Cytidine deamination assay to differentiate Staphylococcus aureus from other staphylococci

Abstract

Aims: Adoption of the property of cytidine (cytosine‐β‐d‐riboside) deamination in staphylococci to distinguish Staphylococcus aureus from other staphylococci. Methods and Results: A total of 560 staphylococcal strains were examined. The test demonstrated a sensitivity of 97·1% and a specificity of 98·8%. Of the 249 S. aureus strains (115 oxacillin‐resistant) 58 strains were coagulase‐negative S. aureus and another 16 strains were clumping factor‐negative S. aureus. The 74 deficient S. aureus strains were identified by 16S rRNA gene sequencing and further investigated by spa typing and 13 spa types were found. Conclusions: The cytidine deaminase test (CDT) is useful especially for distinguishing coagulase‐ and clumping factor‐negative S. aureus from other staphylococci and the results correlated well with 16S rRNA sequencing and the polymerase chain reaction (PCR) amplification of the nuc gene. Significance and Impact of the Study: Cytidine deamination assay differentiates S. aureus from other staphylococci. This method is fast (6 h) and reliable in distinguishing between non‐S. aureus and the defective (coagulase‐negative, clumping factor‐negative) S. aureus isolates which could have major consequences for therapy.

Distribution of the Most Prevalent Spa Types among Clinical Isolates of Methicillin-Resistant and -Susceptible Staphylococcus aureus around the World: A Review

“…Out of the remaining articles, 253 fulfilled our inclusion criteria (Shopsin et al, 1999; Graille et al, 2000; Fey et al, 2003; Arakere et al, 2005; Denis et al, 2005; Ko et al, 2005; Aires-de-Sousa et al, 2006, 2008; Deplano et al, 2006; Durand et al, 2006; Ferry et al, 2006, 2010; Fossum and Bukholm, 2006; Jury et al, 2006; Kuhn et al, 2006; Mellmann et al, 2006, 2008; Montesinos et al, 2006; Ruppitsch et al, 2006, 2007; Sabat et al, 2006; Cai et al, 2007, 2009; Conceicao et al, 2007; Cookson et al, 2007; Ellington et al, 2007a,b; Ghebremedhin et al, 2007; Hallin et al, 2007, 2008; Krasuski et al, 2007; Matussek et al, 2007; Otter et al, 2007; Tristan et al, 2007; Van Loo et al, 2007; Vourli et al, 2007; Werbick et al, 2007; Witte et al, 2007; von Eiff et al, 2007; Bartels et al, 2008, 2013, 2014; Chaberny et al, 2008; Chmelnitsky et al, 2008; Gardella et al, 2008; Fenner et al, 2008a,b; Golding et al, 2008, 2011; Ho et al, 2008a,b, 2012, 2016, 2017; Jappe et al, 2008; Karynski et al, 2008; Larsen et al, 2008, 2009; Nulens et al, 2008; Pérez-Vázquez et al, 2008; Strommenger et al, 2008; Vainio et al, 2008, 2011; Zhang et al, 2008, 2009; Alp et al, 2009; Argudín et al, 2009, 2011; Atkinson et al, 2009; Bekkhoucha et al, 2009; Chen et al, 2009; Chen H.-J. et al, 2010; Chen L. et al, 2010; Chen et al, 20...…”

Metabolomics analysis of okara probiotic beverages fermented with Lactobacillus gasseri and Limosilactobacillus fermentum by LC-QTOF-MS/MS

“…Cytidine is formed when cytosine is attached to a ribose ring. Cytidine can be deaminated into uridine which is then cleaved to form uracil ( Krasuski, Michnowska-Swincow, & Jarzembowski, 2007 ). Conjugated forms of uracil were detected in both PCC- and LAC-fermented samples, suggesting that pyrimidine metabolism had led to the production of uracil, though the conjugated form of uracil would likely be less bioavailable.…”