Curcumin-functionalized silk materials for enhancing adipogenic differentiation of bone marrow-derived human mesenchymal stem cells

Li, Chunmei; Luo, Tingting; Zheng, Zhaozhu; Murphy, Ana A.; Wang, Xiaoqin; Kaplan, David L.

doi:10.1016/j.actbio.2014.08.009

Cited by 46 publications

(34 citation statements)

References 34 publications

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“…37–40 Silk materials used in these studies were mainly air-dried films. Drying process usually takes a few hours to overnight depending on the film thickness and time/temperature used.…”

Section: Discussionmentioning

confidence: 99%

DNA preservation in silk

et al. 2017

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The structure of DNA is susceptible to alterations at high temperature, pH, irradiation and exposure to DNase. Options to protect and preserve DNA during storage are important for applications in genetic diagnosis, identity authentication, drug development and bioresearch. In the present study, the stability of total DNA purified from human dermal fibroblast cells as well as plasmid DNA was studied in silk protein materials. The DNA/silk mixtures were stabilized on filter paper (silk/DNA+filter) or filter paper pre-coated with silk and treated with methanol (silk/DNA+PT-filter) as a route to practical utility. After air-drying and water extraction, 50–70% of the DNA and silk could be retrieved and showed a single band on electrophoretic gels. The 6% silk/DNA+PT-filter samples provided improved stability in comparison to 3% silk/DNA+filter and DNA+filter for DNA preservation, with ~40% of the band intensity remaining at 37 °C after 40 days and ~10% after exposure to the UV light for 10 hours. Quantitative analysis using the PicoGreen assay confirmed the results. The use of Tris/Borate/EDTA (TBE) buffer enhanced the preservation and/or extraction of the DNA. The DNA extracted after storage maintained integrity and function based on serving as a functional template for PCR amplification of the gene for zinc finger protein 750 (ZNF750) and for transgene expression of red fluoresence protein (dsRed) in HEK293 cells. The high molecular weight and high content of crystalline beta-sheet structure formed on the coated surfaces likely accounted for the preservation effects observed for the silk/DNA+PT-filter samples. Although similar preservation effects were also obtained for lyophilized silk/DNA samples, the rapid and simple processing available with the silk-DNA-filter membrane system makes it appealing for future applications.

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“…37–40 Silk materials used in these studies were mainly air-dried films. Drying process usually takes a few hours to overnight depending on the film thickness and time/temperature used.…”

Section: Discussionmentioning

confidence: 99%

DNA preservation in silk

et al. 2017

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“…The release rate was enhanced by the addition of surfactant to increase the solubility of hydrophobic molecules. On previous work, Li et al (2015) also reported that the release of curcumin from silk hydrogel films was significantly enhanced when the release assay was performed in PBS with Tween80 and methanol supplement compared to that of PBS without supplement [38]. The pH of the solution was found to have had a minor effect on the dissolution of curcumin, but the addition of surfactants to the solution was observed to significantly increase the molecule's solubility.…”

Section: Release Assaymentioning

confidence: 99%

Characteristics of Curcumin-Loaded Bacterial Cellulose Films and Anticancer Properties against Malignant Melanoma Skin Cancer Cells

et al. 2018

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Curcumin-loaded bacterial cellulose films were developed in this study. Curcumin was absorbed into never-dried bacterial cellulose pellicles by 24-h immersion in solutions of curcumin in the range of 0.2-1.0 mg /mL. The curcumin-loaded bacterial cellulose pellicles were then air-dried and characterized. The mechanical properties of curcumin-loaded bacterial cellulose films, particularly the stretching properties, appeared to be lower than those of bacterial cellulose film. This was especially evident when the loading concentration of curcumin was higher than 0.4 mg/mL. Fourier-transform infrared spectroscopy analysis indicated an interaction between bacterial cellulose microfibrils and curcumin. Controlled release of curcumin was achieved in buffer solutions containing Tween 80 and methanol additives, at pH 5.5 and 7.4. Curcumin-loaded bacterial cellulose films prepared with curcumin solutions at concentrations of 0.5 and 1.0 mg/mL displayed antifungal activities against Aspergillus niger. They also exhibited anticancer activity against A375 malignant melanoma cells. No significant cytotoxic effect was observed against normal dermal cells, specifically, human keratinocytes and human dermal fibroblasts.

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“…In particular, it has been suggested that curcumin might concentrate at the material surface or in the cell membrane in contact with the material rather than being released into the medium. 45 …”

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confidence: 99%

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Mouthuy¹,

Škoc²,

Gašparović³

et al. 2017

IJN

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Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 μM, respectively), which are near or above the known toxicity threshold of curcumin (~10 μM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters.

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Curcumin-functionalized silk materials for enhancing adipogenic differentiation of bone marrow-derived human mesenchymal stem cells

Cited by 46 publications

References 34 publications

DNA preservation in silk

DNA preservation in silk

Characteristics of Curcumin-Loaded Bacterial Cellulose Films and Anticancer Properties against Malignant Melanoma Skin Cancer Cells

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

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