Cytochrome P450 Nomenclature, 2004

Nelson, David R.

doi:10.1385/1-59259-998-2:1

Cited by 129 publications

(148 citation statements)

References 11 publications

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“…Cytochromes P450 comprise a superfamily of heme enzymes with myriad biological functions, including key roles in drug metabolism, breakdown of xenobiotics, and steroid and secondary metabolite biosynthesis [ 18]. More than 4,500 sequences of this ubiquitous enzyme are known [ 19]. Members of the artificial family of chimeric P450s reported here differ from any known protein by up to 109 amino acids, yet most retain significant catalytic activity.…”

Section: Introductionmentioning

confidence: 99%

Structure-Guided Recombination Creates an Artificial Family of Cytochromes P450

et al. 2006

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Creating artificial protein families affords new opportunities to explore the determinants of structure and biological function free from many of the constraints of natural selection. We have created an artificial family comprising ˜3,000 P450 heme proteins that correctly fold and incorporate a heme cofactor by recombining three cytochromes P450 at seven crossover locations chosen to minimize structural disruption. Members of this protein family differ from any known sequence at an average of 72 and by as many as 109 amino acids. Most (>73%) of the properly folded chimeric P450 heme proteins are catalytically active peroxygenases; some are more thermostable than the parent proteins. A multiple sequence alignment of 955 chimeras, including both folded and not, is a valuable resource for sequence-structure-function studies. Logistic regression analysis of the multiple sequence alignment identifies key structural contributions to cytochrome P450 heme incorporation and peroxygenase activity and suggests possible structural differences between parents CYP102A1 and CYP102A2.

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Section: Introductionmentioning

confidence: 99%

Structure-Guided Recombination Creates an Artificial Family of Cytochromes P450

et al. 2006

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“…While versatile and rapidly changing, all the protostome CYP genes identified to date can be assigned to one of four clans: CYP2, CYP3, CYP4 and the mitochondrial CYP clan [9]. While clan nomenclature is inferred by phylogeny [10], members of each clan can have various roles. The CYP4 clan in insects for instance, includes members associated with pheromone synthesis and breakdown [11], as well as cuticle hardening [12].…”

Section: Conserved Arthropod Cyp450 Orthologs: the Halloween Genesmentioning

confidence: 99%

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Ventura

Bose

Fitzgibbon

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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, CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development, Journal of Steroid Biochemistry and Molecular Biologyhttp://dx.doi.org/10. 1016/j.jsbmb.2017.04.007 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

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“…More than 2000 plant P450 sequences are listed in the P450 database [Nelson, 2006;Nelson et al, 2004]. Some of these P450s are involved in secondary metabolism and in the metabolism of xenobiotics, including pesticides, but molecular-level information on the involvement of plant P450s in xenobiotic metabolism is limited.…”

Section: Use Of Animal P450smentioning

confidence: 99%

Transgenic Rice Plants Expressing Human P450 Genes Involved in Xenobiotic Metabolism for Phytoremediation

et al. 2008

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Phytoremediation is the use of plants to remove xenobiotic compounds from the environment. Plants have the inherent ability to detoxify xenobiotic pollutants, but they are generally poor at degrading them. The introduction of genes involved in xenobiotic degradation is aimed at enhancing plants’ potential further. Rice (Oryza sativa) is a good candidate for this purpose and has been transformed with genes encoding cytochrome P450 monooxygenases CYP1A1, CYP2B6, and CYP2C19. The transgenic plants were more tolerant to various herbicides than nontransgenic Nipponbare rice plants, owing to enhanced metabolism by the introduced P450 enzymes. Transgenic plants were able to remove atrazine and metolachlor from soil. Field testing and risk assessment are very important for developing transgenic plants for phytoremediation. Transgenic rice plants should become useful as herbicide-tolerant crops and for phytoremediation of xenobiotic pollutants in future.

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Cytochrome P450 Nomenclature, 2004

Cited by 129 publications

References 11 publications

Structure-Guided Recombination Creates an Artificial Family of Cytochromes P450

Structure-Guided Recombination Creates an Artificial Family of Cytochromes P450

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Transgenic Rice Plants Expressing Human P450 Genes Involved in Xenobiotic Metabolism for Phytoremediation

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