Cytogenotoxic and biological evaluation of the aqueous extracts of Grewia lasiocarpa: An Allium cepa assay

Akwu, Nneka Augustina; Naidoo, Yougasphree; Singh, Moganavelli

doi:10.1016/j.sajb.2019.08.009

Cited by 23 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(Dias et al, 2007); interferência no trânsito intestinal (Marçal, Almeida & Carvalho, 2007) e na atividade da acetilcolinesterase, induzidos por compostos presentes em extratos vegetais, inibindo-a moderadamente (Mota et al, 2012). Efeitos na mitose em raízes de A. cepa foram constatados por diferentes autores (Bezerra & Oliveira, 2016;Bastos et al, 2017;Akwu et al, 2019;Rieder et al, 2019).…”

Section: Resultsunclassified

“…A literatura oferece estudos que revelam ações de vários fatores interferentes no processo da mitose, entre os quais se tem: atividade de compostos presentes em extratos vegetais verificáveis no teste com raízes de cebola (A. cepa) (Bezerra & Oliveira, 2016;Bastos et al, 2017;Akwu et al, 2019;Rieder et al, 2019); indução ou supressão da mitose por fatores físicos e químicos (De Felippe, 2018); estímulo da mitose, através do EGF (Fator de Crescimento Epitélico), agindo em várias linhagens celulares (Consolaro & Consolaro, 2010); impactação no fuso mitótico e segregação cromossômica, principalmente na fase G1 e no controle da transição da fase G1 para a fase S devido a alterações genéticas ou epigenéticas, em genes envolvidos na progressão do ciclo celular (Penna, 2017); danos celulares severos, podem culminar na morte celular, pelo processo apoptótico ou por necrose, que podem ser provocados por estresse oxidativo, anóxia, isquemia, substâncias tóxicas (Meneguelo, 2007); estabelecimento e controle do processo mitótico através das funções de enzimas proteínas quinases dependentes de ciclina-CdK (Carneiro & Junqueira, 2012); atuação de compostos químicos com propriedades farmacológicas afetando a mitose, tal como o agente tiabendazol (TBZ) [2-(4'-tiazolil)-1H-benzimidazol], derivado do bensimidazol, em que seus produtos antiparasitários afetam a mitose, o número de cromossomos, a função do fuso e as estruturas dos microtúbulos (L.P. Silva, 2004); interferência na mitose por agentes naturais (ex. : Hormônios) e patológicos (ex.…”

Section: O Processo Da Mitose E Fatores Interferentesunclassified

“…As plantas constituíram a base da medicina tradicional (Gurib-Fakim, 2006) e, as medicinais são usadas com segurança pela população quando há conhecimento e indicação adequada, acompanhada por profissionais da área, sendo importante o usuário saber dos riscos e benefícios envolvidos (Almeida, 2019;Rempel, Maciel, Bergmann, De Morás & Goettens, 2019). O uso de erva medicinal requer qualidade, segurança e eficácia; razão pela qual a avaliação científica exata deve ser previamente realizada para então endossar, ou não, sua aplicação em terapias com plantas (Akwu, Naidoo & Singh, 2019).…”

Section: Introductionunclassified

See 2 more Smart Citations

Potencial bioativo de ervas-de-passarinho: Efeito do extrato aquoso de folhas de Phoradendron piperoides (Kunt.) Trel. (Santalaceae) na mitose

