Current possibilities of cervical precancerous lesions screening in Slovakia: prevalence of high risk human papillomavirus in patients with cytological diagnoses of atypical squamous cells of unknown significance

Abstract

Abstract:Introduction: It has been confi rmed, that there is a causal relationship between persistent infection of high risk human papillomavirus (HR-HPV) and the development of cervical cancer. In population of women older than 30 years HPV infection becomes a signifi cant etiological factor of precancerous lesion of the cervix, but HPV infection may spontaneously regress in the majority of the cases. Patients and methods:The analysed study group consisted of 397 samples with cytological diagnosis of atypical squamous cells of unknown signifi cance (ASCUS). All cases underwent HPV DNA testing using the Hybrid Capture 2 (HC2) assay. We analysed prevalence of HR-HPV and a viral load expressed as relative light units/ cut off ratio (RLU/CO) in different age groups with cytological diagnoses of ASCUS. Results: The prevalence of HR-HPV with cytological diagnoses of ASCUS was detected in 44 %. The prevalence of HR-HPV between patients aged 17-29 and between patients aged 30-40 was 55 % and 48 % respectively and we detected signifi cant reduction of prevalence (28 %) in patients older than 41 years. Conclusion: Based on the results of presented study we assumed that age the 40 and over is crucial for the development of serious precancerous lesions in Slovakia, thus this age group is the most suitable for HPV triage of ASCUS. As a refi nement of that type of ASCUS triage we recommend to add to the algorithm quantitative measurement of viral load in the specimens in the form of RLU/CO ratio (Fig. 3, Ref. 27). Text in PDF www.elis.sk.

Prevalencia de genotipos del papiloma virus humano en mujeres de la provincia del Azuay, Ecuador

“…(Monk & Wiley, 2003). Está comprobado que la infección por el VPH es una de las más frecuente infecciones de transmisión sexual y actualmente se considera a este virus como el agente causal del carcinoma cérvico-uterino (Kolnikova et al, 2014). Se ha detectado ADN de VPH en el 98% de carcinomas de cervix.…”