Diethylcarbamazine Attenuates the Development of Carrageenan-Induced Lung Injury in Mice

Abstract

Diethylcarbamazine (DEC) is an antifilarial drug with potent anti-inflammatory properties as a result of its interference with the metabolism of arachidonic acid. The aim of the present study was to evaluate the anti-inflammatory activity of DEC in a mouse model of acute inflammation (carrageenan-induced pleurisy). The injection of carrageenan into the pleural cavity induced the accumulation of fluid containing a large number of polymorphonuclear cells (PMNs) as well as infiltration of PMNs in lung tissues and increased production of nitrite and tumor necrosis factor-α and increased expression of interleukin-1β, cyclooxygenase (COX-2), and inducible nitric oxide synthase. Carrageenan also induced the expression of nuclear factor-κB. The oral administration of DEC (50 mg/Kg) three days prior to the carrageenan challenge led to a significant reduction in all inflammation markers. The present findings demonstrate that DEC is a potential drug for the treatment of acute lung inflammation.

Diethylcarbamazine inhibits NF-κB activation in acute lung injury induced by carrageenan in mice

“…The effectiveness of DEC in the treatment of Tropical Pulmonary Eosinophilia (TPE) in clinical studies confirms this hypothesis and may be a potential indication for the use of this drug in other lung diseases such as COPD and ALI [3]. Recently, a number of studies have reported a marked anti-inflammatory property for DEC using asthma and acute lung injury models [4,5]. However, how DEC exerts this effect is still unknown, and no existing studies have investigated the effect of DEC in relation to key transcription factors that orchestrate the inflammatory response.…”

Protective effect of diethylcarbamazine inhibits NF-κB activation in isoproterenol-induced acute myocardial infarction rat model through the PARP pathway

“…In the present study, diethylcarbamazine significantly reduced the AMI-induced COX-2 protein expression in rats. Ribeiro et al (31) demonstrated that diethylcarbamazine attenuates carrageenan-induced lung injury in rats via COX-2 and iNOS expression. .…”

Diethylcarbamazine: Possible therapeutic alternative in the treatment of alcoholic liver disease in C57BL/6 mice

“…Five sections (5 μ m in thickness) of each group were cut and adhered to slides treated with 3‐amino‐propyl‐trietoxi‐silane (Sigma). Then, the immunohistochemical assays were performed according to the methodology of Ribeiro et al . The antibodies used were anti‐IL‐1 β antibody (1 : 11 dilution; ab9722; Abcam, Cambridge, UK), anti‐IL‐17 (1 : 50 dilution; sc‐7927; SantaCruz Biotechnology, Santa Cruz, CA, USA), anti‐IL‐10 (1 : 50 dilution; ab33471; Abcam), anti‐iNOS (1 : 250 dilution; ab3523; Abcam), anti‐COX‐2 (1 : 400; ab15191; Abcam), and TGF‐ β (1 : 100 dilution; ab66043; Abcam).…”

“…Cyclooxygenases are enzymes involved in the conversion of arachidonic acid to prostaglandin H2, the precursor of various compounds that are important inflammatory mediators . Recent studies in our laboratory have exploited this anti‐inflammatory action of DEC …”