The possible protective and therapeutic roles of fucoidan in cyclosporine-induced histological changes in the bone marrow and spleen in rats

Eldien, Heba M. Saad; Omar, Hossam El-Din M.; Badary, Mohammed S; Al-Khatib, Bushra Y. H.

doi:10.1097/01.ehx.0000418510.89183.5f

Cited by 4 publications

(6 citation statements)

References 60 publications

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“…Most acini of parotid gland contained huge dilated rough endoplasmic reticulum with retained secretion [27]. Further insight into the rough endoplasmic reticulum dilatation in plasma cells was provided by other researchers who suggested that this might reveal an increase in their immunological activity [32]. Importantly, the acinar cell impairment has been investigated in several experimental models.…”

Section: Discussionmentioning

confidence: 99%

Effect of Fluoxetine on the Pancreas of Adult Male Albino Rats and the Possible Protective Role of Omega-3: Light and Electron Microscopic Study

Youssef¹

2017

IJCDA

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Depression is linked with a high risk of type 2diabetes (T2D). The affiliation between depression and diabetes might be correlated to depression itself and, or medications recommended. Significantly, the usage of selective serotonin reuptake inhibitors (SSRIs), the most commonly antidepressants increased the hazard of developing T2D. Nevertheless, the mechanism underlying this suggestion remains vague. Omega-3 had antioxidant and anti-inflammatory activity. So, there is developing evidence that consumption of omega-3 could be expedient. The present study was carried out to investigate the potential effect of omega-3 in the fluoxetine induced alterations in the pancreas of adult male albino rats. Forty adult male albino rats were divided into four groups. Group I served as a control group. Group II received a single daily dose of 300 mg/kg of omega-3. Group III received 24 mg/kg bw/day of fluoxetine hydrochloride. Group IV received omega-3 and fluoxetine as group II and III for 30 days. Light and electron microscopic investigations were carried out. Histological examination using H & E and Masson's Trichrome stain were carried out. The insulin expression in β cells was evaluated using immunohistochemistry. Morphometric results were subjected to statistical analysis. Investigation of group III (Fluoxetine group) showed distorted exocrine pancreas with thick interlobular septa that contained dilated congested blood vessels, cellular infiltration, and fat cells. Marked shrunken of the pancreatic islets was observed. Masson's trichrome stain showed increased collagen fibers deposition. Electron microscopic examination revealed that most of the acinar cells had irregular shaped nuclei with peripheral heterochromatin and wide capillaries. The cytoplasm of β cells had a variety of secretory granules. Most of them had an electron dense core with increased electron lucent halo however, few β granules were empty and coalesced. Omega-3 supplementation improved the morphology of Langerhans compared to fluoxetine group. Importantly, some pancreatic duct cells revealed a positive reaction against anti-insulin antibodies. The current results demonstrated that fluoxetine harmfully affected the histological structure of the pancreas. Omega-3 diminished effectively some histological, immunohistochemical and electron microscopic changes in a fluoxetine induced pancreatic injury. Omega-3 could stimulates β-cell regeneration from potent islet progenitor cells present in the ductal cells and these might lead to repair of the functional accomplishments of the injured pancreas to a great extent.

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Section: Discussionmentioning

confidence: 99%

Effect of Fluoxetine on the Pancreas of Adult Male Albino Rats and the Possible Protective Role of Omega-3: Light and Electron Microscopic Study

Youssef¹

2017

IJCDA

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“…showed that 4 weeks treatment with CSA causes significant decrease in Hb concentration mechanism of which is related to inhibition of erythropoietin hormone by CSA; this effect was also observed on culture medium of human Hep3 B cell line with addition of CSA 17 . Eldien et al had also seen bone marrow suppression in form of degenerating cells among hematopoietic cells after CSA treatment in rats 18 . The decrease could be due to decreased hematopoiesis, increased destruction of RBCs or slower rate of production as seen with CCl4 induced haematotoxicity 19 .…”

Section: Final Body Weights Of Animals In Controlmentioning

confidence: 95%

“…CSA also shows toxic effects in different tissues of the body. In bones it increases remodeling with appreciable bone loss while in bone marrow it inhibits the erythroid and stromal progenitors 6 . Cyclosporine has shown to increase the number of plasma cells and decrease the macrophages in humans 6 .…”

Section: Annals Abbasi Shaheed Hospital and Karachi Medical And Denta...mentioning

confidence: 99%

“…In bones it increases remodeling with appreciable bone loss while in bone marrow it inhibits the erythroid and stromal progenitors 6 . Cyclosporine has shown to increase the number of plasma cells and decrease the macrophages in humans 6 . In thymus of treated animals marked depletion of medullary region is noted 5 .…”

Section: Annals Abbasi Shaheed Hospital and Karachi Medical And Denta...mentioning

confidence: 99%

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Changes in Hematological Parameters of Sprague Dawley Rats with Use of Cyclosporine and Nigella Sativa

