Cytomorphological Analysis of Keratinocytes in Oral Smears from Tobacco Users and Oral Squamous Cell Carcinoma Lesions — A Histochemical Approach

Khandelwal, Suneet; Solomon, Monica Charlotte

doi:10.4248/ijos10011

Cited by 33 publications

(36 citation statements)

References 15 publications

(11 reference statements)

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“…Keeping in mind the other conditions like candidiasis, smoking, alcohol, oral premalignant and malignant conditions linked with cytomorphometric changes as well. [10][11][12][13][14] Additional work is desirable to determine the association among these disorders. Knowledge of both quantitative and qualitative alterations in oral epithelial cells of diabetic patients is also important as these alterations are similar to that seen in precancerous and radiation-induced changes.…”

Section: Discussionmentioning

confidence: 99%

Detection of Cytomorphological Changes in Buccal Mucosal Cells of Type 2 Diabetic Patients Using Oral Exfoliative Cytology

Sharif

Sarwar²,

Nisar³

et al. 2017

Annals KEMU

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Background: Diabetes mellitus is an extremely common endocrine metabolic disorder that results in chronic hyperglycemia. It has effects on various tissues of the body. Due to this increased blood glucose levels considerable cellular changes occur in oral cavity as well. This field has attracted little research. The aim of the study was to analyze the changes in morphology and cytomorphometric measurements in the buccal mucosal cells of type 2 diabetic patients.

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Section: Discussionmentioning

confidence: 99%

Detection of Cytomorphological Changes in Buccal Mucosal Cells of Type 2 Diabetic Patients Using Oral Exfoliative Cytology

Sharif

Sarwar²,

Nisar³

et al. 2017

Annals KEMU

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“…Quantitative assessment of cytological nuclear features can play an important role in preoperative assessment and complement morphological features in thyroid lesions (Priya and Sundaram, 2011). Cytomorphometry in oral smears is proving to be a useful adjunct in the early diagnosis of oral cancer (Khandelwal et al, 2010).…”

Section: 6919 Utility Of Nuclear Morphometry With Imagej In Effusionmentioning

confidence: 99%

Utility of Nuclear Morphometry in Effusion Cytology

Ambroise

Jothilingam²,

Ramdas³

2014

Asian Pacific Journal of Cancer Prevention

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Background: The cytological analysis of serous effusions is a common investigation and yields important diagnostic information. However, the distinction of reactive mesothelial cells from malignant cells can sometimes be difficult for the cytopathologist. Hence cost-effective ancillary methods are essential to enhance the accuracy of cytological diagnosis. The aim of this study was to examine the utility of nuclear morphometry in differentiating reactive mesothelial cells from malignant cells in effusion smears. Materials and Methods: Sixty effusion smears consisting of 30 effusions cytologically classified as malignant (adenocarcinomas) and 30 benign effusions showing reactive mesothelial cells were included in the study. ImageJ was used to measure the nuclear area, perimeter, maximal feret diameter, minimal feret diameter and the circularity. A total of ten representative cells were studied in each case. Results: Significant differences were found between benign and malignant effusions for the nuclear area, perimeter, maximal feret diameter and minimal feret diameter. No significant difference was found for circularity, a shape descriptor. Receiver operating characteristic (ROC) curve analysis revealed that nuclear area, perimeter, maximal feret diameter, and minimal feret diameter are helpful in discriminating benign and malignant effusions. Conclusions: Computerised nuclear morphometry is a helpful ancillary technique to distinguish benign and malignant effusions. ImageJ is an excellent cost effective tool with potential diagnostic utility in effusion cytology.

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“…13 Thus, this reduction in CD could be an early indication of malignant change and these lesions can be considered as precancerous lesions. 10,11,12 Ramaesh et al reported a significant increase in the nuclear size in normal mucosal lesions with no dysplasia and further through dysplasias to squamous cell carcinoma. 11,12 Similar findings of an increase in nuclear size with dysplasia and squamous cell carcinoma have been reported in cervical smears and in experimental lesions in hamster cheek.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the amount of cytoplasm the cell makes decreases relative to the amount of nucleoplasm, so that the cell diameter decreases and there is an actual increase in nuclear size which may be related to an increase in the nuclear contents required for replication. 10 As a result, the nuclear-to-cytoplasmic ratio increases at times to an extreme degree. Such examples of increased activity are seen as a result of various processes acting on the cells including stimulation, replication and repair from degeneration, premalignant processes and neoplasia.…”

