Cytomorphology of Brassica napus × B. rapa hybrids and patterns of variation in the F2 derivatives

Choudhary, B. R.; Joshi, P.

doi:10.1080/00087114.2012.760878

Cited by 2 publications

(3 citation statements)

References 17 publications

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“…Orientation of the chromosomes at metaphase I and disjunction of chromosome at anaphase I and II was found abnormal (Meng et al, 1998;Souza, 1999;Gupta et al, 2014). In addition to these bridge-fragment con guration was also observed at anaphase I and anaphase II (Choudhary et al, 2012). As per the observations B. carinata performed better as a seed parent with B. nigra, B. juncea and B. rapa whereas, with B. napus it performed good as a pollen parent.…”

Section: Discussionsupporting

confidence: 73%

Pre-fertilization Barrier, Crossability and Meiotic Behavior of Interspecific Hybrids Among Brassica Species

Pant

Joshi

Lohani

et al. 2021

Preprint

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Inter-specific hybridization is an important driving force in plant evolution, speciation and creation of novel genetic variations those are not available with the naturally occurring species. Present investigation includes study of pre-fertilization barriers, phenotypic characterization and the meiotic abnormalities during microsporogenesis in the interspecific hybrids. Mean pollen tube germination percent in interspecific hybrids was recorded to be highest in case of B. carinata x B. nigra (33.67±11.35) and lowest between B. carinata x B. napus (18.68±6.62) whereas in the reciprocal crosses it was highest for B. napus x B. carinata (33.06±13.41). Siliqua set ranged from 33% in B. carinata x B. napus to 72% in selfedB. carinata. Seed set % ranged from 0% in B. rapa x B. carinata and B.nigra x B.carinatato 25.64 % for B. carinata x B.nigra. Mean pollen tube growth was recorded highest in B. carinata x B. nigra (21.23±5.33) while was lowest in B. carinata x B. napus (10.18±3.17) and among reciprocals it was highest for B. nigra x B. carinata (21.39±7.53). Pollen tube abnormalities viz., coiling and bending of pollen tube, hairpin shaped pollen tube, swelling of tube tip, tube bifurcation, more than one tube emerging from pollen, tubes growing in wrong direction were observed. B. carinata as pollen parent showed highest mean pollen tube abnormality percent with B. napus (36.32±8.62) whereas lowest with B. nigra (18.09±6.73). It was observed highest with B. rapa (49.170 ±13.55) when B. carinata was used as pollen parent. Some of the pre fertilization barriers related to stylar and ovule region can also be observed through fluroscent microscopy. As per the observations B. carinata performed better as a seed parent with B. nigra, B. juncea and B. rapa whereas, with B. napus it performed good as a pollen parent. Correlation study gave the association the above traits with each other. The meiotic abnormalities showed improper alignment at M-I, M-II, A-I, A-II and Telophase.

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Section: Discussionsupporting

confidence: 73%

Pre-fertilization Barrier, Crossability and Meiotic Behavior of Interspecific Hybrids Among Brassica Species

Pant

Joshi

Lohani

et al. 2021

Preprint

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“…Reciprocal crosses were generally unsuccessful. Moreover, Choudhary et al (2012) demonstrated that amongst crosses involving three varieties/forms of B. rapa with B. napus, yellow sarson produced a maximum number of hybrids (18.0%) followed by brown sarson (11.3%) and toria (6.9%). Thus, the success rate of cross-fertility of three varieties of B. rapa with B. napus was in the order yellow sarson > brown sarson > toria.…”

Section: Discussionmentioning

confidence: 99%

“…To date, successful attempts of hybridization between Brassica rapa and Brassica napus have been published in numerous scientific papers (Fitz et al, 2007;Choudhary et al, 2012). Crossability between B. napus and B. rapa has also been examined, specifically in terms of the genes introgression from B. napus to B. rapa , which is mainly considered a weed in America and Europe (Tsuda et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Interspecific hybridization between Brassica napus and Brassica rapa ssp. chinensis genotypes through embryo rescue and their evaluation for crossability

Niemann

Olender

Wojciechowski

et al. 2015

bta

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The genus Brassica contains several important crop species that are used for a variety of purposes. Brassica napus and Brassica rapa are the most important ones for their use as oilseed crops. In Brassica , interspecific hybridization is a potential and useful method for transferring valuable traits between species of commercial interest. For breeding Brassicas resistant to some diseases, i.e. clubroot, attempts of transferring resistant genes (CR) through interspecific hybridization have also been reported. The main step in the introgression process is the production of interspecific hybrids between the two species. However, the cross-incompatibility occurring in wide hybridization might hamper the possibility of obtaining hybrid progenies. For that reason, the crossability study is essential and may give an insight into the cross-compatibility relationship among the species, the direction of success of crossing, and the crossability barriers of some combinations, if any. In the present study, interspecific reciprocal crosses between B. napus and B. rapa ssp. chinensis genotypes were carried out in order to determine their crossability and to produce their F1 hybrids. Crossability was analyzed based on the pollen germination index (PGI) and the development of hybrid embryos in in vitro cultures. It was observed that the unilateral interspecific incompatibility occurred in crosses between B. napus × B. rapa ssp. chinensis. When B. napus cultivars were used as a maternal parent, pollen grains of B. rapa germinated well on the stigmas, while only a small number of pollen tubes could elongate near the ovules, resulting in a low development of hybrid embryos. Using the B. rapa ssp. chinensis as the pistillate parent in the crosses, it was found that the crossability was relatively higher than that in the case of the reciprocal cross. This resulted in a greater number of embryos obtained. Generally, it was concluded that the selection of parental components for hybridization is an important step for obtaining breeding success.

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Cytomorphology of Brassica napus × B. rapa hybrids and patterns of variation in the F₂ derivatives

Cited by 2 publications

References 17 publications

Pre-fertilization Barrier, Crossability and Meiotic Behavior of Interspecific Hybrids Among Brassica Species

Pre-fertilization Barrier, Crossability and Meiotic Behavior of Interspecific Hybrids Among Brassica Species

Interspecific hybridization between Brassica napus and Brassica rapa ssp. chinensis genotypes through embryo rescue and their evaluation for crossability

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