The Interplay between Autophagy and Apoptosis Induced by One Synthetic Curcumin Derivative Hydrazinobenzoylcurcumin in A549 Lung Cancer Cells

Abstract

The two important cell autonomic responses, autophagy, and apoptosis, play critical roles in cellular homeostasis and survival. By studying of the synthetic curcumin derivative hydrazinobenzoylcurcumin (HBC), we revealed that it could induce autophagy in nonsmall lung cancer cells (A549). Here, we use the Hoechst 33342 staining, Annexin V/propyliodide double dyeing and Western blotting analysis of PARP protein to demonstrate that HBC could also induce apoptosis in A549 cells. Apoptosis inhibitor (Z-VAD-FMK, 10 μM) treatment helps to promote the cells survival. Moreover, inhibition of apoptosis-promoted HBC-induced autophagy of A549 cells by morphological detection and Western blotting analysis (vice versa). These data indicate that there exist some interconnections between the autophagy and apoptosis induced by HBC. The following work will be carried out to characterize the specific regulation processes between the two cell pathways in A549 cells.

Curcumin induced autophagy anticancer effects on human lung adenocarcinoma cell line A549

“…First, monitored the formation of autophagic vesicles (AVs) by AO staining. Autophagosomes fuse with lysosomes forming antophagolysosomes as acidic vesicular organelles (AVOs) can bind AO in the process of cells autophagy ( 15 ). AO can stain nuclear and cytoplasm with bright green and make AVOs with bright red.…”

A curcumin derivative hydrazinobenzoylcurcumin suppresses stem‐like features of glioblastoma cells by targeting Ca2+/calmodulin‐dependent protein kinase II

“…26,28,29 HBC directly binds to the C-terminal hydrophobic pocket of CaM in a Ca 2+ -dependent manner and thus downregulates Ca 2+ /CaM-dependent proliferative and proangiogenic signaling pathways. 26,28,29 HBC directly binds to the C-terminal hydrophobic pocket of CaM in a Ca 2+ -dependent manner and thus downregulates Ca 2+ /CaM-dependent proliferative and proangiogenic signaling pathways.…”

“…24 These problems can be overcome by the creation of synthetic derivatives. 28 Furthermore, HBC inhibited Ca 2+ /CaM-dependent activation of hypoxia-inducible factor-1, resulting in an effective decrease in tumor angiogenesis. 25 HBC induced G 0 /G 1 cell cycle arrest of colon cancer cells and suppressed the growth of castration-resistant prostate cancer and oral cancer in mice, through downregulation of CaM-regulated signaling pathways.…”

Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells

“…We therefore speculated that autophagy-related cell death may play a key role in the high concentration of curcumin, and further experiments need to be carried out to test it. The signaling pathways and molecules of apoptosis and autophagy are widely interconnected, and many efforts have also been made to illustrate the interplay between them [ 37 , 38 ]. When the level of stimulation is low, the process of apoptosis is instructed to occur and autophagy is also induced to prosurvive at the same time.…”