Custom synthesis of molecular imprinted polymers for biotechnological application

Abstract

A molecularly imprinted polymer (MIP) selective for tylosin was designed and synthesised using a computational method (MIP "dialling"). In re-binding experiments the MIP demonstrated high affinity for tylosin in aqueous solutions and in organic solvents. The synthesised polymer was tested for re-binding with the template and related metabolites such as tylactone, narbomycin and picromycin. The HPLC analysis showed that the computationally designed polymer is specific and capable of separating the template from its structural analogues. The MIP was capable of recovering tylosin from *Corresponding author.

Computational design of molecularly imprinted polymer for direct detection of melamine in milk

“…Determination of the stoichiometric ratio of template-FM was done via an established simulated annealing process, described elsewhere (16)(17)(18) using the SYBYL molecular modelling suite. A virtual cubic box containing one template molecule is packed with monomers until saturation capacity.…”

A Protocol for the Computational Design of High Affi nity Molecularly Imprinted Polymer Synthetic Receptors

“…The examples in table 2 range from (i) good imprinting factors [33,35]; (ii) high recovery of template from using solid phase extraction (SPE) [42,43,50,51]; (iii) controlled release [46,47]; (iv) dissociation constant in nM [35]; and (v) industrial applications [44,48]. This procedure therefore can be observed to produce excellent results with minimal time requirements, making this whole process highly effi cient in comparison with alternative approaches.…”

“…Tylosin [48] The polymer capacity for tylosin was estimated as 6.4 mg/g for MIP, which was suitable for practical applications…”

The requisite level of theory for the computational design of molecularly imprinted silica xerogels

“…Computational screening based on MM calculations taking into account ∼20 monomers against a molecular model of a template has been used as a standard protocol for MIP design (e.g. Subrahmanyam et al, 2001;Chianella et al, 2002;Piletsky et al, 2004) but, in principle, more reliable results can be obtained using Quantum mechanics.…”