Cytologic and immunocytochemical characterization of feline progressive histiocytosis

Abstract

FPH is a distinctive neoplastic lesion composed of nonphagocytizing histiocytes variably admixed with neutrophils and small mature lymphocytes. Immunocytochemical analysis with CD1 is mandatory to confirm a dendritic cell origin. Immunocytochemistry and cytomorphology allowed the specific and rapid diagnosis of FPH on cytologic samples.

Clinical outcome,PDGFRβandKITexpression in feline histiocytic disorders: a multicentre study

“…This cat is disease‐free (follow‐up, 465 days). It may be that in this case immune‐mediated mechanisms are involved, as in reactive histiocytosis in dogs, or a Langherans tumour, behaving like canine histiocytoma: testing for E‐cadherin was negative, however, variable expression of this marker has been found in two different studies on FPH …”

“…A recent study on FPH using immunocytochemistry (ICC) reported that neoplastic cells expressed CD1a, CD1c, CD11b, CD18 and MHCII . CD1 was needed to confirm a dendritic cell origin, but this marker is of limited use on formalin fixed samples . In contrast to previous studies, E‐cadherin expression was reported in 4 out of 5 cats with FPH at presentation.…”

“…IHC using panels of leukocyte and histiocytic markers can differentiate histiocytic disease from the more common feline round cell tumours [lymphoma, mast cell tumour (MCT)]. A recent study on FPH using immunocytochemistry (ICC) reported that neoplastic cells expressed CD1a, CD1c, CD11b, CD18 and MHCII . CD1 was needed to confirm a dendritic cell origin, but this marker is of limited use on formalin fixed samples .…”

“…Feline progressive histiocytosis (FPH) is the form most commonly described in the literature . Affected cats present with solitary or multiple nodules that often disseminate to internal organs including liver, spleen and bone marrow.…”

Bimodal immunoglobulin A gammopathy in a cat with feline myeloma-related disorders

“…Those antibodies were proven to be specific to the feline antigen. A previous report showed the specificity of anti-feline CD18 monoclonal antibody and anti-human CD3 polyclonal antibody [ 22 ]. The specificities of the remaining antibodies were tested by IHC with normal feline lymph node (data not shown).…”