Stem Cells Applications in Regenerative Medicine and Disease Therapeutics

Mahla, Ranjeet Singh

doi:10.1155/2016/6940283

Cited by 450 publications

(321 citation statements)

References 125 publications

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“…Fortuitously, innovations in intestinal stem-cell culture methods have made it possible to use primary, non-transformed cells to create specialized tissues in vitro [34, 35]. With these methods, primary animal and human intestinal stem cells can be used to create multi-cellular structures known as enteroids that mimic many of the aspects of the small intestinal epithelium [11, 36].…”

Section: Introductionmentioning

confidence: 99%

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium

et al. 2017

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The human small intestinal epithelium possesses a distinct crypt-villus architecture and tissue polarity in which proliferative cells reside inside crypts while differentiated cells are localized to the villi. Indirect evidence has shown that the processes of differentiation and migration are driven in part by biochemical gradients of factors that specify the polarity of these cellular compartments; however, direct evidence for gradient-driven patterning of this in vivo architecture has been hampered by limitations of the in vitro systems available. Enteroid cultures are a powerful in vitro system; nevertheless, these spheroidal structures fail to replicate the architecture and lineage compartmentalization found in vivo, and are not easily subjected to gradients of growth factors. In the current work, we report the development of a micropatterned collagen scaffold with suitable extracellular matrix and stiffness to generate an in vitro self-renewing human small intestinal epithelium that replicates key features of the in vivo small intestine: a crypt-villus architecture with appropriate cell-lineage compartmentalization and an open and accessible luminal surface. Chemical gradients applied to the crypt-villus axis promoted the creation of a stem/progenitor-cell zone and supported cell migration along the crypt-villus axis. This new approach combining microengineered scaffolds, biophysical cues and chemical gradients to control the intestinal epithelium ex vivo can serve as a physiologically relevant mimic of the human small intestinal epithelium, and is broadly applicable to model other tissues that rely on gradients for physiological function.

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Section: Introductionmentioning

confidence: 99%

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium

et al. 2017

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“…Mesenchymal stem cells (MSCs) are multipotent cells that are isolated from many adult tissues such as adipose, bone marrow, muscle, and umbilical blood. MSCs can be differentiated into mesoderm lineages, namely, adipocytes, osteoblasts, and chondrocytes . Furthermore, it has been shown that MSCs have the potential to differentiate into cardiomyocytes, myocytes, hepatocytes, and neural cells.…”

Section: Introductionmentioning

confidence: 99%

A simple and highly efficient method for transduction of human adipose‐derived mesenchymal stem cells

Bolandi

Rad

Soudi

et al. 2018

J of Cellular Biochemistry

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Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into a wide range of cell types and provide a potential to transfer therapeutic protein in vivo, making them valuable candidates for gene therapy and cell therapy. However, using MSCs in in vivo is limited due to the low rate of transfection and transduction efficacy. Therefore, developing methods to efficiently transfer genes into MSCs would provide a number of opportunities for using them in the clinic. Here, we introduce a simple and robust method for efficient transduction of human adipose‐derived MSCs by modification under the culture condition of human embryonic kidney cells 293 (HEK293T) and MSCs. Moreover, as a transduction enhancer, polybrene was replaced with Lipofectamine, a cationic lipid. Therefore, we showed that transduction of primary cells can be increased efficiently by modifying the culture condition.

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“…Stem cells are the “master cells” of tissues and organs of the body, which undergo differentiation to serve mainly for restoration or replacement of worn out, injured, or diseased cells of various tissues and organs , such as heart, bone marrow, brain, muscle tissues, skin, and so on, and have been adapted for their regenerative roles . There are two major types of stem cell in mammals: embryonic stem cells (ESC) which are somatic cells capable of differentiating to become part of all cell types found in tissues and organs of the body such as heart, nervous, immune system, etc, therefore, have been termed is pluripotent . ESC originates from the inner mass cells of a blastocyst .…”

Section: Dimensional Stem Cell Culturementioning

confidence: 99%

Application of nanomaterials in three‐dimensional stem cell culture

Aziz

Pashaiasl

Khodadadi

et al. 2019

J of Cellular Biochemistry

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Petri dish cultured cells have for long provided scientists an aperture to understanding cell's behavior both in normal and disease states as well as in vitro and in vivo. But recent advances have brought to light how the architecture and composite nature of the immediate environment within which the cell is proliferated can profoundly influence its phenotypic features and functions, thus making obvious, limitations of the conventional two‐dimensional cell culture despite it cost effectiveness. Fortunately, the transition to three‐dimensional (3D) cell culture has occurred concurrently with expanded knowledge of nanoscience and materials, thereby lending significant impetus for innovative research. This review is focused on the application of nanoparticles in 3D stem cell breeding, recent trends and developments in medical sciences for improved drug delivery, and treatment approaches to some human diseases. We also reviewed prevailing challenges and concerns of nanotoxicity as it continues to impede and delay clinical applications as well the ongoing concerted and multidisciplinary efforts to overcome them.

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Stem Cells Applications in Regenerative Medicine and Disease Therapeutics

Cited by 450 publications

References 125 publications

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium

A simple and highly efficient method for transduction of human adipose‐derived mesenchymal stem cells

Application of nanomaterials in three‐dimensional stem cell culture

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