Cytocompatibility of <scp>HEMA</scp>–free resin–based luting cements according to application protocols on dentine surfaces

Soares, Diana Gabriela; Brito, Carolina Arouca Gomes de; Silva, R. H. B. Tavares da; Ribeiro, Ana Paula D.; Hebling, Josimeri; Costa, Carlos Alberto de Souza

doi:10.1111/iej.12479

Cited by 17 publications

(21 citation statements)

References 47 publications

(69 reference statements)

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“…Dentin discs (0.4 mm thick; n=56) were obtained from healthy human third molars (Process #32272114.9.0000.5188; Ethics Committee in Research of the Health Sciences Center of the Federal University of Paraíba, Brazil), as described in detail by Soares et al (11). The discs were ground wet with 400-and 600-grit abrasive papers (Carborundum Abrasives, Recife, PE, Brazil) to a set thickness at 0.4 mm, measured by digital caliper (Model 500-144B; Mitutoyo South America Ltda., Suzano, SP, Brazil).…”

Section: Preparation Of Dentin Discsmentioning

confidence: 99%

“…The discs were ground wet with 400-and 600-grit abrasive papers (Carborundum Abrasives, Recife, PE, Brazil) to a set thickness at 0.4 mm, measured by digital caliper (Model 500-144B; Mitutoyo South America Ltda., Suzano, SP, Brazil). Then, a 0.5 mol/L EDTA solution was applied to each surface for 60 s to remove the smear layer, followed by washing in deionized water for the same period (11). The discs were adapted to a filtration chamber, and a cannula was connected to a 1.80-m column of water through a 0.5-mm-diameter polyethylene tube.…”

Section: Preparation Of Dentin Discsmentioning

confidence: 99%

“…After hydraulic conductance analysis, the discs were randomly assigned to the experimental groups in such a way that the statistical analysis of the hydraulic conductance values presented similar dentin permeability among groups (one-way ANOVA, p>0.05). This procedure was carried out to standardize the permeability of the discs distributed among groups, since dentin hydraulic conductance plays a role in the diffusion of resin monomers through dentin (4,11).…”

Section: Preparation Of Dentin Discsmentioning

confidence: 99%

“…The artificial pulp chamber (APC; Fig. 1) employed in this investigation has also been used by other researchers (4,11). Each dentin disc was individually placed in the upper compartment, between two silicon rings (Rodimar Rolamentos Ltda, Araraquara, SP, Brazil), with the pulp surface facing down and the occlusal surface facing up.…”

Section: Artificial Pulp Chamber (Apc)mentioning

confidence: 99%

“…All discs had their hydraulic conductance measured to standardize the permeability of this tubular substrate for all groups (4,11). This method was used to simulate clinical situation on laboratory condition.…”

Section: Properties Of Universal Adhesive Systemsmentioning

confidence: 99%

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Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

et al. 2018

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This study evaluated application protocol (etch-and-rinse/ER and self-etching/SE) and dentin wettability (wet and dry) on microtensile bond strength (mTBS) and transdentinal cytotoxicity of Scotchbond TM Universal (SU) adhesive system. The mTBS values and fracture mode were registered 24 h after adhesive system application and resin composite block build-up (n=5). For analysis of transdentinal cytotoxicity, odontoblast-like MDPC-23 cells were seeded on pulpal surface of dentin discs (0.4 mm thick) adapted to artificial pulp chambers (n=8). The adhesive system was applied to occlusal surface, followed by 24-h incubation time. Cell viability (Alamar Blue) and morphology (SEM) were assessed. Adper Single Bond 2 and Clearfil SE Bond were used as positive controls of the ER and SE application protocols, respectively. No treatment was performed on negative control (NC) group. Data were analyzed by ANOVA and Tukey's tests (α=5%). Higher mTBS values were found for ER mode in comparison with SE protocol (p<0.05). Dentin wettability had no effect on bond strength of SU in both the ER and SE techniques (p>0.05). Most fractures involved hybrid layer and/or adhesive layer. Neither variable prevented the intense toxic effects of adhesive systems on MDPC-23 cultured cells, since intense reduction in cell viability (±88%) and severe alterations in cell morphology were observed for all groups compared to NC, with no differences among them (p>0.05). Therefore, it was concluded that application of SU following the ER protocol had better adhesive performance. However, this adhesive system featured intense transdentinal cytotoxicity to pulp cells, regardless of application protocol and dentin wettability.

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Section: Preparation Of Dentin Discsmentioning

confidence: 99%

Section: Preparation Of Dentin Discsmentioning

confidence: 99%

Section: Preparation Of Dentin Discsmentioning

confidence: 99%

Section: Artificial Pulp Chamber (Apc)mentioning

confidence: 99%

Section: Properties Of Universal Adhesive Systemsmentioning

confidence: 99%

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Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

et al. 2018

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Effect of various polymerization protocols on the cytotoxicity of conventional and self-adhesive resin-based luting cements

et al. 2019

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OBJECTIVES This study evaluated the cytotoxicity of resin-based luting cements on fibroblast cells using different polymerization protocols. MATERIALS AND METHODS Two conventional dual-polymerized (RelyX ARC, VariolinkN) and two self-adhesive resin cements (RelyX Unicem, Multilink Speed) specimens were polymerized using four different polymerization protocols: (a) photopolymerization with direct light application, (b) photo-polymerization over ceramic and (c) resin nanoceramic discs and (d) auto-polymerization. The specimens were then assigned to four groups to test cytotoxicity at 0, 1, 2 and 7 preincubation days (n = 5). MTT test was performed using NIH/3T3 fibroblast cells. Data were analysed using three-and one-way ANOVA. Multiple comparisons were made using Bonferroni post hoc test (p < 0.05). RESULTS The highest cytotoxic values were recorded at day 2 for conventional resin cements and at day 0 for self-adhesive resin cements. Self-adhesive resin cements showed the most cytotoxic effect at the second day, while conventional resin cements presented immediate cytotoxicity. Auto-polymerized resin specimens and especially Multilink Speed demonstrated the most cytotoxic effect regardless of the preincubation time. Cytotoxicity of cements tested reached the lowest level at day 7. Interposition of ceramic or nano-ceramic restorative material did not significantly affect the cytotoxicity of tested luting cements (p > 0.05). CONCLUSIONS Cytotoxicity of dual-polymerized resin cements was material-dependent and decreased gradually up to 7 days. Photo-polymerization plays an important role in reducing the cytotoxic effects. CLINICAL RELEVANCE When luting ceramic or resin nano-ceramic restorations of which thickness does not exceed 2 mm, the level of cytotoxicity with the tested materials is not significant. Luting of restorative materials that do not allow for light transmission such as metal-fused porcelain, clinicians should be cautious in the use of dual-polymerized conventional resin cements as only auto-polymerization of resin cements takes place under such materials.

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Differential cytotoxic effects on odontoblastic cells induced by self-adhesive resin cements as a function of the activation protocol

et al. 2017

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Cytocompatibility of HEMA–free resin–based luting cements according to application protocols on dentine surfaces

Abstract: HEMA-free Rely X ARC cement caused greater toxicity to odontoblast-like MDPC-23 cells than did Rely X Unicem cement when both resin-based luting materials were applied to dentine as recommended by the manufacturer.

Cited by 17 publications

References 47 publications

Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

Effect of various polymerization protocols on the cytotoxicity of conventional and self-adhesive resin-based luting cements

Differential cytotoxic effects on odontoblastic cells induced by self-adhesive resin cements as a function of the activation protocol

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