Yonus

Ali

Sajjad

et al. 2021

ashkmdc

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Objective: The objective of this study was to observe the signs of Cyclosporine toxicity and to analyze the ameliorative effects of Nigella Sativa (NS or Kalonji) on body weight and haematological parameters.Methods: This study was conducted in the Department of Anatomy, Baqai Medical University of Karachi during the month of February 2017. Thirty healthy male Sprague Dawley rats were selected from the Animal House of the university. After acclimatization, the animals were randomly divided into three groups, with 10 rats in each Group. Group A was the control group and received no intervention. Group B received treatment of oral Cyclosporine (15 mg/kg/day) in a single daily dose and Group C was given Cyclosporine (15 mg/kg/day) with Nigella Sativa seeds (450 mg/kg) as a protective agent. The bodyweight of rats in each group was measured at the start of the study period. The treatment was given for the duration of 21 days, after which the body weight was measured again. The rats were anaesthetized and blood was collected through cardiac puncture. Blood analysis for haemoglobin percentage, total leucocyte count and differential leucocyte count was done using haematology analyzer. Manual differential leucocyte was also done after staining the blood smear with Leishman's stain.Results: Final body weight of rats in Cyclosporine treated Group B was decreased significantly p<0.001 in comparison to control Group A and increased significantly p=0.001 in Nigella Sativa and Cyclosporine protected Group C. Blood parameters showed decreased mean haemoglobin p=0.03, decreased mean total leucocyte count p=0.003, increased mean neutrophil count p=0.27 and decreased mean lymphocyte count p=0.27 in Cyclosporine treated Group B while the mean values of these haematological parameters improved in Nigella Sativa protected Group C.Conclusion: The present study demonstrated that Cyclosporine caused a decrease in animal weight and toxic changes in haematological parameters while Nigella Sativa was helpful in ameliorating them

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“…FU has been extensively studied due to its varied biological properties in which it acts as an anticoagulant, antithrombotic, anti-inflammatory, antitumor, immunomodulatory, anti-complementary and antivirus agents, as well as it acts to reduce the blood lipids (Bilan et al, 2002;Li et al, 2008). Further, FU ameliorated the changes of bone marrow cells induced by CsA treatment (Eldien et al, 2012) and expressed activities against hepatopathy, uropathy, and kidney harms as well as it has a gastric protective effect (Kwak, 2014;Li et al, 2008). In addition, FU can inhibit the synthesis and release of ROS as well as it promotes its clearance that proved its antioxidative activity (Balboa et al, 2013;Josephine et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Ameliorated and antioxidant effects of Fucoidan against cyclosporine A-induced kidney injury in rats

Al-Khatib

Al-Hamdani

Gumaih

2019

JoBAZ

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Background: Cyclosporine A (CsA) is an immunosuppression agent used frequently in fields of organs transplantation and autoimmune diseases. However, CsA-induced kidney injury is a major clinical problem associated with CsA therapy in which the conceivable accountable mechanism is oxidative stress (OS). The present study evaluated the protective and ameliorated effects of fucoidan (FU) as an antioxidant and an anti-inflammatory agent against CsA-induced kidney injury. Methods: Male rats were randomly divided into three groups. The first group was served as a control group that administered olive oil orally and normal saline subcutaneously, the second group (CA) was treated with CsA orally, and the third group (CA + FU) was treated with CsA orally in concomitant with FU subcutaneously. Experimental animals were sacrificed 20 days following the treatment period and serum samples were analyzed for creatinine test. The homogenate of renal tissues was prepared for OS assessment. Light and ultrastructure microscopic kidney sections were prepared for histopathological examination. Results: Treatment of rats with CsA alone produced a significant increase in the levels of creatinine, nitric oxide (NO), and malondialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), catalase (CAT), and glucose 6 phosphate dehydrogenase (G6PD), whereas the level of glutathione reduced (GSH) was decreased. Some histopathological changes of the kidney tissue including tubular epithelial hypertrophy, vacuolizations, necrotic glomerulus cell, capillaries network shrinking, vascular congestion, interstitial infiltration, loss of apical microvilli, and deteriorated mitochondria were observed in CA group. Concomitant FU administration with CsA enhanced renal function, as indicated by the low level of creatinine. Moreover, FU ameliorated the oxidative status in kidney tissue as well as it provided histological protection against CsA-induced kidney injury. Conclusion: FU can develop a protective mechanism against kidney injury induced by CsA that mediated by OS.

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The possible protective and therapeutic roles of fucoidan in cyclosporine-induced histological changes in the bone marrow and spleen in rats

Cited by 4 publications

References 60 publications

Effect of Fluoxetine on the Pancreas of Adult Male Albino Rats and the Possible Protective Role of Omega-3: Light and Electron Microscopic Study

Effect of Fluoxetine on the Pancreas of Adult Male Albino Rats and the Possible Protective Role of Omega-3: Light and Electron Microscopic Study

Changes in Hematological Parameters of Sprague Dawley Rats with Use of Cyclosporine and Nigella Sativa

Ameliorated and antioxidant effects of Fucoidan against cyclosporine A-induced kidney injury in rats

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