Totally, 47 strains of S. aureus and 578 coagulase negative staphylococci were detected in samples from raw ewe milk. The 35 out 47 isolates of S. aureus from ewe milk were positive for the presence of staphylococcal enterotoxin genes: sea(4 %), sec (48 %) a sed (48 %). Staphylococcus epidermis (33.04%), Staphylococcus caprae (21.28%) were more prevalent. Staphylococcus chromogenes (7.44 %), Staphylococcus hominis (7.09%), Staphylococcus xylosus (6,92 %), a Staphylococcus warneri (6.40 %) were isolated also in ewes milk. Staphylococcus haemolyticus (3.11 %), Staphylococcus capitis (2.94 %), Staphylococcus simulans (2.08 %) and Staphylococcus saprophyticus (1.73 %) were isolated very rarely from the taken individual milk ewe samples. Sporadically, only in few cases, the others coagulase negative staphylococci were isolated (< 1 %): Staphylococcus cohnii cohnii, Staphylococcus sciuri, Staphylococcus closii, Staphylococcus lugdunensis, Staphylococcus auricularis and Staphylococcus equorum.
Strains of S. aureus were isolated from individual milk samples of 500 lacting cows from different areas in Slovakia (PH 1 - Košice region, 300 samples; PH 2 - Žilina region, 200 samples). The statistical significance between both dairy farms included in the experiment in the presence of genus Staphylococcus isolated on Baird-Parker agar from milk samples was p < 0,0001(***) . Totally, 122 milk samples were positive for the presence of Staphylococcus aureus (83 Košice regions, 52 Žilina regions). All 122 isolates of S. aureus were sensitive for the methicilin as detected by interpretative criteria developed by NCCLS (2002). For 122 S. aureus isolates, we compared antibiotic susceptibility results determined by the standardized agar diffusion assay with the PCR assay for the detection of antibiotic resistance mecA gene. For all isolates, we found a correlation between the results of the PCR and those of classical resistance testing. The obtained results were confirmed by PCR analysis, according to which, any of our tested isolate of S. aureus from all 122 individual milk samples from both experimental dairy farms were not positive for the presence of mecA gene coding the methicilin resistance. doi:10.5219/21
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