The aim of the present study was to analyze the action of metformin on short-term memory, glial cell activation and neuroinflammation caused by experimental diabetic encephalopathy in C57BL/6 mice. Diabetes was induced by the intraperitoneal injection of a dose of 90mg/kg of streptozotocin on two successive days. Mice with blood glucose levels ≥200dl/ml were considered diabetic and were given metformin hydrochloride at doses of 100mg/kg and 200mg/kg (by gavage, twice daily) for 21 days. On the final day of treatment, the mice underwent a T-maze test. On the 22nd day of treatment all the animals were anesthetized and euthanized. Diabetic animals treated with metformin had a higher spatial memory score. The hippocampus of the diabetic animals presented reactive gliosis, neuronal loss, NF-kB signaling activation, and high levels of IL-1 and VEGF. In addition, the T-maze test scores of these animals were low. Treatment with metformin reduced the expression of GFAP, Iba-1 (astrocyte and microglial markers) and the inflammation markers (p-IKB, IL-1 and VEGF), while enhancing p-AMPK and eNOS levels and increasing neuronal survival (Fox-1 and NeuN). Treatment with metformin also improved the spatial memory scores of diabetic animals. In conclusion, the present study showed that metformin can significantly reduce neuroinflammation and can decrease the loss of neurons in the hippocampus of diabetic animals, which can subsequently promote improvements in spatial memory.
Mansonella ozzardi infections are common in the riverside
communities along the Solimões, Negro and Purus Rivers in the state of Amazonas (AM).
However, little is known about the presence of this parasite in communities located
in regions bordering AM and the state of Acre. The prevalence rate of M.
ozzardi infections was determined in blood samples from volunteers
according to the Knott method. A total of 355 volunteers from six riverine
communities were enrolled in the study and 65 (18.3%) were found to be infected with
M. ozzardi. As expected, most of the infections (25%) occurred in
individuals involved in agriculture, cattle rearing and fishing and an age/sex group
analysis revealed that the prevalence increased beginning in the 40-50-years-of-age
group and reached 33% in both sexes in individuals over 50 years of age. Based on the
described symptomatology, articular pain and headache were found to be significantly
higher among infected individuals (56 and 65% prevalence, respectively, p < 0.05).
Sera from volunteers were subjected to ELISA using a cocktail of recombinant proteins
from Onchocerca volvulus to evaluate the specificity of the test in
an endemic M. ozzardi region. No cross-reactions between M.
ozzardi-infected individuals and recombinant O. volvulus
proteins were detected, thus providing information on the secure use of this
particular cocktail in areas where these parasites are sympatric.
This study investigated the anti-inflammatory effects of DEC on the CCl4-induced hepatotoxicity in C57BL/6 mice. Chronic inflammation was induced by i.p. administration of CCl4 0.5 μL/g of body weight through two injections a week for 6 weeks. DEC (50 mg/kg) was administered by gavage for 12 days before finishing the CCl4 induction. Histological analyses of the DEC-treated group exhibited reduced inflammatory process and prevented liver necrosis and fibrosis. Immunohistochemical and immunofluorescence analyses of the DEC-treated group showed reduced COX-2, IL1β, MDA, TGF-β, and αSMA immunopositivity, besides exhibiting decreased IL1β, COX-2, NFκB, IFNγ, and TGFβ expressions in the western blot analysis. The DEC group enhanced significantly the IL-10 expression. The reduction of hepatic injury in the DEC-treated group was confirmed by the COX-2 and iNOS mRNA expression levels. Based on the results of the present study, DEC can be used as a potential anti-inflammatory drug for chronic hepatic inflammation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.