Transgenic Rice Plants Expressing Human P450 Genes Involved in Xenobiotic Metabolism for Phytoremediation

Kawahigashi, Hiroyuki; Hirose, Sakiko; Ohkawa, Hideo; Ohkawa, Yasunobu

doi:10.1159/000121332

Cited by 39 publications

(11 citation statements)

References 82 publications

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“…Up-regulation of Cyt450 gene family during heavy metal stress has been reported earlier, using microarray experiments, indicating their role in heavy metal detoxification [ 16 , 27 ]. Kawahigashi et al, [ 28 ] reported transgenic rice transformed with genes encoding human cytochrome P450 monooxygenases CYP1A1, CYP2B6, and CYP2C19 were more tolerant to various herbicides than nontransgenic Nipponbare rice, owing to enhanced metabolism by the introduced P450 enzymes. However, one of the big challenges is to define the range of functions for individual P450 proteins that show altered expression during Cr-stress.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic and metabolomic shifts in rice roots in response to Cr (VI) stress

et al. 2010

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BackgroundWidespread use of chromium (Cr) contaminated fields due to careless and inappropriate management practices of effluent discharge, mostly from industries related to metallurgy, electroplating, production of paints and pigments, tanning, and wood preservation elevates its concentration in surface soil and eventually into rice plants and grains. In spite of many previous studies having been conducted on the effects of chromium stress, the precise molecular mechanisms related to both the effects of chromium phytotoxicity, the defense reactions of plants against chromium exposure as well as translocation and accumulation in rice remain poorly understood.ResultsDetailed analysis of genome-wide transcriptome profiling in rice root is reported here, following Cr-plant interaction. Such studies are important for the identification of genes responsible for tolerance, accumulation and defense response in plants with respect to Cr stress. Rice root metabolome analysis was also carried out to relate differential transcriptome data to biological processes affected by Cr (VI) stress in rice. To check whether the Cr-specific motifs were indeed significantly over represented in the promoter regions of Cr-responsive genes, occurrence of these motifs in whole genome sequence was carried out. In the background of whole genome, the lift value for these 14 and 13 motifs was significantly high in the test dataset. Though no functional role has been assigned to any of the motifs, but all of these are present as promoter motifs in the Database of orthologus promoters.ConclusionThese findings clearly suggest that a complex network of regulatory pathways modulates Cr-response of rice. The integrated matrix of both transcriptome and metabolome data after suitable normalization and initial calculations provided us a visual picture of the correlations between components. Predominance of different motifs in the subsets of genes suggests the involvement of motif-specific transcription modulating proteins in Cr stress response of rice.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic and metabolomic shifts in rice roots in response to Cr (VI) stress

et al. 2010

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“…Horizontal gene transfer (HGT) in planta is likely to be widespread, as studies in pea with Pseudomonas endophytes harboring the plasmids Pwwo and pNAH7 also showed high rates of transfer into a range of autochthonous endophytes (Ryan et al 2007). Phytoremediation of herbicides has been enhanced by using transgenic plants expressing GST (Karavangeli et al 2005) or cytochrome P450 genes (Kawahigashi et al 2006).…”

Section: Remediation With Genetically Modified Endophytes and Plantsmentioning

confidence: 99%

Secondary plant metabolites and root exudates: guiding tools for polychlorinated biphenyl biodegradation

Panwar

Jha

2014

Int. J. Environ. Sci. Technol.

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Synthetic organic compounds are hallmark of modern society. They are ubiquitous ranging from home, workplace to agriculture industry, which leads to their nonjudicious dispensing into environment. Unfortunately most of them, especially polychlorinated biphenyls (PCBs), are deemed as persistent organic pollutants posing serious health risks to human. Hence, there is an alarming need of phasing out these chemicals and remediating contaminated sites in eco-friendly manner. Phytoremediation has emerged as a highly promising approach which capitalizes on plants and their associated microorganisms for removal of pollutants from targeted sites. Plant root exudations and secondary metabolites efficiently orchestrate selective recruitment of potential PCB-degrading microbial consortia within the rhizosphere and inside plant tissues. Structural analogy between organic contaminants and secondary plant metabolites (SPMEs) renders possible uptake and subsequent degradation of pollutants by microorganisms. Present review is focused on potential role of plant root exudates and SPMEs in structuring and orchestrating remediation of PCBs within rhizosphere and inside plant tissues. Also, recent developments in tools and techniques to study remediation of organic contaminants with special reference to PCBs are addressed.

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“…In the past decade CYP1A1, CYP2B6, CYP2B22, CYP2C9, CYP2C19, CYP2C49, CYP2E1, CYP71A1, CYP76B1, and CYP105A1 have been introduced in various plants, such as rice and tobacco, for phytoremediation of numerous herbicides, petrochemicals, and industrial contaminants [ 31 – 34 ]. For example, transgenic rice ( Oryza sativa ) plants that harbor CYP1A1, CYP2B6, and CYP2C19 genes were more tolerant to several herbicides than non-transgenic plants [ 32 – 33 ]. The transgenic rice plants, as a result of the CYP activity, showed enhanced herbicide metabolism and was able to remove atrazine, norflurazon, metolachlor, and sulfonylurea from the soil.…”

Section: Cytochrome P450-mediated Phytoremediationmentioning

confidence: 99%

Cytochrome P450-Mediated Phytoremediation using Transgenic Plants: A Need for Engineered Cytochrome P450 Enzymes

Kumar¹,

Jin²,

Weemhoff³

2012

J Pet Environ Biotechnol

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There is an increasing demand for versatile and ubiquitous Cytochrome P450 (CYP) biocatalysts for biotechnology, medicine, and bioremediation. In the last decade there has been an increase in realization of the power of CYP biocatalysts for detoxification of soil and water contaminants using transgenic plants. However, the major limitations of mammalian CYP enzymes are that they require CYP reductase (CPR) for their activity, and they show relatively low activity, stability, and expression. On the other hand, bacterial CYP enzymes show limited substrate diversity and usually do not metabolize herbicides and industrial contaminants. Therefore, there has been a considerable interest for biotechnological industries and the scientific community to design CYP enzymes to improve their catalytic efficiency, stability, expression, substrate diversity, and the suitability of P450-CPR fusion enzymes. Engineered CYP enzymes have potential for transgenic plants-mediated phytoremediation of herbicides and environmental contaminants. In this review we discuss: 1) the role of CYP enzymes in phytoremediation using transgenic plants, 2) problems associated with wild-type CYP enzymes in phytoremediation, and 3) examples of engineered CYP enzymes and their potential role in transgenic plant-mediated phytoremediation.

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Transgenic Rice Plants Expressing Human P450 Genes Involved in Xenobiotic Metabolism for Phytoremediation

Cited by 39 publications

References 82 publications

Transcriptomic and metabolomic shifts in rice roots in response to Cr (VI) stress

Transcriptomic and metabolomic shifts in rice roots in response to Cr (VI) stress

Secondary plant metabolites and root exudates: guiding tools for polychlorinated biphenyl biodegradation

Cytochrome P450-Mediated Phytoremediation using Transgenic Plants: A Need for Engineered Cytochrome P450 Enzymes

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