CXCR4-SDF-1 interaction potentially mediates trafficking of circulating tumor cells in primary breast cancer

Mego, Michal; Cholujová, Dana; Minárik, Gabriel; Sedláčková, Tatiana; Gronesova, Paulina; Karaba, Marián; Benca, Juraj; Cingelová, Silvia; Čierna, Zuzana; Manasova, Denisa; Pinďák, Daniel; Šufliarský, Jozef; Cristofanilli, Massimo; Reuben, James M.; Mardiak, Jozef

doi:10.1186/s12885-016-2143-2

Cited by 63 publications

(70 citation statements)

References 42 publications

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“…The CXCR4-SDF-1 interaction potentially mediates the trafficking of circulating tumor cells in primary breast cancer (36).…”

Section: Discussionmentioning

confidence: 99%

CoCl2 increases the expression of hypoxic markers HIF‑1α, VEGF and CXCR4 in breast cancer MCF‑7 cells

Zhang

2017

Oncol Lett

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Abstract. The aim of the present study was to investigate the effect of a hypoxic environment on the biological behavior of breast cancer MCF-7 cells, using CoCl 2 to mimic the hypoxia model in breast cancer cells. Using 50, 100, 150 and 200 µM CoCl 2 as a hypoxic inducer, a hypoxic model was established in MCF-7 cells in vitro. MTT, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) and western blotting assays were performed to detect MCF-7 cell proliferation under hypoxic conditions and the expression of the hypoxic markers hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and C-X-C motif chemokine receptor 4 (CXCR4) mRNA and that of the associated proteins. The RT-qPCR results revealed that there were no obvious changes in the expression of HIF-1α mRNA; however, the expression of CXCR4 and VEGF mRNA increased significantly following treatment with different CoCl 2 concentrations (P<0.05). The results of western blotting identified that CoCl 2 significantly induced the expression of HIF-1α, CXCR4 and VEGF proteins (P<0.05). The MTT assay revealed that different concentrations of CoCl 2 inhibited the proliferation of MCF-7 cells. The TUNEL assay demonstrated that CoCl 2 was able to trigger apoptosis of MCF-7 cells. Therefore, the results of the present study identified that CoCl 2 is able to control MCF-7 cell proliferation and apoptosis, also increasing the expression of HIF-1α, CXCR4 and VEGF. The present study may aid the discovery of a novel method to prevent cell damage and decrease cell proliferation in order to prevent the occurrence and development of breast cancer.

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“…The CXCR4-SDF-1 interaction potentially mediates the trafficking of circulating tumor cells in primary breast cancer (36).…”

Section: Discussionmentioning

confidence: 99%

CoCl2 increases the expression of hypoxic markers HIF‑1α, VEGF and CXCR4 in breast cancer MCF‑7 cells

Zhang

2017

Oncol Lett

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“…Mego et.al states SDF-1 related with circulating tumour cells and act as chemotactic signal. 18 It also found that SDF-1 is directly correlated with prognosis and tumour progression.…”

Section: Discussionmentioning

confidence: 91%

Association of SDF-1 with Metastasis in Breast Cancer Patient at Sanglah Hospital, Bali

et al. 2016

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Background: More than 24% breast cancer patients came to Sanglah Teaching Hospital with distant metastasis which cause 90% of cancer related death. Distant metastasis is complex process of interaction between tumor cells and its micro environment involving chemoattractant cytokines which lead circulating tumor cells toward target organs. One of the most common cytokines involved in metastasis of multiple tumor is SDF-1, produces by target organ or tumor cells it selves. However, only few studies ever evaluate the relationship between its concentrations in tumor tissue with metastasis. Method: A cross sectional analysis study was conducted involving clinical data and paraffin blocks from 46 patients. Samples were grouped into metastasis and non-metastasis group and level of tumor tissue SDF-1 was evaluated by immunohistochemistry method.

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“…E-F). Additionally, we compared our data for CXCR4, which is known to be expressed in peripheral blood CTCs from breast cancer patients (40). We found that both LCTCs and BCTCs expressed CXCR4 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Lymph-Circulating Tumor Cells show distinct properties to Blood-Circulating Tumor Cells and constitute extraordinary efficient metastatic precursors

Mohammed

et al. 2018

Preprint

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The molecular properties of tumor cells as they exit the primary tumor into the afferent lymphatics en route to the sentinel lymph nodes (SLNs) are not yet known. We developed an innovative technique that enables the collection of lymph and lymph-circulating tumor cells (LCTCs) en route to the SLN in immunocompetent animal model of breast cancer metastasis. We found that LCTCs and blood circulating tumor cells (BCTCs) as exited the primary tumor shared similar gene and protein expression profiles that were distinct from those of primary tumors and lymph node metastases (LNMs) despite their common parental cell origin. LCTCs but not BCTC exist in clusters, display a hybrid epithelial/mesenchymal phenotype and cancer stem cell-like properties and constitute extraordinarily efficient metastatic precursors. These results demonstrate that tumor cell metastasizing through the lymphatic are different from those spread by the blood circulation. The contribution of these cells to overall peripheral blood CTC is important in cancer therapy. Whether these two types of cells occur in cancer patients remain to be determined.

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CXCR4-SDF-1 interaction potentially mediates trafficking of circulating tumor cells in primary breast cancer

Cited by 63 publications

References 42 publications

CoCl2 increases the expression of hypoxic markers HIF‑1α, VEGF and CXCR4 in breast cancer MCF‑7 cells

CoCl2 increases the expression of hypoxic markers HIF‑1α, VEGF and CXCR4 in breast cancer MCF‑7 cells

Association of SDF-1 with Metastasis in Breast Cancer Patient at Sanglah Hospital, Bali

Lymph-Circulating Tumor Cells show distinct properties to Blood-Circulating Tumor Cells and constitute extraordinary efficient metastatic precursors

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