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Background: To distinguish between subgroups of patients with acute leukemia, the rate of spontaneous (culture-induced) apoptosis of leukemic cells was evaluated using five methods.Methods: Leukemic cells (cells) from the bone marrow of children with acute lymphoblastic leukemia (ALL, n ‫؍‬ 112) and acute myeloid leukemia (AML, n ‫؍‬ 30) were cultured for 20 h in vitro. The level of apoptosis was detected by fluorescent microscopy after staining with acridine orange (AO) or by flow cytometry after staining using PI, JC-1, the APO-BRDU kit, or the AnnexinV-FITC kit.Results: ALL cells were significantly more sensitive to spontaneous apoptosis versus AML cells, as was detected by all methods. The least sensitive technique was apoptosis detection by sub-G1-peak/PI-staining. No difference in the rate of apoptosis in cells was determined between T-and

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