Cytogenetics of uncleaved oocytes and arrested zygotes in IVF programs

Benkhalifa, Moncef; Ménézo, Yves; Janny, L.; Pouly, J ­L; Qumsiyeh, Mazin B.

doi:10.1007/bf02072536

Cited by 39 publications

(19 citation statements)

References 25 publications

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“…In contrast, Angell (1997) found only single chromatids in addition to, or replacing, whole chromosomes, and Verlinsky et al (1996b) observed chromatid malsegregation more frequently than chromosome nondisjunction. The remaining studies have reported only nondisjunction events (Martin et al, 1986;Veiga et al, 1987;Bongso et al, 1988;Djalali et al, 1988;Pellestor and Sèle, 1988;Papadopoulos et al, 1989;Macas et al, 1990;Tarín et al, 1991;Zenzes et al, 1992;Almeida and Bolton, 1994;Ma et al, 1994;Roberts and O'Neill, 1995;Benkhalifa et al, 1996). We found that the chromosomes more frequently involved in aneuploidy events were those of group D (45.5 %), according to other authors (Djalali et al, 1988;Macas et al, 1990;Zenzes et al, 1992;Kamiguchi et al, 1993).…”

Section: Discussionsupporting

confidence: 59%

Chromosome identification in human oocytes and polar bodies by spectral karyotyping

Márquez¹,

Cohen²,

Munné³

1998

Cytogenet Genome Res

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Sixty unfertilized human oocytes and two fresh polar bodies were karyotyped by spectral karyotyping (SKY). The oocytes were provided by 29 women ranging from 30 to 42 yr of age. The mean hybridization efficiency for oocytes was 95.2% (60/63). Nondisjunction of bivalent chromosomes (13.3%) and predivision of sister chromatids at meiosis I (3.3%) were unequivocally determined by analysis first with SKY and then fluorescence in situ hybridization. Four oocytes (6.7%) were hyperhaploid, six (10.0%) were hypohaploid, one (1.7%) showed balanced predivision, and another (1.7%) was diploid. No specific structural rearrangements were detected. This study demonstrates that the SKY technique can be used successfully as an alternative method of karyotyping second meiotic metaphase chromosomes from human oocytes and polar bodies in appropriate spreads.

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Section: Discussionsupporting

confidence: 59%

Chromosome identification in human oocytes and polar bodies by spectral karyotyping

Márquez¹,

Cohen²,

Munné³

1998

Cytogenet Genome Res

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“…In addition, prolonged culture has been suggested to allow the selection of chromosomally competent embryos, because embryos failing to develop more often show multiple aneuploidy [23,24]. However, the possible influences of an extended embryo culture to the blastocyst stage on the incidence of monozygotic multiples and the cancellation of embryo transfer may be of concern [25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Preferable correlation to blastocyst development and pregnancy rates with a new embryo grading system specific for day 3 embryos

Nomura

Iwase

Furui³

et al. 2006

J Assist Reprod Genet

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Purpose: To investigate the cleavage stage embryo quality by the correlation between the morphological features and blastocyst development rate to develop a new embryo grading system.Methods: A retrospective analysis, including 216 cycles of cleavage stage embryo transfer and 251 cycles of blastocyst transfer. The correlation with blastocyst development of the embryo cleavage stage, fragmentation and uniformity of blastomeres was evaluated.Results: There were significant differences in the blastocyst development rate between ≥7 cells and ≤6 cells (68.8% vs. 30.7%), <50% fragmentation and ≥50% fragmentation (51.9% vs. 25.7%), and evenly sized blastomeres and unevenly sized blastomeres (48.7% vs. 30.1%) on day 3. The new grading system defined by these 3 parameters showed a preferable correlation to the pregnancy rate.Conclusions: The new grading system specific for day 3 embryos is useful for the selection of good quality embryos and may improve the pregnancy rate.

