Cytogenetic and Immunological Effects on Human Blood Cultures Resulting from Cold Pulsed Atmospheric Pressure Plasma Jet Exposure

Ahmed, M. M.; El-Aragi, Gamal M.; Elhadary, Abdel Monsef A.; Montaser, Sherien Abdelwahab

doi:10.1615/plasmamed.2013007992

Cited by 4 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the period time of 80 sec. was the most effective and save dose for attendant with the advice of Ahmed et al [59][60][61].…”

Section: Discussionmentioning

confidence: 92%

Assessment of Cytogenetic Instability and Gene Transcription of Chronic Myelogenous Leukemia Cells Exposed to Non-thermal Plasma

AMA¹

2015

J Cytol Histol

View full text Add to dashboard Cite

Background: This study investigates the effect of Atmospheric Pressure Plasma Jet (APPJ) on cytogenetic instability and transcription of genes associated with propagation of chronic myelogenous leukemia (CML). Material and methods: Blood samples were collected from six patients with CML and compared with control group. The first three cases were in accelerated phase (AP-CML) resistant to imatinib and nilotinib drugs. The others partially responded and returned to chronic phase (CP-CML). Triple blood cultures for each case were exposed to single dose of plasma, with treatment times of 40, 80, and 120 sec. Mitotic phases, indices and abnormalities, as well transcription levels of P53, caspase-3 and BcL-2 genes were detected before and after plasma jet exposure. Results and conclusion: The data showed that the exposure to APPJ caused remission of the mitotic indices and give a significant increase in the arresting of chromosome instabilities by induction of different mitotic checkpoints and express of sticky and condensed chromosomes which ended by apoptosis and necrosis processes. Additionally, the same events detected for the transcriptional levels of P53, caspase-3 and Bcl-2 genes.

show abstract

“…In addition, the period time of 80 sec. was the most effective and save dose for attendant with the advice of Ahmed et al [59][60][61].…”

Section: Discussionmentioning

confidence: 92%

Assessment of Cytogenetic Instability and Gene Transcription of Chronic Myelogenous Leukemia Cells Exposed to Non-thermal Plasma

AMA¹

2015

J Cytol Histol

View full text Add to dashboard Cite

show abstract

“…[22][23][24][25] CAPPJ and gliding arc plasma exposure can be utilized to repair tissues, treat some diseases, and treat tumors, especially drug-resistant forms, according to evidence from several studies on various types of plasma and their effects on both healthy and malignant cells. [26,27] The most significant improvements in the halting of chromosomal instabilities were achieved by stimulation of several mitotic checkpoints, followed by apoptosis and necrosis processes, which were plasma's most effective roles. The same events were also found to increase immunological responses (interleukins and TNF-α) and the transcription of anti-tumor genes (P53, caspase-3, and Bcl2).…”

Section: Bl-d4 Bl-d3 Bl-d2 Bl-d1 Control Groupsmentioning

confidence: 99%

Another Concept of Cancer Interpretation in View of the Interaction between Plasma Radiation and DNA

Ahmed¹,

Montaser²,

Elhadary³

et al. 2022

Clin Cancer Investig J

View full text Add to dashboard Cite

Several studies included our preceding works on different types and doses of plasma sources on both normal and cancerous cells. All previous research tried to discuss and interpret cancer treatment concepts given plasma and cell interaction. Plasma has an effective role and specification character on cancerous cells outcome via many investigations (genetically, immunologically, and biochemically measurements) lead us to numerous paths of different theories which may create a new approach for considerations. This study was designed using a plasma jet powered by a Tesla coil used for generating Cold Atmospheric Pressure Plasma Jet (CAPPJ) from dielectric barrier discharge. The subject samples were categorized into three groups, the first was the cancer cell line. The second was normal blood samples whereas, the third one was non-exposed blood cells cultivated in CAPPJ-exposed cultures. The Cytokinesis Blocked Micronucleus Test (CBMN), a cytome assay, the protein expression of the P53 and Bcl2 genes, the interleukins (IL-1β, IL-6, and IL-10), and tumor necrosis factor (TNF-α) were the variables used in the current investigation. Results indicated that the direct interaction between cells and CAPPJ is more efficient than cells cultivated in CAPPJ-exposed cultures. Cell viability and protein expression levels of Bcl2 and P53 genes in CAPPJ irradiated Breast Cancer Cell lines (BCC) were remarkably valuable. CAPPJ affects cells via not only free radicals and enhancement of several important pathways but may be via direct interaction with DNA.

show abstract

“…The duration of 80 seconds was the most effective and safe dose of APPJ treatment. 43 Moreover, the presence of a low incidence of binucleated cells for CML in CBMN assay does not mean a low nuclear division index; it does, however, mean that control and governance of cytochalasin B in mitotic division is very low (high division rate). Therefore, for CML cells, a high ratio of binucleated cells is expressed and observed only after exposure to the APPJ.…”

Section: Figmentioning

confidence: 99%

Promising Trial for Treatment of Chronic Myelogenous Leukemia Using Plasma Technology

et al. 2013

Self Cite

View full text Add to dashboard Cite

This article presents a trial of the treatment of chronic-phase (CP) and accelerated-phase (AP) chronic myelogenous leukemia (CML) that is resistant or only partially responded to chemotherapy. Blood samples of 6 cases diagnosed with CML were studied and compared with a control group. The first 3 cases were AP CML resistant to imatinib and nilotinib. The other 3 partially responded to the chemotherapy and returned to CP CML. Triple blood cultures for each case were exposed to a cold, pulsed, atmospheric pressure plasma jet for different durations (40, 80, and 120 seconds). Hematological, cytogenetic, and biochemical investigations were done before and after plasma jet exposure. The results showed an increase in necrotic and apoptotic cell counts and a decrease in the number of characteristic nucleoplasmic bridges (multinucleated threadlike shape). Concentrations of transforming growth factor-β1 and arginase decreased in the CML blood samples after exposure to plasma jet.This type of nonthermal plasma can kill cancer cells and prevent the cells from dividing, especially for the 80-second duration.

show abstract

Cytogenetic and Immunological Effects on Human Blood Cultures Resulting from Cold Pulsed Atmospheric Pressure Plasma Jet Exposure

Cited by 4 publications

References 27 publications

Assessment of Cytogenetic Instability and Gene Transcription of Chronic Myelogenous Leukemia Cells Exposed to Non-thermal Plasma

Assessment of Cytogenetic Instability and Gene Transcription of Chronic Myelogenous Leukemia Cells Exposed to Non-thermal Plasma

Another Concept of Cancer Interpretation in View of the Interaction between Plasma Radiation and DNA

Promising Trial for Treatment of Chronic Myelogenous Leukemia Using Plasma Technology

Contact Info

Product

Resources

About