Curcumin-loaded nanoemulsion: a new safe and effective formulation to prevent tumor reincidence and metastasis

Guerrero, Simón; Inostroza-Riquelme, Mariela; Contreras-Orellana, Pamela; Díaz-García, Víctor; Lara, Pablo; Vivanco-Palma, Andrea; Cárdenas, Areli; Miranda, Víctor; Robert, Paz; Leyton, Lisette; Kogan, Marcelo J.; Quest, Andrew F. G.; Oyarzún-Ampuero, Felipe

doi:10.1039/c8nr06173d

Cited by 68 publications

(42 citation statements)

References 61 publications

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“…Previous studies proved that the release kinetics of curcumin was controlled by the diffusion following Higuchi model [5,6]. In vitro assays revealed that curcumin nanoemulsion was safe for normal human cells, but favorably cytotoxic to cancerous gastric, colon, breast and melanoma cells [7,8]. This indicates high selectivity of curcumin nanoemulsion against cancer cells, but low cytotoxicity to normal cells.…”

Section: Introductionmentioning

confidence: 88%

Curcumin-Loaded Nanoemulsion for Better Cellular Permeation

et al. 2020

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Curcumin nanoemulsion was prepared using coconut oil, Tween 80 (surfactant) and polyethylene glycol (co-solvent) with the addition of honey and glycerol as additives. The nanoemulsion was optimized and systematically characterized for transdermal delivery. Small particle size (15.92 nm), low polydispersity index (0.17) and slight acidic (pH 4.18) curcumin nanoemulsion was obtained without any chemical degradation based on the Fourier transform infrared (FTIR) spectrum. The incorporation of curcumin inside nanoglobul improved curcumin stability and skin permeability. Its high permeability can be seen from Nile dyed curcumin in different layers of skin through fluorescent imaging. The release kinetic of curcumin followed the Higuchi model, which explains why the skin permeation was a Fickian diffusion-controlled process because the Korsmeyer constant was proven to be 0.3 (<0.5). Nanoencapsulation slightly decreased the antioxidant capacity of curcumin for about 7.9% compared to its free counterpart. It showed low cytotoxicity (EC50 2.3552 µg/mL) to human skin fibroblasts. Cell death was noticed at a high concentration (2.5 µg/mL) of treatment. Curcumin was also found to promote wound closure at low concentration 0.1563 µg/mL and was comparable with the performance of ascorbic acid based on scratch assay. Therefore, this nutritious curcumin nanoemulsion is a promising transdermal delivery system for topical application.

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Section: Introductionmentioning

confidence: 88%

Curcumin-Loaded Nanoemulsion for Better Cellular Permeation

et al. 2020

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“…12,13 However, some shortcomings of CUR have limited its clinical application, such as low solubility, poor stability, and low absorption rates. 14 To solve these problems, hyaluronic acid (HA), a negatively charged water-soluble natural chain mucopolysaccharide, was used to modify CUR via covalent bonding. Prior research found that the HA-specic receptor CD44 can be expressed on the surface of various cancer cells, such as breast cancer, ovarian cancer, and lung adenocarcinoma cells.…”

Section: Introductionmentioning

confidence: 99%

CD44-targeted hyaluronic acid–curcumin reverses chemotherapeutics resistance by inhibiting P-gp and anti-apoptotic pathways

et al. 2019

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“…Turmeric or natural curcumoids have been reported to have antioxidant [48], anti-inflammatory [49], and antimicrobial activities [50]. CURC has recently shown anti-tumor properties, relying on the inhibition of oncogenic/pro-survival STAT-3 and NF-kB-dependent pathways, of the pro-invasive factor Sp-1, of tumor-associated inflammation [51,52]. Interestingly, these effects are accompanied by a low toxicity against non-transformed cells [53], suggesting a sort of selectivity against tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Curcumin-Loaded Solid Lipid Nanoparticles Bypass P-Glycoprotein Mediated Doxorubicin Resistance in Triple Negative Breast Cancer Cells

et al. 2020

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Multidrug resistance (MDR) is a critical hindrance to the success of cancer chemotherapy. The main thing responsible for MDR phenotypes are plasma-membranes associated with adenosine triphosphate (ATP) Binding Cassette (ABC) drug efflux transporters, such as the P-glycoprotein (Pgp) transporter that has the broadest spectrum of substrates. Curcumin (CURC) is a Pgp inhibitor, but it is poorly soluble and bioavailable. To overcome these limitations, we validated the efficacy and safety of CURC, loaded in biocompatible solid lipid nanoparticles (SLNs), with or without chitosan coating, with the goal of increasing the stability, homogeneous water dispersibility, and cellular uptake. Both CURC-loaded SLNs were 5–10-fold more effective than free CURC in increasing the intracellular retention and toxicity of doxorubicin in Pgp-expressing triple negative breast cancer (TNBC). The effect was due to the decrease of intracellular reactive oxygen species, consequent inhibition of the Akt/IKKα-β/NF-kB axis, and reduced transcriptional activation of the Pgp promoter by p65/p50 NF-kB. CURC-loaded SLNs also effectively rescued the sensitivity to doxorubicin against drug-resistant TNBC tumors, without signs of systemic toxicity. These results suggest that the combination therapy, based on CURC-loaded SLNs and doxorubicin, is an effective and safe approach to overcome the Pgp-mediated chemoresistance in TNBC.

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Curcumin-loaded nanoemulsion: a new safe and effective formulation to prevent tumor reincidence and metastasis

Abstract: We report on the development of a new curcumin-loaded nanoemulsion (CUR-NEM) that is highly effective in preventing post-surgery tumor reincidence and metastasis.

Cited by 68 publications

References 61 publications

Curcumin-Loaded Nanoemulsion for Better Cellular Permeation

Curcumin-Loaded Nanoemulsion for Better Cellular Permeation

CD44-targeted hyaluronic acid–curcumin reverses chemotherapeutics resistance by inhibiting P-gp and anti-apoptotic pathways

Curcumin-Loaded Solid Lipid Nanoparticles Bypass P-Glycoprotein Mediated Doxorubicin Resistance in Triple Negative Breast Cancer Cells

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