle, Thomas F.; Bazer, Fuller W.

doi:10.1677/jme.0.0100171

Cited by 59 publications

(46 citation statements)

References 51 publications

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“…Estradiol up-regulates the ER mRNA levels in endometrium and myometrium of ovariectomized ewes [12,38]. The increases in ER mRNA and PR mRNA levels in this study were similar to those reported in intact cyclic ewes [27,35]. In the present study, the changes in mRNA levels of ER and PR were consistent with the receptor concentrations.…”

Section: Discussionsupporting

confidence: 89%

“…An example of this action is the regulation of the expression of their own receptor; the E-receptor complex stimulates both ER and PR gene expression in uteri of several mammalian species, while the P-receptor complex reduces levels of both receptors [4]. This is displayed by the variations in the concentrations of uterine sex steroid receptors [25,31] as well as their respective mRNAs during the estrous cycle of the sheep [27,35].…”

Section: Introductionmentioning

confidence: 99%

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A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

et al. 2000

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-Regulation of the uterine expression of estrogen and progesterone receptors was studied in 20 three-month-old lambs that were not treated or treated with estradiol-17β. Determinations of receptors were performed by binding assays in the nuclear and cytosolic fractions, receptor mRNAs by solution hybridization, and estrogen receptor protein by an enzyme-immunoassay. Estradiol treatment decreased the receptor binding capacity of both receptors and the levels of immunoreactive estrogen receptor 12 h after injection in the absence of decreased receptor mRNAs, suggesting that the initial decrease is due to degradation of the proteins or that mRNAs are translated into new receptor proteins at a reduced rate. The mRNA levels increased after estradiol treatment suggesting that the replenishment phase consists of synthesis of new receptors rather than recycling of inactivated receptors.estrogen / progesterone / receptor / uterus / lamb Résumé -Action biphasique de l'oestradiol sur l'expression des récepteurs aux oestrogènes et à la progestérone dans l'utérus de l'agnelle. La régulation de l'expression des récepteurs aux oestrogènes et à la progestérone au niveau de l'utérus a été étudiée chez des agnelles immatures âgées de 3 mois (n = 20), traitées et non traitées avec oestradiol-17β. Les déterminations des récepteurs ont été faites par des essais de liaison dans les fractions nucléaires et cytosoliques, les ARNm des récepteurs par la méthode d'hybridation en solution et la protéine du récepteur aux oestrogènes par un essai immuno-enzymatique. Le traitement à l'oestradiol réduit la capacité de liaison des deux récepteurs et les niveaux des récepteurs aux oestrogènes immunoréactifs 12 h après l'injection en absence d'une diminution des ARNm des récepteurs, suggèrent que la réduction initiale est due à la dégradation des Reprod. Nutr. Dev. 40 (2000) 283-293 283

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

et al. 2000

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“…Our initial hypothesis was that IFNT inhibited expression of ESR1 and OXTR and stabilized PGR in uterine epithelia to extend the period of the P4 block proposed by McCracken [89]. We found that steady-state levels of PGR mRNA were not different between pregnant and cyclic ewes [110]; however, in situ hybridization analysis revealed loss of expression of PGR mRNA and protein in all uterine epithelia by Day 13 of both the estrous cycle and pregnancy but continued expression of PGR mRNA and protein during pregnancy in both uterine stromal and myometrial cells [111]. Given that P4 is the hormone of pregnancy and that PGR are expressed only in stromal and myometrial cells, the concept that progestamedins from stromal cells affect uterine epithelia became important to our studies.…”

Section: Pregnancy and Pregnancy Recognition Signalingmentioning

confidence: 63%

“…Interferon tau (IFNT) is the pregnancy recognition hormone in sheep and other ruminants that acts to silence expression of estrogen receptor alpha (ESR1) and, in turn, oxytocin receptor (OXTR) to prevent development of the luteolytic mechanism that required oxytocin (OXT) from the corpus luteum (CL) and posterior pituitary to induce luteolytic pulses of prostaglandin F 2a (PGF). Thus, IFNT blocks the ability of the uterus to develop the luteolytic mechanism but does not inhibit prostaglandin synthase 2 (PTGS2) or the basal production of PGF during pregnancy [91,[94][95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110][111].…”

