Peritoneal macrophages of mice, 48 hr after intraperitoneal administration of albumin (egg white), were studied.Almost all granules in the cytoplasm of macrophages stained by the supravital staining with neutral red were revealed to be colored brick red by the staining with Sudan III in the smear preparation.Because of lack of lipids in the inoculum, it is difficult to explain that these granules stained with Sudan III were lipids phagocytosed.The observation led the suggestion that the Sudan III stained granules in macrophages were out of all relation to lipids phagocytosed and that the granules indicated some functional phase of macrophages.-macrophages; Sudan III stained granules; atherosclerosisIn general, the granules of macrophages stained with Sudan III have been considered to be lipids phagocytosed. In a series of the cytological study of lepromatous leprosy, Usubuchi and Arakawa (1956) and Arakawa (1958) reported that the Sudan III stained granules in lepra cells were also stained by the supravital staining with neutral red. From this finding they raised a question about the prevalent view that the granules stained with Sudan III in lepra cells were derived, by phagocytosis, from lipids of Mycobacterium leprae (Harada 1955).The question of the same kind about Sudan III stained granules of macrophages was brought out in various diseases besides leprosy.We report the evidence that almost all granules of macrophages appearing in the peritoneal cavity in a short time after administration of albumin were stained with Sudan III.
MATERIALS AND METHODSTwenty male dd mice weighing 20 to 30 g were used. Before the treatment, smear preparations of cells in the ascites of all mice were microscopically observed by Giemsa staining. Animals were divided into two groups each consisting of 10 mice. In the first group, 0.2 ml of 1% albumin (eggwhite, Sigma Chem. Co.) dissolved in the physiological saline solution was administered into the peritoneal cavity. In the second, 0.2 ml of 10% India ink (Kuretake Sumi Honpo Co., Tokyo) dissolved in the physiological saline solution was injected into the peritoneal cavity.Forty-eight hr after administration, the observation of ascites cells was done by Giemsa staining of the smear preparation and by the supravital staining with neutral red. The Sudan III-hematoxylin staining of the smear preparation was also carried out.