Curcumin-loaded bacterial cellulose films were developed in this study. Curcumin was absorbed into never-dried bacterial cellulose pellicles by 24-h immersion in solutions of curcumin in the range of 0.2-1.0 mg /mL. The curcumin-loaded bacterial cellulose pellicles were then air-dried and characterized. The mechanical properties of curcumin-loaded bacterial cellulose films, particularly the stretching properties, appeared to be lower than those of bacterial cellulose film. This was especially evident when the loading concentration of curcumin was higher than 0.4 mg/mL. Fourier-transform infrared spectroscopy analysis indicated an interaction between bacterial cellulose microfibrils and curcumin. Controlled release of curcumin was achieved in buffer solutions containing Tween 80 and methanol additives, at pH 5.5 and 7.4. Curcumin-loaded bacterial cellulose films prepared with curcumin solutions at concentrations of 0.5 and 1.0 mg/mL displayed antifungal activities against Aspergillus niger. They also exhibited anticancer activity against A375 malignant melanoma cells. No significant cytotoxic effect was observed against normal dermal cells, specifically, human keratinocytes and human dermal fibroblasts.