Section: Discussionmentioning

confidence: 99%

“…11,12 Similar findings of an increase in nuclear size with dysplasia and squamous cell carcinoma have been reported in cervical smears and in experimental lesions in hamster cheek. 13 Thus, this increase in ND could be an early indication of malignant change 10,11 and case groups II and III were intermediate stages in the range from normal to carcinoma.…”

Section: Discussionmentioning

confidence: 99%

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Cytomorphometric Analysis of Exfoliative Palatal Mucosal Cells in Reverse Smokers in 4 Villages of Srikakulam District, Andhra Pradesh - An Observational Exfoliative Study

Lavanya¹,

Suresh²

2016

jemds

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Squamous cell carcinoma of palate is found to be the rarest of all the cancers. But the prevalence of palatal cancers in Srikakulam and Visakhapatnam districts (Coastal districts of Andhra Pradesh) was found to be 0.1% 1,2 and noticed to be associated with the habit of reverse smoking in these geographic areas. These palatal cancers were found to arise from the pre-existing precancerous lesions namely Excrescences, White patches and Red areas, which were designated as Reverse smoker's lesions. AIM OF THE STUDYAn observational cytomorphometric analysis study has been undertaken in this region to study quantitatively nuclear diameter, cell diameter and nuclear-to-cytoplasmic ratio from exfoliative smears of various reverse smoker's lesions to assess the type of reaction of palatal mucosal cells to the heat generated during reverse smoking. MATERIALS AND METHODSMaterials used were mouth mirror, tongue depressor, wooden spatula, glass slides, 95% alcohol, rapid PAP kit, Coplin jars, DPX mountant, xylene, coverslips, microscope and micrometre. The smears were collected using wooden spatula from various palatal lesions and fixed in 95% alcohol. Staining of smears was done using rapid PAP kit. The cellular diameter and nuclear diameter of the cells were measured using micrometre and microscope. RESULTSThe mean cellular diameter of White areas and Red areas was decreased compared to normal mucosa cells and the nuclear diameter was increased compared to normal mucosa cells. Both the diameters of both the lesions were nearing to carcinoma cells. The cellular and nuclear diameter of the excrescences were increased when compared to normal mucosa and carcinoma cells. CONCLUSIONExcrescences represent proplastic and hyperplastic reaction of palatal surface epithelium, whereas white areas and red areas represent premalignant changes of palatal surface epithelium. (Figure 1). The rarest oral site is found to be palate, but still high prevalence of palatal cancer in Srikakulum and Visakhapatnam districts was noticed by many research workers. This was found to be associated with the habit of reverse smoking practiced in this geographic area. 2,3,4,5 (Figure 2). The palatal changes produced by reverse smoking are multimorphic consisting of several components namely Financial or Other, Competing Interest: None. Submission 02-08-2016, Peer Review 19-08-2016, Acceptance 22-08-2016, Published 26-08-2016. Corresponding Author: Dr. Sirigala Lavanya, Professor in Charge, Department of Oral Pathology, Government Dental College and Hospital, Kadapa. E-mail: slavanyamds@yahoo.co.in DOI: 10.14260/jemds/2016/1124 Palatal keratosis, Excrescences, Red patches, Ulceration and finally Carcinoma. 1,3,4,5 The malignant potential of these lesions varies and cellular changes in these lesions can be observed in the exfoliated cells. These palatal changes were due to both tobacco by-products and heat generated above 120 0 C during reverse chutta smoking. KEYWORDSThe optimal outcome in the management of any malignancy is dependent upon...

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Cytomorphological Analysis of Keratinocytes in Oral Smears from Tobacco Users and Oral Squamous Cell Carcinoma Lesions — A Histochemical Approach

Cited by 33 publications

References 15 publications

Detection of Cytomorphological Changes in Buccal Mucosal Cells of Type 2 Diabetic Patients Using Oral Exfoliative Cytology

Detection of Cytomorphological Changes in Buccal Mucosal Cells of Type 2 Diabetic Patients Using Oral Exfoliative Cytology

Utility of Nuclear Morphometry in Effusion Cytology

Cytomorphometric Analysis of Exfoliative Palatal Mucosal Cells in Reverse Smokers in 4 Villages of Srikakulam District, Andhra Pradesh - An Observational Exfoliative Study

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