Biosensors Based on Thin Lipid Films and Liposomes

Nikolelis, Dimitrios P.; Hianik, Tibor; Krull, Ulrich J.

doi:10.1002/(sici)1521-4109(199901)11:1<7::aid-elan7>3.0.co;2-f

Cited by 94 publications

(48 citation statements)

References 66 publications

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“…[25,26] The phospholipids are used as biological membrane mimetic systems in studies involving the interaction between pharmacological drugs and cellular membranes. [27 -29] Recently, we have used the LbL technique [30] as a new approach to produce multilayered thin films containing biological phospholipids, which were applied as transducers in sensing units of an electronic tongue system to detect a phenothiazine pharmaceutical compound (methylene blue -MB).…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Ag nanoparticles into membrane mimetic systems composed by phospholipid layer‐by‐layer (LbL) films to achieve surface‐enhanced Raman scattering as a tool in drug interaction studies

Aoki

Aléssio

Saja

et al. 2009

J Raman Spectroscopy

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The synergistic effect produced by nanoparticles when incorporated into different systems used as analytical tools represents a growing research field nowadays. On the other hand, the study of interactions involving pharmacological drugs and biological membranes using phospholipids as mimetic systems is a research field already well established. Here, we combine both the anionic phospholipid dipalmitoyl phosphatidyl glycerol (DPPG) and negative Ag nanoparticles (AgNP) to form layer-by-layer (LbL) multilayered films using the cationic polymer poly(allylamine hydrochloride) (PAH) as the supporting polyelectrolyte, which were further investigated in the presence of a phenothiazine compound (methylene blue -MB). The molecular architecture of the LbL films in terms of controlled growth, morphology with micro and nanometer spatial resolutions, and dispersion of both AgNP and MB within the DPPG matrix was determined combining spectroscopy [ultraviolet-visible (UV-Vis) absorption and micro-Raman spectroscopy] and microscopy [scanning electron microscopy (SEM) and atomic force microscopy (AFM)].The results showed that the LbL films can be grown in a controlled way at nanometer thickness scale with the surface morphology susceptible to the presence of both AgNP and MB. The surface-enhanced phenomenon was applied to investigate the LbL films taking the advantage of the strong surface-enhanced resonance Raman scattering (SERRS) signal presented by the MB molecules. Besides, as MB is a pharmacological drug of interest, its molecular arrangements when dispersed in LbL films containing DPPG, which is the biological membrane mimetic system here, were investigated. In this case, the AgNP played a key role in achieving the MB SERRS signal.

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Section: Introductionmentioning

confidence: 99%

Incorporation of Ag nanoparticles into membrane mimetic systems composed by phospholipid layer‐by‐layer (LbL) films to achieve surface‐enhanced Raman scattering as a tool in drug interaction studies

Aoki

Aléssio

Saja

et al. 2009

J Raman Spectroscopy

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“…For this reason further research has focused on utilizing self-assembled molecular structures formed on a solid support as a tool for the attachment the enzyme molecules and to protect the solid support from possible interferences. Despite the fact that several techniques have been employed for this purpose, the biosensors based on thin lipid films and liposomes have been shown to be more useful [8]. This approach was well documented by several works utilizing lipid films for the development of glucose sensors [9,10,11,12].…”

Section: Introductionmentioning

confidence: 99%

Properties of glucose biosensors based on dendrimer layers. Effect of enzyme immobilization

2002

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The properties of glucose biosensors based on dendrimer layers on a gold support, which depend on the method of immobilization of glucose oxidase (GOX), were studied by amperometry. The kinetic parameters of enzymatic reactions, response time, sensitivity, detection limit, linear range, and enzyme turnover were determined. We showed that a more stable and sensitive sensor was obtained when GOX was immobilized on the dendrimer by crosslinking with glutaraldehyde in vacuum. This biosensor was stable for at least eight weeks. The response time was approximately 1.3 min, the detection limit of glucose was 25 micro M, and the apparent Michaelis-Menten constant was relative low ( K(m)=1.1+/-0.1 mM) in comparison with that for GOX in solution. The reason for these differences is discussed. The example of the application of the developed biosensors for the detection of mercury is also presented. The inhibitory effect of mercury on GOX activity was observed at mercury concentration of 100 nM.

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“…This method of formation of sBLMs was recently used in the development of biosensors for the detection of a wide range of compounds of biomedical, environmental, food, etc. interest (for example see [5]). These BLMs have been extensively characterized [15, 20 ± 22].…”

Section: Electrochemical Investigation Of Interactions Of Metal-suppomentioning

confidence: 99%

“…Lipid films have been widely used for the rapid detection or continuous monitoring of a wide range of compounds in foods and in the environment [5]. Such electrochemical detectors are simple to fabricate and can provide fast response and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Biosensors for the Rapid Detection of Dopamine Using Bilayer Lipid Membranes (BLMs) With Incorporated Calix[4]resorcinarene Receptor

et al. 2002

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This work explores the use of a calix[4]resorcinarene receptor (lipophilic macrocyclic host molecule) for the rapid electrochemical detection of dopamine using planar bilayer lipid membranes (BLMs). BLMs were composed of egg phosphatidylcholine (PC) and 35% (w/w) dipalmitoyl phosphatidic acid in which the receptor was incorporated. Freely‐suspended and metal supported BLMs modified with the resorcin[4]arene receptor were used as one shot sensors to rapidly detect this catecholamine. The interactions of this compound with freely‐suspended BLMs were found to be electrochemically transduced in the form of a transient current signal with duration of seconds, which reproducibly appeared within 8 s after exposure of the membranes to dopamine. The response time for these BLMs without incorporated receptor for dopamine was about 3 min. The magnitude of the transient current signal was related to the concentration of the stimulating agent in bulk solution in the micromolar range. The present electrochemical technique was also applied for the rapid and sensitive screening of dopamine using surface‐stabilized bilayer lipid membranes (sBLMs). The interactions of dopamine with sBLMs with incorporated receptor produced electrochemical ion current increases, which reproducibly appeared within a few seconds after exposure of the membranes to the stimulant. The use of the receptor in sBLMs increased the sensitivity of the method by 10‐fold. The current signal increases were related to the concentration of dopamine in bulk solution in the micromolar range. No interferences from ascorbic acid were noticed because of the use of the negatively charged lipids in membranes. The present technique can be used as one‐shot sensor for the rapid detection of these pharmaceutical substances and keep prospects for the selective determination of catecholamines in biofluids.

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Biosensors Based on Thin Lipid Films and Liposomes

Cited by 94 publications

References 66 publications

Incorporation of Ag nanoparticles into membrane mimetic systems composed by phospholipid layer‐by‐layer (LbL) films to achieve surface‐enhanced Raman scattering as a tool in drug interaction studies

Incorporation of Ag nanoparticles into membrane mimetic systems composed by phospholipid layer‐by‐layer (LbL) films to achieve surface‐enhanced Raman scattering as a tool in drug interaction studies

Properties of glucose biosensors based on dendrimer layers. Effect of enzyme immobilization

Biosensors for the Rapid Detection of Dopamine Using Bilayer Lipid Membranes (BLMs) With Incorporated Calix[4]resorcinarene Receptor

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