Dermal and Subcutaneous Masses

Brazzell, Jennifer L.; Heinrich, Daniel A.; Walz, J.

doi:10.1002/9781119380559.ch12

Cited by 3 publications

(2 citation statements)

References 137 publications

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“…Compared with the neoplastic cells, the low degree of atypical features, represented primarily by mild anisokaryosis, fits the benign nature of reactive fibroblastic cells. Although fibroblasts have been reported to show varying degrees of cellular atypia, ranging from mild to marked 16 with marked atypia characterized by many nuclear and cytoplasmic criteria of malignancy (anisocytosis, anisokaryosis, multinucleation, prominent and multiple nucleoli, and increased mitotic figures), we saw fibroblasts with mild, moderate, and severe atypia in 16/25, 8/25, and 1/25 cases, respectively.…”

Section: Discussionmentioning

confidence: 54%

Cytologic features of reactive fibroplasia in cutaneous and subcutaneous lesions of dogs: A retrospective study

Masserdotti¹,

Drigo²

2022

Veterinary Clinical Pathol

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BackgroundDetermining reactive fibroplasia from primary neoplastic proliferation can be difficult using the cytologic features of spindle cells. ObjectivesThis study aimed to describe qualitative features of benign reactive spindle cells and provide a diagnostic tool to distinguish between fibroplasia and soft tissue sarcoma.MethodsCytologic samples from cutaneous lesions with histopathologically confirmed fibroplasia were reviewed and compared with cytologic samples from confirmed low‐grade soft tissue sarcomas. The samples were obtained from the Archive of the Laboratorio Veterinario Bresciano (Brescia; Italy). ResultsA total of 25 cytologic samples retrieved from the database fit the selection criteria. For comparison, 25 cytologic samples of histopathologically confirmed soft tissue sarcomas with secondary inflammation were obtained and reviewed from the same database. A low number of singularly distributed spindle cells with mild features of atypia and a high ratio of inflammatory to spindle cells was observed in confirmed cases of fibroplasia. ConclusionsLow cellularity, individualized spindle cells, and an increased inflammatory‐to‐spindle cell ratio were more indicative of fibroplasia than sarcoma.

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Section: Discussionmentioning

confidence: 54%

Cytologic features of reactive fibroplasia in cutaneous and subcutaneous lesions of dogs: A retrospective study

Masserdotti¹,

Drigo²

2022

Veterinary Clinical Pathol

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“…Future research should consider larger sample sizes and standardization of immunohistochemistry procedures to elucidate their diagnostic utility further. As proposed, the incorporation of sophisticated molecular methods could provide a more comprehensive understanding of the parasite's molecular profile, adding specificity to diagnostic processes (20). Undertaking multicenter studies would address the inherent limitations of single-center studies by incorporating diverse clinical settings and bolstering the generalizability of the results.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Accuracy of Cd1a Immuno Histochemical Evaluation in Comparison with H & E and Giemsa for Cutaneous Leishmaniasis

Akbar,

Qadir,

Khan

2023

JHRR

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Background: Cutaneous leishmaniasis remains a global health challenge, with diagnosis largely dependent on the visualization of Leishmania parasites in infected tissue. Traditional staining methods, such as Hematoxylin and Eosin (H&E) and Giemsa, have been the cornerstone of histopathological diagnosis, but they come with limitations in sensitivity and specificity. Objective: This study aimed to evaluate the diagnostic accuracy of CD1a immunohistochemical staining in the detection of leishmania amastigotes in skin biopsies and compare its efficacy with H&E and Giemsa staining methods. Methods: A total of 110 skin biopsy samples with a strong clinical suspicion of cutaneous leishmaniasis were examined using H&E, Giemsa, and CD1a immunohistochemical staining techniques. The sensitivity and specificity of these methods were analyzed and compared to ascertain the most effective diagnostic approach. Results: The study identified leishmania amastigotes in 78% of the cases using H&E staining, while Giemsa staining resulted in amastigote detection in 82% of cases. Notably, CD1a immunohistochemical staining showed a remarkable sensitivity, identifying amastigotes in 95% of the samples. The increase in detection rate with CD1a staining was statistically significant when compared to H&E (p<0.05), with CD1a revealing amastigotes in an additional 23% of cases where H&E staining was negative. Giemsa staining was able to detect amastigotes in three cases that were not visible with H&E, suggesting its potential role as a complementary technique in certain diagnostic scenarios. Conclusion: The study concludes that CD1a immunohistochemical staining significantly enhances the detection of leishmania amastigotes and should be considered as an adjunct to conventional staining methods in the histopathological diagnosis of cutaneous leishmaniasis. Future diagnostic protocols could benefit from the incorporation of CD1a staining to improve accuracy.

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Cytological and complete blood count profile in a dog with suspected skin tumours

Mutiara,

Wijaya,

Prasaja

et al. 2024

ARSHI vet lett

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Skin tumours are among the most prevalent neoplasms in older dogs, and often display diverse clinical signs. This case report outlines the clinical presentation, diagnosis, and treatment of a suspected skin tumour in a 10-year-old obese female Golden Retriever weighing 46 kg. The owner brought the dog to West Java Provincial Animal Hospital with mandibular swelling, decreased appetite, and tachypnoea. Physical examination revealed a rectal temperature of 40.1°C, painful mandibular swelling, respiratory rate of 236 breaths per minute, and heart rate of 88 beats per minute. Cytology revealed pleomorphism, coarse chromatin, and nuclear moulding, suggesting increased cellular activity. Haematological results showed leukocytosis, granulocytosis, and hyperchromic normocytic anaemia, likely due to the release of proinflammatory cytokines from the tumour and immune cells. Differential diagnosis included sialadenitis, apocrine gland tumours, and salivary gland adenocarcinoma. The final diagnosis was skin tumour with poor prognosis. The treatment involved iron dextran, supplements, dexamethasone, sulfadiazine-trimethoprim, cyproheptadine HCl, and tolfenamic acid

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Dermal and Subcutaneous Masses

Cited by 3 publications

References 137 publications

Cytologic features of reactive fibroplasia in cutaneous and subcutaneous lesions of dogs: A retrospective study

Cytologic features of reactive fibroplasia in cutaneous and subcutaneous lesions of dogs: A retrospective study

Diagnostic Accuracy of Cd1a Immuno Histochemical Evaluation in Comparison with H & E and Giemsa for Cutaneous Leishmaniasis

Cytological and complete blood count profile in a dog with suspected skin tumours

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