Cytokine-induced differentiation and proliferation of human T lymphocytesin vitro: Effects of interleukin 2 and interleukin 6

Смольникова, В. В.; Voznyuk, A. V.; Потапнев, М. П.

doi:10.1007/bf02434879

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…T cell and macrophage cytokines were measured ex vivo for each tissue collected from each treatment group. Cytokines with crucial roles in Th cell differentiation and clonal expansion or inflammation were selected for assessment: (1) immune cell production of IL-2, an important cytokine for development and differentiation of Th cells and proliferative responses required for clonal expansion (22); (2) IL-4 and IL-10, cytokines, which promote Th2 cell development and have anti-inflammatory functions; (3) IFN-γ and TNF-α, which promote Th1 cell development and which drive inflammation (23, 24). …”

Section: Introductionmentioning

confidence: 99%

Targeting Î±- and Î²-Adrenergic Receptors Differentially Shifts Th1, Th2, and Inflammatory Cytokine Profiles in Immune Organs to Attenuate Adjuvant Arthritis

et al. 2014

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The sympathetic nervous system (SNS) regulates host defense responses and restores homeostasis. SNS-immune regulation is altered in rheumatoid arthritis (RA) and rodent models of RA, characterized by nerve remodeling in immune organs and defective adrenergic receptor (AR) signaling to immune cell targets. The SNS typically promotes or suppresses inflammation via α- and β2-AR activation, respectively, and indirectly drives humoral immunity by blocking Th1 cytokine secretion. Here, we investigate how β2-AR stimulation and/or α-AR blockade at disease onset affects disease pathology and cytokine profiles in relevant immune organs from male Lewis rats with adjuvant-induced arthritis (AA). Rats challenged to induce AA were treated with terbutaline (TERB), a β2-AR agonist (600 μg/kg/day) and/or phentolamine (PHEN), an α-AR antagonist (5.0 mg/kg/day) or vehicle from disease onset through severe disease. We report that in spleen, mesenteric (MLN) and draining lymph node (DLN) cells, TERB reduces proliferation, an effect independent of IL-2. TERB also fails to shift T helper (Th) cytokines from a Th1 to Th2 profile in spleen and MLN (no effect on IFN-γ) and DLN (greater IFN-γ) cells. In splenocytes, TERB, PHEN, and co-treatment (PT) promotes an anti-inflammatory profile (greater IL-10) and lowers TNF-α (PT only). In DLN cells, drug treatments do not affect inflammatory profiles, except PT, which raised IL-10. In MLN cells, TERB or PHEN lowers MLN cell secretion of TNF-α or IL-10, respectively. Collectively, our findings indicate disrupted β2-AR, but not α-AR signaling in AA. Aberrant β2-AR signaling consequently derails the sympathetic regulation of lymphocyte expansion, Th cell differentiation, and inflammation in the spleen, DLNs and MLs that is required for immune system homeostasis. Importantly, this study provides potential mechanisms through which reestablished balance between α- and β2-AR function in the immune system ameliorates inflammation and joint destruction in AA.

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Section: Introductionmentioning

confidence: 99%

Targeting Î±- and Î²-Adrenergic Receptors Differentially Shifts Th1, Th2, and Inflammatory Cytokine Profiles in Immune Organs to Attenuate Adjuvant Arthritis

et al. 2014

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2-Aminopyridine—a label for bridging of oligosaccharides HPLC profiling and glycoarray printing

et al. 2007

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2-Aminopyridine derivatives of oligosaccharides (OS-AP) were printed onto microchips by two different ways. The first method is based on direct covalent insertion of OS-AP in polyacrylamide gel 3D chip. The second method is based on conversion of OS-AP into more reactive OS-aminoalditol followed by covalent printing onto NHS-activated glass slides. This approach extends the range of saccharides suitable for covalent printing due to availability of commercial OS-AP and easy high-performance liquid chromatography separation of glycoprotein N-chains in form of AP derivatives.

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Mesenchymal stromal cells promote or suppress the proliferation of T lymphocytes from cord blood and peripheral blood: the importance of low cell ratio and role of interleukin-6

et al. 2009

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Cytokine-induced differentiation and proliferation of human T lymphocytesin vitro: Effects of interleukin 2 and interleukin 6

Cited by 4 publications

References 12 publications

Targeting Î±- and Î²-Adrenergic Receptors Differentially Shifts Th1, Th2, and Inflammatory Cytokine Profiles in Immune Organs to Attenuate Adjuvant Arthritis

Targeting Î±- and Î²-Adrenergic Receptors Differentially Shifts Th1, Th2, and Inflammatory Cytokine Profiles in Immune Organs to Attenuate Adjuvant Arthritis

2-Aminopyridine—a label for bridging of oligosaccharides HPLC profiling and glycoarray printing

Mesenchymal stromal cells promote or suppress the proliferation of T lymphocytes from cord blood and peripheral blood: the importance of low cell ratio and role of interleukin-6

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