Cytogenetics ofLens EsculentaMoench

Bhattacharjee, S. K.

doi:10.1080/00087114.1953.10797436

Cited by 40 publications

(27 citation statements)

References 4 publications

(1 reference statement)

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“…The occurrence of lagging chromosomes may be due to abnormal spindle formation and the failure of chromosomal movement. According to Bhattacharjee (1953), accentric fragments or laggards may result in the formation of micronuclei at telophase II and ultimately in number and size of pollen grains resulting from pollen mother cell. Anaphasic bridges may be formed due to unequal exchange or dicentric chromosomes.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic Effects Induced by Methyl Methane Sulphonate in 2 Cultivars of Capsicum annuum L.

2011

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Summary Seeds of Capsicum annuum L. cv. Pusa jwala and G 4 were treated with varying concentrations of methyl methane sulphonate (MMS), and the effect of various concentrations of MMS on each stage of meiosis has been studied in M1 generation. Various types of meiotic abnormalities and reduction in chiasma frequency were observed in treated populations. However, MMS treatment were found to be more effective in inducing meiotic abnormalities and reduction in chiasma frequency in Pusa jwala as compared to G 4 . Moreover, a co-linear relationship between increasing concentrations of MMS and reduction in chiasma frequency was also observed. Taking the percentage of meiotic abnormalities and reduction in chiasma frequency as an index of sensitivity of genotype to MMS, Pusa jwala was found to be more sensitive than G 4 . Key words Capsicum annuum, Chiasma frequency, Meiotic aberrations, MMS.Mutagenesis is an important method of inducing variations in a number of plant characters from time to time, and has a great relevance for raising superior plant types in different crop plants. Mutagenesis is a potential tool for the selection of efficient mutagens and their treatment doses are prerequisite for obtaining the potential mutations.Cytological studies are necessary to obtain information regarding the effect of the mutagens and in elucidating the response of various genotypes to a particular mutagen. Chemical mutagens provide a very good tool for creating alterations in the genotypes and enhancing the variability of characters. Keeping in view the economic importance of C. annuum (2n=24), as a vegetable as well as a spice crop, the present study was under taken to asses the effect of MMS on 2 cultivars of C. annuum. Materials and methodsFresh and healthy seeds of C. annuum cv. Pusa jwala and G 4 , obtained from the Indian Agriculture Institute New Delhi, were presoaked in distilled water for 12 h under laboratory conditions and then treated with different concentrations of MMS i.e., 0.01%, 0.02%, 0.03%, 0.04% and 0.05% for 7 h. The controls were maintained separately. After each treatment seeds were thoroughly washed in tap water and sown in earthen pots to raise the M 1 generation. After 30 days of showing, the seedlings were transplanted into the field. At the flowering stage young flower buds were collected and fixed in freshly prepared Carnoy s fixative for 24 h and preserved in 70% ethanol to study the chromosomal behavior at different stages of meiosis in the pollen mother cells (PMCs) of both control and treated plants. Squashes were made in 2% acetocarmine and the data was recorded at each stage of meiosis.

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Section: Discussionmentioning

confidence: 99%

Genotoxic Effects Induced by Methyl Methane Sulphonate in 2 Cultivars of Capsicum annuum L.

2011

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“…The causes of bridges and laggard formation may also be due to interlocking of bivalents (Bhattacharjee 1953) or paracentric inversion (Sinha and Godward 1972) or delayed chiasma terminalization (Kumar and Tripathi 2007). Cytomixis was observed during early prophase, diakinesis and T-I involving two to three PMCs (Figs.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Meiotic Behavior in Eremurus himalaicus Baker (Liliaceae): A Rare Endemic Perennial from Kinnaur, Himachal Pradesh, India

Kumari

Saggoo

2016

CYTOLOGIA

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Summary Detailed meiotic studies have been carried out in 11 accessions of Eremurus himalaicus Baker belonging to family Liliaceae an endemic medicinal plant of the Northwestern Himalayas. E. himalaicus, due to its excessive exploitation for edible uses, is living under stress and has therefore been listed in the Red Data Book of Indian Plants as a rare species. Considering x=7 as the basic chromosome number for the species, E. himalacus revealed the diploid chromosome count of 2n=14. Out of 11 populations worked out cytologically, 3 populations of the species were normal in their meiotic behavior while the rest of the populations showed abnormal meiotic behavior. The phenomenon of cytomixis, presence of two to five nucleoli and various other meiotic abnormalities were observed in the form of unoriented bivalents, chromosome stickiness, late disjunction, laggards and bridges in eight populations. Earlier studies were limited to the counting of chromosome number, so these meiotic abnormalities were reported for the first time in the studied species. Further, the microsporogenesis was also abnormal leading to the formation of monad, dyads, triads and abnormal tetrads with the fusion among tetrads which ultimately leads to reduced pollen fertility.

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“…The laggards observed during the study might be due to delayed terminalisation, stickiness of chromosomal ends or because of failure of spindle fiber to bind on kinetochopre ). According to Bhattacharjee (1953), acentric fragments or laggards may result in the formation of micronuclei at telophase-II and ultimately variation in number and size of pollen grains resulting from a mother cell. Saylor and Smith (1966) suggested that the formation of chromatin bridges might be due to the failure of chiasmata in bivalent to be terminated and chromosome get stretched between the poles.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic effects of 5-bromouracil on cytomorphological characters of Cichorium intybus L.

Jafri¹,

Khan²,

Gulfishan³

2011

Afr. J. Biotechnol.

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This work was done to study the effect of base analogue 5-bromouracil (5-BU) on the medicinal herb, Cichorium intybus. 5-BU induced miss pairing during the DNA replication. The seeds of C. intybus were treated with different concentrations of 5-BU. Variations in some parameters such as seed germination, seedling survival, seedling height, pollen fertility, days to flowering, days to maturity, number of leaves per plant, plant height, and chromosome behavior were studied in M1 generation. A positive correlation between increasing concentrations of mutagen and various cytomorphological characters of C. intybus was observed.

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Cytogenetics ofLens EsculentaMoench

Cited by 40 publications

References 4 publications

Genotoxic Effects Induced by Methyl Methane Sulphonate in 2 Cultivars of <i>Capsicum annuum</i> L.

Genotoxic Effects Induced by Methyl Methane Sulphonate in 2 Cultivars of <i>Capsicum annuum</i> L.

Analysis of Meiotic Behavior in <i>Eremurus himalaicus</i> Baker (Liliaceae): A Rare Endemic Perennial from Kinnaur, Himachal Pradesh, India

Genotoxic effects of 5-bromouracil on cytomorphological characters of Cichorium intybus L.

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