Culturing substrates influence the morphological, mechanical and biochemical features of lung adenocarcinoma cells cultured in 2D or 3D

Prina-Mello, Adriele; Jain, Namrata G.; Liu, Baiyun; Kilpatrick, Jason I.; Tutty, Melissa Anne; Bell, Alan P.; Jarvis, Suzanne P.; Volkov, Yuri; Movia, Dania

doi:10.1016/j.tice.2017.11.003

Cited by 25 publications

(17 citation statements)

References 113 publications

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“…12 To confirm these findings, we performed life-cell AFM measurements in a region of the cell where the KFs are suspended between the flat cell periphery and the elevated nuclear region to avoid a potential restraint of the AFM probe by the underlying substrate. The E-moduli in our measurements were well within the range of previous studies 54 anddespite considerable differences between cells with the same mimicked phosphorylation site-both mutants mimicking phosphorylation of K18-S34 (K18-YFP-S34E and K18-YFP-S34D) reduced the mean elastic moduli in the respective cells ( Figure 6B). Interestingly, Sivaramakrishnan et al 9 observed in their experiments an increase in cell stiffness after 1 hour of mechanical stress, however, this study used primary cells, assessed the storage modulus and shear stress instead of cell stretch was applied.…”

Section: Discussionsupporting

confidence: 88%

Acute effects of cell stretch on keratin filaments in A549 lung cells

et al. 2020

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The group of intermediate filaments (IFs) comprise numerous different subtypes with keratin filaments (KFs) being characteristic for epithelia. The functions of KFs vary from signaling to coping mechanisms for cell stress 1-3 and their mere abundance and big surface area 4 might facilitate even more, yet, unrecognized roles in the cell. However, KFs' classic role as stretch resistant mechanical stabilizers of the cell 2 remains keratin's most outstanding feature. KFs' tensile resistance is founded on a filament architecture with non-globular subunits providing ample space for overlap between subunits and enabling a rope-like suprastructure. Briefly, a

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Section: Discussionsupporting

confidence: 88%

Acute effects of cell stretch on keratin filaments in A549 lung cells

et al. 2020

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“…On the latter, the contact surface between the membrane and the surface is very large, while in a scaffold, the cell membrane interacts with the ECM-mimicking scaffold at discrete locations [94]. This difference results in a stretched morphology with a well-defined cytoskeleton for cells grown on 2D substrates, whereas, in 3D scaffolds, the cytoskeleton typically re-organizes [95][96][97]. Note that, despite its difference with 2D culture systems, the morphology of cells embedded in a 3D scaffold resembles more the in vivo physiology [98,99].…”

Section: Scaffold-embedded Cellsmentioning

confidence: 99%

From 2D to 3D Cancer Cell Models—The Enigmas of Drug Delivery Research

2020

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Over the past decades, research has made impressive breakthroughs towards drug delivery systems, resulting in a wide range of multifunctional engineered nanoparticles with biomedical applications such as cancer therapy. Despite these significant advances, well-designed nanoparticles rarely reach the clinical stage. Promising results obtained in standard 2D cell culture systems often turn into disappointing outcomes in in vivo models. Although the overall majority of in vitro nanoparticle research is still performed on 2D monolayer cultures, more and more researchers started acknowledging the importance of using 3D cell culture systems, as better models for mimicking the in vivo tumor physiology. In this review, we provide a comprehensive overview of the 3D cancer cell models currently available. We highlight their potential as a platform for drug delivery studies and pinpoint the challenges associated with their use. We discuss in which way each 3D model mimics the in vivo tumor physiology, how they can or have been used in nanomedicine research and to what extent the results obtained so far affect the progress of nanomedicine development. It is of note that the global scientific output associated with 3D models is limited, showing that the use of these systems in nanomedicine investigation is still highly challenging.

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“…[ 69 ] Recent research has indicated that, compared with a 2D environment where some loss of functionality in post‐differentiation happens as a result of induced abnormal phenotypae, a 3D environment mimicks native tissues and stimulate and sustain the functionality of cells. [ 70 ]…”

Section: D Printed Graphene Structures For Tissue Engineeringmentioning

confidence: 99%

Recent Progress in 3D Printing of 2D Material‐Based Macrostructures

Yang

Zhou

Yang

et al. 2020

Adv Materials Technologies

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2D materials have a range of unique properties and are promising building blocks for the fabrication of macroscopic materials for many applications ranging from flexible electronics to energy storage devices. The development of effective methods to fabricate 2D material‐based macroscopic materials with designed structures is the key to enabling high performance. Lately, 3D printing has emerged as a new technique to assemble such materials. Compared to conventional fabrication methods, 3D printing techniques have a high degree of customization of the structure with a broad range of domain sizes from nanometers to centimeters, and thereby give the resulting products additional structure‐related functionalities. Recent advances in the fabrication of 2D material‐based macrostructures using 3D printing techniques and their uses in different fields are reviewed. Challenges and opportunities for the future development of the topic are also discussed.

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Culturing substrates influence the morphological, mechanical and biochemical features of lung adenocarcinoma cells cultured in 2D or 3D

Cited by 25 publications

References 113 publications

Acute effects of cell stretch on keratin filaments in A549 lung cells

Acute effects of cell stretch on keratin filaments in A549 lung cells

From 2D to 3D Cancer Cell Models—The Enigmas of Drug Delivery Research

Recent Progress in 3D Printing of 2D Material‐Based Macrostructures

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