Rieder

Rodrigues

Santos

et al. 2021

RSD

View full text Add to dashboard Cite

Ervas-de-passarinho medicinais são utilizadas também em câncer-terapias. Nesse trabalho avaliaram-se efeitos mitóticos do extrato aquoso de folhas do Phoradendron piperoides (Kunth) Trel.. O material vegetal foi coletado em Cáceres, Mato Grosso, Brasil, 2017. Aplicou-se o teste Allium cepa com três concentrações de extrato aquoso foliar (0, 6 e 18 g do pó de folhas/500 mL de água). As cebolas com raízes recém-emitidas ficaram imersas, na solução, por 24 horas (ação) e, por mais 24 horas em água (recuperação). Foram coletadas raízes para microscopia e contagem de células para cálculo dos índices mitóticos (IM%). O IM% na maior concentração foi menor que na intermediária (3,95% c < 16,80% b) e, estas duas, distinguiram-se do controle (60,40% a). A inibição da mitose acentuou-se com o aumento da concentração do extrato. Os IM%, no controle dos ensaios de ação e de recuperação estiveram similares (60,47%), mas maiores que nas demais concentrações; nas doses intermediária (16,80% B < 36,60% A), maior (3,95% B < 46,00% A) e na média geral (27,05% B < 47,71% A) foram menores (p < 0,05) na ação que na recuperação. Por fim evidencia-se que P. piperoides possui compostos bioativos, extraídos em água, inibidores da mitose, efeitos dose-dependente e parcialmente reversíveis na recuperação.

show abstract

Section: Resultsunclassified

Section: O Processo Da Mitose E Fatores Interferentesunclassified

Section: Introductionunclassified

See 1 more Smart Citation

Potencial bioativo de ervas-de-passarinho: Efeito do extrato aquoso de folhas de Phoradendron piperoides (Kunt.) Trel. (Santalaceae) na mitose

Rieder

Rodrigues

Santos

et al. 2021

RSD

View full text Add to dashboard Cite

show abstract

“…They were sun dried for 1 week in order to facilitate their growth when planted for the antigenotoxicity evaluation. The outer scales of the dried onions were removed and the primordial root ring was carefully trimmed to remove dried old roots (Akinboro et al, 2017;Akwu et al, 2019). The absolute Aloe vera gel extract (100.0%) was diluted with distilled water to prepare 6.25%, 12.5%, 25.0%, 50.0% doses.…”

Section: Allium Cepa Assaymentioning

confidence: 99%

Antigenotoxic potential of gel extract of Aloe vera against Sodium azide genotoxicity in Allium cepa cells

Akinboro

Jimoh

2021

JMHE

View full text Add to dashboard Cite

Nowadays, the increasing rate of human exposure to various kinds of environmental mutagens has necessitated the search for natural antimutagens /antigenotoxic agents in natural products. In this study, Aloe vera gel extract was tested for its possible antigenotoxicity following the Allium cepa assay. Ten onions (Allium cepa) per dose were grown for 48 and 72 hours on gel extract of A. vera at 6.25%, 12.5%, 25.0%, 50.0% and 100.0% in combination with sodium azide (0.05mg/ml) solution for microscopic and macroscopic evaluations, respectively. Distilled water and sodium azide were the negative and positive controls, respectively. The cell division in the root tips, and root growth in the exposed A. cepa were inhibited in a dose dependent manner by the mixture of A. vera and sodium azide. However, the mixture of absolute (100.0%) dose and sodium azide completely arrested cell division and induced a lower root length than that recorded for sodium azide alone. The genotoxicity of sodium azide was inversely reduced by the doses of A. vera except at 100.0%. These results show that gel extract of A. vera possesses strong antigenotoxic /antimutagenic potency at lower dose range of 6.25% to 25.0% in A. cepa cells, however, its higher doses above 50.0% to 100.0% could be severely toxic when being considered for suppression of environmental mutagens’ mutagenicity or genotoxicity. This suggests that gel extract of A. vera contains phytochemical(s) that can be useful in the development of anticancer drug.

show abstract

“…Medicinal plants, although used for their therapeutic properties, also exhibit dose dependent toxic effects. Allium cepa root tip meristematic cells assay easily determines dose dependent cytotoxicity and genotoxicity of medicinal plant extracts and plant-derived compounds [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

