Cyclosporin A inhibits CD11a/CD18 adhesion molecules due to inhibition of TNF-α and IL-1β levels in the mouse model of pleurisy induced by carrageenan

Abstract

The mouse model of pleurisy induced by carrageenan is characterized by a significant enhancement of cell migration due to neutrophils 4 h after pleurisy induction. Forty-eight hours after pleurisy induction, a significant increase in cell migration due to mononuclear cells occurs. Recently, studies in our laboratory have demonstrated that cyclosporine A (CsA) inhibits leukocyte migration in the pleural cavity and lungs in the mouse model of pleurisy induced by carrageenan. In the present work we evaluated whether CsA was able to downregulate CD11a/CD18 adhesion molecule in the lungs, as well as TNFα and IL-1β levels in the fluid leakage of the pleural cavity in this model. Our results showed that CsA significantly decreased CD11a/CD18 in the lungs, as well as TNFα and IL-1β levels in the fluid leakage of the pleural cavity 4 h and 48 h after pleurisy induction. It is our hypothesis that the inhibitory effect elicited by CsA upon these adhesion molecules may be also be attributed to the downregulation of TNFα and IL-1β cytokines.

Calcineurin regulates innate antifungal immunity in neutrophils

“…Calcineurin then removes the inhibitory phosphates, allowing NFATs to translocate to the nucleus where they collaborate with other transcription factors, such as AP-1, to effect changes in gene transcription ( Clipstone and Crabtree, 1992 ). Although NFATs have been extensively studied in the context of T cells, relatively few studies have examined their function in myeloid lineages ( Losa García et al, 1996 ; Dalmarco et al, 2008 ; Zanoni et al, 2009 ).…”

The possible protective and therapeutic roles of fucoidan in cyclosporine-induced histological changes in the bone marrow and spleen in rats

“…CsA differs from other immunosuppressive in its weak myelotoxicity. Moreover, CsA inhibits the production of proinflammatory cytokines [7]. Moreover, CsA inhibits the production of proinflammatory cytokines [7].…”

Diethylcarbamazine Attenuates the Development of Carrageenan-Induced Lung Injury in Mice

“…The inflammatory process is invariably characterized by the production of prostaglandin, leukotrienes, histamine, bradykinin, platelet-activating factor, interleukins (IL), and migrating cells [ 10 ]. The recruitment of polymorphonuclear cells (PMNs) from the circulation to the inflamed tissue has a key function in the breakdown and remodeling of injured tissue [ 11 , 12 ]. Moreover, macrophages participate in the progression of experimental pleurisy by producing proinflammatory cytokines, such as tumor necrosis factor- α (TNF α ) and IL-1 β [ 13 ].…”