Current fluctuations of bilayer lipid membranes modified by glucose oxidase

Passechnik, V.I.; Hianik, Tibor; Ivanov, S. A.; Sivak, Branislav; Šnejdárková, Maja; Rehák, M.

doi:10.1016/s0302-4598(98)00104-4

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…Particularly, it is valid for the current fluctuations [20]. We have shown that when dc voltage was applied to the s-BLM, the current fluctuations of both positive and negative polarities have been observed.…”

Section: VI Passechnik Et Almentioning

confidence: 83%

“…The difficulties in understanding the structure of s-BLM are connected with different electrical properties of s-BLM and free standing, conventional BLM. Particularly, for s-BLM formed on the fresh cut surface of the tip of silver or platinum wire covered by a Teflon tube, it has been shown that the equivalent electrical scheme of s-BLM at low frequencies (tens of Hz) cannot be represented by a parallel connection of resistor and capacitor [6,7], which is traditional for conventional BLM. The capacitance of s-BLM at higher frequencies was larger than that of conventional s-BLM of the similar size.…”

Section: Introductionmentioning

confidence: 99%

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Specific Capacitance of Metal Supported Lipid Membranes

et al. 1998

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A model for the structure of supported bilayer lipid membranes (s-BLM) is assumed based on the measurement of specific capacitance, electrostriction and surface potential of the s-BLM formed on the tip of freshly cut Teflon or polymer coated stainless steel wire and of the electrolyte-metal interface, depending on the frequency of AC voltage. We have shown that s-BLM does not represent a continuous bilayer on a metal support. Its structure is like that of a continuous monolayer, three layers, or in certain case, also like that of a bilayer interrupted by 'islands' of metal support. The former ones are characterized by a smaller resistance in comparison with a lipid bilayer and with specific capacitance 20-50 times higher than that of conventional, free standing BLM. The assumed model allows us to understand the nature of a number of effects observed on s-BLM, particularly the behavior of current fluctuations. The nature of current fluctuations can be easily explained by fluctuations of the size of the 'islands' both towards increase and/or decrease of their diameter due to removal and/or appearance of new lipid molecules on a metal support.

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Section: VI Passechnik Et Almentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Specific Capacitance of Metal Supported Lipid Membranes

et al. 1998

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“…At higher voltages the current considerably increases and nonlinearly depends on voltage [6]. It has been shown [49] that up to voltages of 0.3± 0.4 V, sBLMs are characterized by speci®c capacitances similar to that of freely suspended membranes. However at higher voltages, the value of C s increases and approaches the values characteristic for a metal-electrolyte interface.…”

Section: Voltammetrymentioning

confidence: 99%

“…Current¯uctuations of sBLM have been studied by Passechnik et al [52]. It has been shown that in the frequency range of¯uctuations of 10±1000 Hz the dependence of spectral density, P, on the frequency of¯uc-tuations, f, can be described by 1af a , where a % 1.…”

Section: Current Fluctuationsmentioning

confidence: 99%

Biosensors Based on Thin Lipid Films and Liposomes

Nikolelis¹,

Hianik

Krull³

1999

Electroanalysis

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This article reports the theory and analytical applications of thin lipid ®lms. Recent advances of electrochemical devices based on lipid membranes have lead to reports of construction of biosensors for environmental and food applications, and may provide opportunities for commercial fabrication. The methods of formation of lipid membranes on various supports including metals (silver, gold, stainless steel), agar, conducting polymers and ultra®ltration membranes have provided stabilization of lipid ®lms with a diversity of analytical applications in real samples. Methods of immobilization and incorporation of various functional macromolecules are summarized. Several examples of the application of various methods for study of physical properties of supported bilayer lipid membranes are described. Applications of lipid-based biosensors in analytical chemistry for determination of compounds are demonstrated, including a diversity of chemical compounds such as environmental pollutants (ammonia and carbon dioxide, cyanide ions, etc.) and food toxins (a¯atoxin M 1 and direct detection of toxin in real samples such as milk and milk preparations). Methods for application of liposomes as a sensing system are also summarized.

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Studies on Electrocatalytical Kinetic Behavior of Horseradish Peroxidase and Assay for Hydrogen Peroxide at Salt Bridge Supported Bilayer Lipid Membrane

2001

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This article reports on the electrochemical behavior, relative to the Fe(III)‐Fe(II) conversion, of horseradish peroxidase (HRP) incorporated in a salt bridge‐supported bilayer lipid membrane (sb‐BLM) modified with lauric acid (LA). The incorporation of HRP was achieved by electrostatic interaction between HRP and LA anion sites in the sb‐BLM, which results in the enhancement of the electron exchange between the protein and the electrode surface. A quasireversible electron transfer was observed even in the absence of mediators. A novel and biological compatible biosensor was constructed. The electrocatalytically kinetic behavior of HRP was investigated with this biosensor. Furthermore, the cathodic peak current (IPc) of HRP was increased in the presence of hydrogen peroxide, the intensity of which is strictly dependent on the concentration of hydrogen peroxide. The relationship is ΔIPc(A)=[1.215 lnC H 2 O 2(mmol L−1)+1.018]×10−7. The effects of the coexisting substances and the pH values on the response of the biosensor were also investigated for optimum analytical performance.

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Current fluctuations of bilayer lipid membranes modified by glucose oxidase

Cited by 4 publications

References 6 publications

Specific Capacitance of Metal Supported Lipid Membranes

Specific Capacitance of Metal Supported Lipid Membranes

Biosensors Based on Thin Lipid Films and Liposomes

Studies on Electrocatalytical Kinetic Behavior of Horseradish Peroxidase and Assay for Hydrogen Peroxide at Salt Bridge Supported Bilayer Lipid Membrane

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