Cytokinin as an Inducer of Vitrification in Melon

Leshem, B.; Shaley, D. P.; Izhar, S.

doi:10.1093/oxfordjournals.aob.a087552

Cited by 41 publications

(29 citation statements)

References 19 publications

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“…There are many reports indicating that during successive subcultures the number of abnormal plantlets increases, reaching in some cases 50-80% (Ziv et al 1983;Leshem et al 1988;Safrazbekyan et al 1990). Poor growth of abnormal shoots, low percentage of rooting and poor survival after transplantation into the soil limit commercial application of micropropagation methods for some crops.…”

Section: Introductionmentioning

confidence: 99%

Effect of applied and internal hormones on vitrification and apical necrosis of different plants cultured in vitro

Kataeva

Alexandrova

Butenko

et al. 1991

Plant Cell Tiss Organ Cult

100

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Development of vitrification and apical necrosis was followed in Camellia sinensis, Gerbera jamesonii, Malus domestica and hybrid Populus tremula × P. alba shoots cultured in vitro on Murashige & Skoog (MS) medium with different concentrations of growth regulators. High humidity in the culture vessels and excess of BA in the medium were found to be the major factors influencing vitrification. Lack of exogenous cytokinin in the medium during successive subcultures induced apical necrosis in poorrooting species (Malus domestica, Camellia sinensis). The level of internal phytohormones (ABA, IAA, IPA, 2iP, Z, ZR) was determined in the apple shoots by means of ELISA. The content of internal cytokinins in the vitrified apple shoots was several times greater than in normal ones, which supports the hypothesis that excess of cytokinins, inducing rapid divisions of cells in meristems in the atmosphere with high humidity, is responsible for vitrification. Apical necrosis of the plantlets that appeared after cultivation on cytokinin-free medium is the result of deficiency in endogenous hormones in apple shoots and this being confirmed by analysis of endogenous hormones in apple shoots.

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Section: Introductionmentioning

confidence: 99%

Effect of applied and internal hormones on vitrification and apical necrosis of different plants cultured in vitro

Kataeva

Alexandrova

Butenko

et al. 1991

Plant Cell Tiss Organ Cult

100

View full text Add to dashboard Cite

show abstract

“…Leshem et al (1988) suggest that vitrification is the bud's reaction to an excess of cytokinin. The beneficial effect of raising agar concentration should be explained by decreasing the availability of cytokinins.…”

Section: Culture Mediummentioning

confidence: 99%

“…It is accompanied by low rates of multiplication which finally cannot be pursued. On solid medium, all the vitrified plantlets turn into callus after two or three subcultures (Leshem et al 1988). Vitrified shoots do not root easily either in vitro or during their attempted weaning in soil.…”

Section: Disadvantagesmentioning

confidence: 99%

“…The vitrification symptoms in calli have been questioned in relation to habituation or auxin autonomy (Crevecoeur et al 1987;Gaspar et al 1988). The more simple question of whether callus formation in fact derives from a process of vitrification has also already been posed (Lamproye et al 1986); in many instances vitrified plantlets indeed finally turn into callus (Leshem et al 1988). Vitrification was found later to be accompanied by high potential multiplication rates under particular circumstances (John 1986).…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

See 1 more Smart Citation

Vitrification in Micropropagation

Gaspar

1991

Biotechnology in Agriculture and Forestry

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“…Os eventuais fatores da vitrificação têm sido atribuídos ao potencial osmótico (DEBERGH et al, 1981;HAKKAART & VERSLUIJS, 1983;PASQUALETO et al, 1988), concentração dos reguladores de crescimento, especialmente a citocinina (LESHEM et al, 1988a;1988b), umidade e a concentração salina do meio de cultura (DAGUIN & LETOUZÉ, 1986). Pára contribuir ao estudo dessa questão é que foi conduzido o presente trabalho tendo em vista determinar as condições controladoras da vitrifícação do cravo in vitro.…”

Section: Introductionunclassified