Culturing conditions affecting the production of anthocyanin in suspended cell cultures of strawberry

Quantitative determination of cultured strawberry-cell heterogeneity by image analysis: effects of medium modification on anthocyanin accumulation

“…Modified LS media were prepared by reference to previous papers [11,12]. The methods were as follows.…”

“…Anthocyanins are produced by various plant-cell cultures, including wild carrot [1,2], sweet potato [3], grape [4][5][6][7][8] and strawberry [9][10][11][12][13] cultures. It has been well documented that anthocyanin accumulation in plant cell cultures is strongly influenced by culture conditions, including light intensity [4,12,[14][15][16], plant growth regulators [10,11], medium components [4,12,17] and sugar concentration [2,[5][6][7][8]11,12,14]. However, most of these reports have relied on absorbance measurement after pigment extraction, and this extraction method can only provide the average level of pigment accumulation.…”

“…Moreover, even in highly pigmented plant-cell cultures, a considerable number of non-pigmented white cells are usually observed. Several studies have examined the percentages of pigmented cells among total cells by counting them under a microscope [4][5][6]12] and reported the wide distribution of anthocyanin content in cultured cells. In these cases, the pigmented cells were identified irrespective of the amount of the accumulated pigment and the identification depended on the criteria of individual examiners.…”

Enhanced anthocyanin production by repeated-batch culture of strawberry cells with medium shift

“…The major hindrances to commercialization are slow growth, low product concentration, and low productivity in plant cell cultures. To circumvent these problems, several methods have been proposed, e.g., genetic manipulation and clonal selection (Nozue et al 1987;Payne et al 1991), optimization of culture conditions (Drapeau et al 1986;Mori and Sakurai 1994;Pepin et al 1995;Sato et al 1996;Zhang et al 1997), and process system (Zhang et al 1999). The optimization of process system is easy to manipulate, and sometimes a significant improvement can be expected.…”

“…Strawberry cell suspension culture has become established for the production of anthocyanin and batch mode was adopted for all these cultures (Hong et al 1989;Mori et al 1993;Sato et al 1996;Zhang et al 1997Zhang et al , 1998Zhang et al , 1999. It seems a favorable and necessary undertaking to explore process optimization to enhance anthocyanin productivity in strawberry cell culture.…”