Cytokine loaded layer-by-layer ultrathin matrices to deliver single dermal papilla cells for spot-by-spot hair follicle regeneration

Lin, Bojie; Wang, Jin; Miao, Yong; Liu, Yuqing; Jiang, Wei; Fan, Zhexiang; Darabi, Mohammad-Ali; Hu, Zhiqi; Xing, Malcolm

doi:10.1039/c5tb02265g

Cited by 25 publications

(29 citation statements)

References 59 publications

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“…Isolation and Culture of DPCs : Vibrissae follicles of 4–6 week old C57BL/6J mice were used for isolation of DPs. DPCs were obtained and expanded as described previously, that isolated DPs were placed on the plastic dishes, and cultured in the DMEM supplemented with 1% (v/v) penicillin–streptomycin and 10% (v/v) FBS at 37 °C in a humidified 5% CO 2 atmosphere. The culture medium was exchanged every 2 d. Once DPCs outgrowth reached to 70–80% confluence, harvested with 0.25% (w/v) trypsin/EDTA, and then subculture was conducted with a split ratio of 1:2.…”

Section: Methodsmentioning

confidence: 99%

“…Establishment of DP Spheroids by LbL Nanoencapsulation on Single DPC : The procedure of LBL nanocoating on single DPC is as described in a previous study . Briefly, 1 × 10 6 DPCs were centrifuged to remove the medium within a centrifuge tube.…”

Section: Methodsmentioning

confidence: 99%

“…Layer‐by‐layer (LbL) self‐assembly has emerged as an approach in cell surface engineering . Multiple cells in each microgel with excessive gel disposition on cell surface may be detrimental to the fragile cells .…”

Section: Introductionmentioning

confidence: 99%

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Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

Wang

Miao

Huang

et al. 2017

Adv Healthcare Materials

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Cell surface engineering technology advances cell therapeutics and tissue engineering by accurate micro/nanoscale control in cell-biomaterial ensembles and cell spheroids formation. By tailoring cell surface, microgels can encapsulate cells for versatile uses. However, microgels are coated in a thick layer to house multiple cells together but not a single cell based. Besides, excessive deposition on cell surface is detrimental to cellular functions. Herein, layer-by-layer (LbL) self-assembly to encapsulate single cell using nanogel is reported, owing to its security and tunable thickness at nanoscale, and further forms cell spheroids by physical cross-linking on nanogel-coated cells for delivery. A hair follicle (HF) regeneration model where the dermal papilla cells (DPCs) are given a 3D installation to maintain its ability of HF induction during in vitro culture is studied. Dermal papilla (DP) spheroids are optimized and that LbL-DPCs aggregation is akin to primary DP is demonstrated. The markers ALP, Versican, and NCAM are examined to investigate that high-passaged (P8) DP spheroids can restore the hair induction potential, which are lost in 2D culture. New HFs are regenerated successfully by implantation of DP spheroids in vivo.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

Wang

Miao

Huang

et al. 2017

Adv Healthcare Materials

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“…GAG coating creates tissue microenvironment for DPCs that can sustain immunological and mechanical obstacles, supporting generation of hair follicle. Transplantation of GAG-coated DPCs leads to abundant hair growth and maturation of hair follicle, where GAG coating serves as ECM, enhancing intrinsic therapeutic potential of DPCs [ 58 ]. During infection, the inflammatory cytokines secreted from host immune cells attract MSCs to the site of inflammation, which modulates inflammatory responses, representing MSCs as key candidate of regenerative medicine for infectious disease therapeutics.…”

Section: Mscs/stromal Cells In Regenerative Medicinementioning

confidence: 99%

Stem Cells Applications in Regenerative Medicine and Disease Therapeutics

Mahla

2016

International Journal of Cell Biology

450

294

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Regenerative medicine, the most recent and emerging branch of medical science, deals with functional restoration of tissues or organs for the patient suffering from severe injuries or chronic disease. The spectacular progress in the field of stem cell research has laid the foundation for cell based therapies of disease which cannot be cured by conventional medicines. The indefinite self-renewal and potential to differentiate into other types of cells represent stem cells as frontiers of regenerative medicine. The transdifferentiating potential of stem cells varies with source and according to that regenerative applications also change. Advancements in gene editing and tissue engineering technology have endorsed the ex vivo remodelling of stem cells grown into 3D organoids and tissue structures for personalized applications. This review outlines the most recent advancement in transplantation and tissue engineering technologies of ESCs, TSPSCs, MSCs, UCSCs, BMSCs, and iPSCs in regenerative medicine. Additionally, this review also discusses stem cells regenerative application in wildlife conservation.

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“…Apart from probiotics delivery, LbL self‐assembly technique can also be utilized to encapsulate therapeutic cells with functional molecules incorporated multilayer shells to achieve simultaneous delivery of cells and regulatory factors . For example, our group previously reported the incorporation of insulin‐like growth factor‐1 (IGF‐1) into a gelatin/alginate multilayer shell which encapsulated the neural stem cells (NSCs), as shown in Figure D.…”

Section: Biomedical Applications Of Lbl Self‐assembly For Cell Encapsmentioning

confidence: 99%

Biomedical Applications of Layer‐by‐Layer Self‐Assembly for Cell Encapsulation: Current Status and Future Perspectives

Liu

Wang

Zhong

et al. 2018

Adv Healthcare Materials

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110

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Encapsulating living cells within multilayer functional shells is a crucial extension of cellular functions and a further development of cell surface engineering. In the last decade, cell encapsulation has been widely utilized in many cutting‐edge biomedical fields. Compared with other techniques for cell encapsulation, layer‐by‐layer (LbL) self‐assembly technology, due to the versatility and tunability to fabricate diverse multilayer shells with controllable compositions and structures, is considered as a promising approach for cell encapsulation. This review summarizes the state‐of‐the‐art and potential future biomedical applications of LbL cell encapsulation. First of all, a brief introduction to the LbL self‐assembly technique, including assembly mechanisms and technologies, is made. Next, different cell encapsulation strategies by LbL self‐assembly techniques are explained. Then, the biomedical applications of LbL cell encapsulation in cell‐based biosensors, cell transplantation, cell/molecule delivery, and tissue engineering, are highlighted. Finally, discussions on the current limitations and future perspectives of LbL cell encapsulation are also provided.

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Cytokine loaded layer-by-layer ultrathin matrices to deliver single dermal papilla cells for spot-by-spot hair follicle regeneration

Abstract: Polymer nanocoated dermal papilla cells promoting hair regeneration.

Cited by 25 publications

References 59 publications

Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

Stem Cells Applications in Regenerative Medicine and Disease Therapeutics

Biomedical Applications of Layer‐by‐Layer Self‐Assembly for Cell Encapsulation: Current Status and Future Perspectives

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