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“…44 Ya pı lan ça lış ma lar da %23 ile %80 ara sın da kro mo zo mal ano ma li nin tes pit edil di ği gös te rilmiş tir. Bu ça lış ma da ise ano ma li li em bri yo la rın ora nı nın %80 ol du ğu gö rül müş ve so nuç lar li te ratür ile uyum lu bu lun muş tur.…”

Section: Medical Geneticsunclassified

Comparison of Aneuploidy Frequency to Sperm FISH and Sperm Apoptosis Results in Embryos That Lost the Vitality

Çankaya¹,

Ozkinay²,

Gündüz³

et al. 2011

Turkiye Klinikleri J Med Sci

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896Canlılığını Yitirmiş Embriyolarda Gözlenen Anöploidi Sıklığının Sperm-FISH ve Sperm-Apoptozis Sonuçları ile Karşılaştırılması Ö ÖZ ZE ET T A Am ma aç ç: : Em bri yo lar da gö rü len kro mo zom ano ma li ora nı nın spon tan abor tus lar da gö rü len de ğer le re gö re da ha yük sek ol du ğu bi lin mek te ve bu oran yak la şık %23 ile 80 ara sın da de ğiş mek te dir. Bu ça lış ma da ge li şi mi durmuş em bri yo lar da; 13,16,18,21 ve 22 no lu kro mo zom la ra ait sa yı ano ma li le ri nin araş tı rıl ma sı Kro mo zom ano ma li oran la rı ile ay nı sik lus ta bu lu nan em bri yo la rın can lı lık oran la rı ara sın da ki iliş ki nin gös -te ril me si, Ve ay nı sik lus ta el de edil miş sperm hüc re le rin de ki kro mo zom sa yı ano ma li le ri ile apop to zis ilş ki si nin araş tı -rıl ma sı amaç lan mış tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Ge li şi mi dur muş 20 em bri yo da 13,16,18,21 ve 22 no lu kro mo zom la rın sa yı sal ano ma li le ri FISH yön te mi ile ana liz edil di. Ay nı yön tem le em ri yo la rın ba ba la rın dan ay nı sik lus ta el de edi len sperm hüc rele rin de X,Y ve 18 no lu kro mo zom la rın sa yı ano ma li le ri ana liz edil di. Sperm hüc re le rin de apop to zis du ru mu nu değer len dir mek için TU NEL tes ti kul la nıl dı. B Bu ul l g gu u l la ar r: : Ge li şi mi dur muş em bri yo lar da ki kro mo zom sa yı ano ma li ora nı %80 ola rak bu lun muş tur. Em bri yo lar da gö rü len sa yı sal ano ma li ora nı ile em bri yo lar da ki can lı lık oran la rı ve sperm ler de ki top lam ano ma li ora nı ara sın da an lam lı iliş ki sap tan ma mış tır. Sperm apop to zi si ile em bri yo lar da gö rü len sa yı sal ano ma li oran la rı ara sın da iliş ki sap tan ma mış tır. Sperm ler de gö rü len kro mo zom ano ma li sa yı sı ile sper mi og ram so nuç la rı ara sın da an lam lı so nuç sap tan mış tır. S So o n nu uç ç: : Ge li şi mi dur muş em bri yo lar da 13,16,18,21 ve 22 no lu kro mo zom la ra ait sa yı sal ano ma li ora nı yük sek tir.Sperm ler de gö rü len sa yı sal kro mo zo mal ano ma li ve apop to zis sık lı ğı ile in ce le di ği miz em bri yo lar da gö rü len sa yı sal kro mo zo mal ano ma li ler ara sın da iliş ki bu lun mamış fa kat ba ba aday la rın dan el de edi len sper mi og ram so nuç la rı ile ge li şi mi dur muş em bri yo lar da gö rü len sa yı sal ano ma li ler ara sın da an lam lı iliş ki bu lun muş tur.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : Pre imp lan tas yon ta nı; in si tu hib ri di zas yon, flö re sans; blas to mer ler; anöp lo i di; in si tu nick-end la be ling A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Ra te of chro mo so mal ab nor ma lity which is se en in emb ryos is hig her than tho se in sponta ne o us aborts. This ra te va ri es from 23 to 83 per cent. In this study; in emb ryos with de ve lop ment ar rest, we ai med To in ves ti ga te the num ber ab nor ma li ti es in 13 rd , 16 th , 18 th , 21 st and 22 nd chro mo so mes, To show the cor re la ti on bet we en the chro mo so mal ab nor ma lity ra tes in emb ...

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Cytogenetics of uncleaved oocytes and arrested zygotes in IVF programs

Cited by 39 publications

References 25 publications

Chromosome identification in human oocytes and polar bodies by spectral karyotyping

Chromosome identification in human oocytes and polar bodies by spectral karyotyping

Preferable correlation to blastocyst development and pregnancy rates with a new embryo grading system specific for day 3 embryos

Comparison of Aneuploidy Frequency to Sperm FISH and Sperm Apoptosis Results in Embryos That Lost the Vitality

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