An evidence based efficacy and safety assessment of the ethnobiologicals against poisonous and non-poisonous bites used by the tribals of three westernmost districts of West Bengal, India: Anti-phospholipase A2 and genotoxic effects

et al. 2020

View full text Add to dashboard Cite

Introduction To explore the ethnobiological wisdom of the tribals of three western districts of West Bengal, India against poisonous and non-poisonous bites and stings, a quantitative approach was adopted. These age-old yet unexplored knowledge can be utilized in finding lead-molecules against poisonous and non-poisonous animal-bites. Further, an evidence-based approach is needed to assess the venom-neutralization ability of plants by experimental studies. Materials and methods During 2008–2009 and 2012–2017, 11 ethnomedicinal surveys were carried out to explore the use of medicinal flora and fauna via conducting open semi-structured interviews with 47 traditional healers (THs) or informants. The retrieved dataset was statistically evaluated using seven quantitative-indexes: use-value (UV), informants'-consensus-factor (ICF), fidelity-level (FL), relative-importance (RI), cultural importance-index (CI), index of agreement on remedies (IAR) and cultural agreement-index (CAI). Anti-phospholipaseA2 (PLA2) properties of selected plant extracts were also examined. In addition, the cytotoxicity and genotoxicity of the water extract of the plants showing high FL as well as significant PLA2 inhibitory potential were investigated using Allium cepa root tip assay. Results A total of 41 traditional-formulations (TFs) containing 40 plant species (of 39 genera from 28 families) and 3 animal species were prescribed by the THs. Fabaceae exhibited most number of medicinal plants. Piper nigrum (1.78) and Apis cerana indica and Crossopriza lyoni (both 0.21) exhibited the highest UV among the plants and the animals respectively. Stinging of centipede and dog/cat/hyena bite displayed highest ICF (1.00 each). Among the plants, the maximum RI (0.91) and CI (4.98) values were observed for Aristolochia indica. IAR (1.00) was recorded maximum for Achyranthes aspera, Gloriosa superba, Lycopodium cernuum, Smilax zeylanica and Streblus asper. Maximum CAI value was noted for Piper nigrum (5.5096). Among the animals, Apis cerana indica (0.31) and Crossopriza lyoni (1.52) displayed the highest RI and CI values respectively. Crossopriza lyoni (0.99) and Apis cerana indica (1.3871) exhibited maximum IAR and CAI values respectively. Plants showing higher FL exhibited higher anti-PLA2 activity via selective inhibition of human-group PLA2. In addition, Allium cepa root tip assay has indicated the safety and/or toxicity of the plant parts prescribed by the THs. Root water extracts of Aristolochia indica and Gloriosa superba exhibited significant genotoxicity and cytotoxicity. Conclusions Three western districts of West Bengal is the natural abode for many tribal and non-tribal communities. A noteworthy correlation was established between the plants used against poisonous-bites and their anti-PLA2 activity. A few plant parts used by the THs also exhibited high toxicity. Such alternative medical practices serve as the only option in these underprivileged and backward areas during medical-exigencies.

show abstract

Cytogenotoxic and biological evaluation of the aqueous extracts of Grewia lasiocarpa: An Allium cepa assay

Cited by 23 publications

References 33 publications

Potencial bioativo de ervas-de-passarinho: Efeito do extrato aquoso de folhas de Phoradendron piperoides (Kunt.) Trel. (Santalaceae) na mitose

Potencial bioativo de ervas-de-passarinho: Efeito do extrato aquoso de folhas de Phoradendron piperoides (Kunt.) Trel. (Santalaceae) na mitose

Antigenotoxic potential of gel extract of Aloe vera against Sodium azide genotoxicity in Allium cepa cells

An evidence based efficacy and safety assessment of the ethnobiologicals against poisonous and non-poisonous bites used by the tribals of three westernmost districts of West Bengal, India: Anti-phospholipase A2 and genotoxic effects

Contact Info

Product

Resources

About