Curcumin Analogue A501 induces G2/M Arrest and Apoptosis in Non-small Cell Lung Cancer Cells

Abstract

Curcumin and its analogues have been reported to exert anti-cancer activity against a variety of tumors. Here, we reported A501, a new curcumin analogue. The effect of A501 on cell viability was detected by MTT assay, the result showed that A501 had a better inhibiting effect on the four non-small cell lung cancer (NSCLC) cells than that of curcumin. Moreover, Colony forming experiment showed A501 significant restrained cell proliferation. Flow cytometry displayed A501 can cause G2/M arrest and induce apoptosis. Western blotting showed that A501 decreased the expression of cyclinB1, cdc-2, bcl-2, while increased the expression of p53, cleaved caspase-3 and bax. In conclusion, curcumin analogues A501 played antitumor activity by inhibiting cell proliferation and inducing apoptosis of NSCLC cells. And it was likely to be a promising starting point for the development of curcumin-based anticancer drugs.

Ethyl 1-(4-chlorobenzyl)-3-phenyl-1H-pyrazole-5-carboxylate

“…For background to the title compound, see: Ge et al (2007Ge et al ( , 2009Ge et al ( , 2011. For a related compound, see: Xia et al (2007).…”

Mannich Curcuminoids as Potent Anticancer Agents

“…Although numerous preclinical and clinical studies have demonstrated the biological potential of curcumin to treat cancer patients, either alone or in combination with already existing therapeutic regimens , the clinical application of curcumin has been limited by its low potency and poor pharmacokinetics . Therefore, in order to improve its activity and pharmaceutical profile, several structurally related compounds have recently been synthesised and evaluated as anticancer agents .…”

The Interplay between Autophagy and Apoptosis Induced by One Synthetic Curcumin Derivative Hydrazinobenzoylcurcumin in A549 Lung Cancer Cells

“…Curcumin analogues or derivatives are important synthetic chemicals in the research of tumor or cancer treatment, and there are many reports on the studies of their antitumor activities or mechanisms [16,17,20]. In the past few years, we also have focused on the curcumin analogues and carried out some experiments on the screening of derivatives with antiproliferative effects and their biological mechanisms of inducing cell death [21,22].…”