Section: Pregnancy and Pregnancy Recognition Signalingmentioning

confidence: 99%

Contributions of an Animal Scientist to Reproductive Biology

Bazer

2011

Biology of Reproduction

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I became interested in biology as an undergraduate in a premedical curriculum but developed a passion for the field of reproductive biology because of a course in physiology of reproduction taken to meet requirements for admission to veterinary school. My career path changed, and I entered graduate school, obtained the Ph.D., and have enjoyed an academic career as a reproductive biologist conducting research in uterine biology and pregnancy in animal science departments at the University of Florida and at Texas A&M University. However, I have never allowed academic boundaries to interfere with research and graduate education as that is contrary to collegiality, the cornerstone of great universities. I consider that my major contributions to science include 1) identification of proteins secreted by cells of the uterine endometrium that are critical to successful establishment and maintenance of pregnancy; 2) discovery of steroids and proteins required for pregnancy recognition signaling and their mechanisms of action in pigs and ruminant species; 3) investigation of fetal-placental development and placental transport of nutrients, including water and electrolytes; 4) identification of linkages between nutrition and fetal-placental development; 5) defining aspects of the endocrinology of pregnancy; and 6) contributing to efforts to exploit the therapeutic value of interferon tau, particularly for treatment of autoimmune diseases. My current studies are focused on the role of select nutrients in the uterine lumen, specifically amino acids and glucose, that affect development and survival of the conceptus and translation of mRNAs and, with colleagues at Seoul National University, gene expression by the avian reproductive tract at key periods postovulation. Another goal is to understand stromal-epithelial cell signaling, whereby progesterone and estrogen act via uterine stromal cells that express receptors for sex steroids to stimulate secretion of growth factors (e.g., fibroblast growth factors and hepatocyte growth factor) that, in turn, regulate functions of uterine epithelial cells and conceptus trophectoderm.

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“…Estrogen affects the oxytocin-induced PGF 2α production presumably through the up-regulation of the OTR receptor [46,47]. Estrogen receptor (ER) is up-regulated before luteolysis, which coincides with an increase in OTR [39,48,49]. Progesterone, essential for the maintenance of pregnancy, may also be required for the luteolytic pr oc ess.…”

Section: Biological Properties Of Ifnτ τ τ τmentioning

confidence: 99%

Regulation of Interferon-.TAU. Gene Expression and the Maternal Recognition of Pregnancy.

Yamaguchi

Nagaoka

Matsuda

et al. 2001

Journal of Reproduction and Development

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Abstract. In order for pregnancy to be established, the maternal system must recognize the presence of a conceptus(es) within the uterus. This physiological phenomenon, which subsequently causes the extension of the corpus luteum (CL) life-span, is known as maternal recognition of pregnancy. In ruminant ungulates, interferon-tau (IFNτ), produced by the conceptus, binds to the receptor located in the maternal endometrium in a paracrine manner. Its binding then affects levels of endometrial oxytocin receptor and estrogen receptor, which reduces pulsatile secretion of a luteolysin, prostaglandin F2α, resulting in the extension of CL function. Production of IFNτ seems to parallel the degree of trophoblast elongation and as the conceptus begins its attachment to the endometrium, IFNτ levels decline rapidly. Involvement of IFNτ in the process of implantation has long been suspected, but a role for IFNτ other than CL extension or transcriptional regulation of IFNτ genes has not been elucidated. In fact, several transcription factors that regulate the expression of IFNτ have been found, but none could explain its unique regulation. Recent findings related to maternal recognition of pregnancy are presented herein and a new insight into the potential regulation of IFNτ is discussed.

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Changes in progesterone and oestrogen receptor mRNA and protein during maternal recognition of pregnancy and luteolysis in ewes

Cited by 59 publications

References 51 publications

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

Contributions of an Animal Scientist to Reproductive Biology

Regulation of Interferon-.TAU. Gene Expression and the Maternal Recognition of Pregnancy.

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