Cytokine Dosage in Fresh and Preserved Human Amniotic Membrane

Pereira, Mário Genilhu Bomfim; Gomes, José Álvaro Pereira; Rizzo, Luiz Vicente; Cristovam, Priscila Cardoso; Silveira, Laila Christina

doi:10.1097/ico.0000000000000673

Cited by 20 publications

(14 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Commonly, the EAM is collected and prepared for deep‐freeze storage (−80°C). While it has been demonstrated that cryopreservation of the EAM can conserve the cellular viability of the tissue, 26 yet degradation of beneficial proteins associated with long‐term storage is also reported 27 . Commercially available dehydrated amniotic membrane offers the advantage of requiring no specialized cryo‐storage, and it has been reported to improve corneal ulcer healing in diabetic humans 28 ; however, in a recent study, dehydrated EAM did not improve healing for horses with experimentally induced superficial corneal ulcers 29 .…”

Section: Introductionmentioning

confidence: 99%

Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Silva

Gibson

Jeong

et al. 2021

Veterinary Ophthalmology

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Silva

Gibson

Jeong

et al. 2021

Veterinary Ophthalmology

View full text Add to dashboard Cite

show abstract

“…A 10-0 nylon suture is widely employed to secure an AM to the ocular surface; however, such a suture limits the use of AMs because of the various suture-related side-effects. 14 PROKERA was developed to address the problems caused by the use of sutures, 17 , 18 but its fixation ring interferes with the ability of the AM to attach tightly to the ocular surface, and a discrepancy in size between the conjunctival sac and the ring causes discomfort to patients. It would definitely be of value to develop a contact lens-shaped AM that fits the native cornea without a supporting device.…”

Section: Discussionmentioning

confidence: 99%

“…13 These effects are associated with various factors, such as epidermal growth factor (EGF); EGF receptor (EGFR); keratinocyte growth factor; tumor growth factor (TGF)-α, TGF-β1, and TGF-β2; vascular endothelial growth factor; insulin growth factor (IGF); hepatocyte growth factor; and tissue inhibitor of metalloproteinase 1 secreted by the AM. 12 , 14 Moreover, the composition of the AM basement membrane is very similar to that of the collagen of the cornea and conjunctiva and supports the growth of epithelial cells. 15 All of these advantages have deemed AM useful for corneal injury and ulcer treatment.…”

Section: Introductionmentioning

confidence: 89%

Development of Contact Lens-Shaped Crosslinked Amniotic Membranes for Sutureless Fixation in the Treatment of Ocular Surface Diseases

Huh

Hong

et al. 2020

Trans. Vis. Sci. Tech.

View full text Add to dashboard Cite

To develop a new method of manufacturing contact lens-shaped crosslinked amniotic membranes (AMs) using glutaraldehyde (GA) and dialdehyde starch (DAS) as crosslinking agents. Methods: Amniotic membranes were placed on a curved plastic mold and crosslinked with either 4.5% DAS or 1% GA, after which their physical properties and biological safety were evaluated. Results: The tensile strength of the GA-and DAS-crosslinked samples was much increased compared with that of normal AMs. Neither crosslinking process affected AM transparency. Although the GA-crosslinked AM showed better enzymatic resistance, its physiological structure was severely damaged after the crosslinking process. On the other hand, compared with the GA-crosslinked AM, the DAS-crosslinked AM showed higher growth factor concentrations and better biocompatibility, similar to normal AMs. In addition, the DAS-crosslinked AM was effective in the recovery of corneal epithelial wounds and was well maintained over 3 days without decentration or degradation on the ocular surface in human subjects. Conclusions: Contact lens-shaped AMs were successfully prepared with crosslinking agents. Crosslinking with DAS did not affect the structural properties or biological activity of the AMs, and the improved mechanical properties helped the AM to maintain its curved shape. This crosslinking method allowed us to transplant AMs into patients' eyes without sutures. Translational Relevance: Sutureless fixation of contact lens-shaped AMs would be very convenient and safe for the treatment of corneal surface disease.

show abstract

“…Compared to the control group from 1 to 14 days, the mice treated with AM exhibited increased efficacy but not as effective as AM-HEP@EGF. This may be because the content of growth factor on AM decreased after preparation process and long-time storage and led to unsatisfactory therapeutic effects (Pereira et al, 2016). Heparinization of AM can be an effective method to adsorb EGF and maintain the active of it.…”

Section: Discussionmentioning

confidence: 99%

“…The biological performances of AM are due to various growth factors from amniotic epithelial cells (Brodovsky et al, 2000;Sridhar et al, 2000;Joseph et al, 2001). However, growth factors will be gradually lost and insufficient and cannot maintain the activity after AM preparation process and long-time storage (Pereira et al, 2016). Usually, AM needs pretreatment before use.…”

Section: Introductionmentioning

confidence: 99%

Heparin-Modified Amniotic Membrane Combined With Growth Factors for Promoting Corneal Wound Healing After Alkali Burn

Zhao

Zuo

Zhong

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Ocular chemical burns are potentially blinding ocular injuries and require urgent management. Amniotic membrane (AM) transplantation is an effective surgical treatment, one of the reasons is because AM is a rich source of growth factors that can promote epithelialization and wound healing. However, growth factors will be gradually lost and insufficient after preparation process and long-time storage, leading to unsatisfactory therapeutic effects. Herein, we present a modified AM (AM-HEP) for the supplement and sustained release of growth factor by surface grafting heparin for treatment of ocular chemical burns. Heparin grafting rate and stability, microstructure, physical property, and sustained release of epithelial growth factor (EGF) of AM-HEP were characterized. Biocompatibility and ability to promote corneal epithelial cell growth and migration were evaluated and compared with a biological amnion, which is available on the market in vitro. The therapeutic effects of AM-HEP combined with EGF (AM-HEP@EGF) in vivo had been evaluated in a model of mouse corneal alkali burn. The results indicated that heparin was introduced into AM and maintain stability over 3 weeks at 37°C. The modification process of AM-HEP did not affect microstructure and physical property after comparing with non-modified AM. EGF could be combined quickly and effectively with AM-HEP; the sustained release could last for more than 14 days. AM-HEP@EGF could significantly promote corneal epithelial cell growth and migration, compared with non-modified AM and control group. Faster corneal epithelialization was observed with the transplantation of AM-HEP@EGF in vivo, compared with the untreated control group. The corneas in the AM-HEP@EGF group have less inflammation and were more transparent than those in the control group. The results from in vitro and in vivo experiments demonstrated that AM-HEP@EGF could significantly enhance the therapeutic effects. Taken together, AM-HEP@EGF is exhibited to be a potent clinical application in corneal alkali burns through accelerating corneal epithelial wound healing.

show abstract

Cytokine Dosage in Fresh and Preserved Human Amniotic Membrane

Cited by 20 publications

References 37 publications

Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Development of Contact Lens-Shaped Crosslinked Amniotic Membranes for Sutureless Fixation in the Treatment of Ocular Surface Diseases

Heparin-Modified Amniotic Membrane Combined With Growth Factors for Promoting Corneal Wound Healing After Alkali Burn

Contact Info

Product

Resources

About