Cultured Human Hair Follicles and Growth Factors

Philpott, Michael P.; Sanders, Debbie; Kealey, Terence

doi:10.1038/jid.1995.61

Cited by 30 publications

(19 citation statements)

References 3 publications

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“…The inhibitory effects of IL-1 a on hair folli cle elongation in vitro ( fig. 3-5) are compara ble to those previously reported [6,7] and confirm that IL-l is a negative signal for the growth of human hair follicles, at least in vitro. In addition, we provide new evidence that the bulb of the hair follicle is degraded upon IL-la treatment and that the hair follicle is very sensitive to IL-la ( fig.…”

Section: Discussionsupporting

confidence: 75%

“…Skin Pharmacol 1996;9:366-375 369 Follicle suits are in agreement with those previously reported by others [6,7], In fact, the most dra matic effect of IL-la was to rapidly decrease the number of growing follicles. Figure 4 shows that the effects of IL-la are dosedependent.…”

Section: Inflammatory Cytokines In Hairsupporting

confidence: 81%

“…Individual human hair follicles from facelift sur gery (exclusively from female patients) were dissected according to previous reports from others [6,7,11], Briefly, 5 x 10-mm strips of scalp were cut at the dermo-epithelial junction. Anagen follicles with their peri follicular external conjunctive layer were then isolated using fine forceps.…”

Section: Human Hair Follicle Isolation and Culture In Vitromentioning

confidence: 99%

“…5]. Among the other factors that might control the hair cycle, interleukin (IL)-l has been reported as an inhibitor of hair folli cle growth in vitro [6,7] and in vivo in IL-la transgenic mice which specifically overex pressed IL-Iu in the skin [8]. IL-la and IL-1|3 are non-specific mediators of inflammation, active at low concentrations (10~15 M), simi lar to those of endogenous hormones [ref.…”

Section: Introductionmentioning

confidence: 99%

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Pro-Inflammatory Cytokine Cascade in Human Plucked Hair

Mahé

Buan

Billoni

et al. 1996

Skin Pharmacol Physiol

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Using reverse transcriptase polymerase chain reaction we showed that freshly plucked human anagen hair expressed both type 1 (80 kD) and type 2 (60 kD) interleukin (IL)-1 receptor mRNAs. The IL-1 receptor type 1 was functional since after in vitro stimulation of plucked hair with IL-1Α, we observed the induction of mRNA(s) for the inflammatory cytokines IL-1Β, tumour necrosis factor Α and IL-6 as well as for the chemokines monocyte chemotactic and activating factor and IL-8. In addition, the growth of dissected human anagen hairs in culture in vitro was significantly and dose-depen-dently inhibited by IL-1Α as a consequence of hair bulb degradation. These observations, together with those of other authors in IL-1Α transgenic mice evidence the inhibitory role of IL-1 on human hair growth. Therefore, in order to identify individuals with high inflammatory potential in their hair follicle environment, we designed a rapid and simple assay to detect variations in the level of IL-1Α production in the overnight supernatant of plucked hairs in culture. We observed that 32.7% of the specimens from the volunteers tested (n = 116) could be considered highly inflammatory in terms of IL-1Α production. Altogether, these results suggest that in alopecia androgenetica, hair growth might be negatively influenced by IL-1, directly produced by the outer root sheath keratinocytes. Consequently, identifying the ‘inflammatory alopecic individual’ might be of clinical interest to discriminate among individuals for whom anti-IL-1 strategies might be of therapeutic relevance.

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Section: Discussionsupporting

confidence: 75%

Section: Inflammatory Cytokines In Hairsupporting

confidence: 81%

Section: Human Hair Follicle Isolation and Culture In Vitromentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pro-Inflammatory Cytokine Cascade in Human Plucked Hair

Mahé

Buan

Billoni

et al. 1996

Skin Pharmacol Physiol

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“…This process that occurs at least in one-third of AGA cases [10,[26][27][28][29] initiates at infundibulum mediated by bacteria, UV irradiation, chemical and/or mechanical stress. Keratinocytes rapidly respond to stressors releasing interleukin-1α (IL-1α) [30,31] that was shown to inhibit hair growth in vitro [32][33][34]. A transcriptional cascade is activated, increasing pro-inflammatory cytokines (IL-1α, IL-1β, TNFα), chemokines (IL-8, MCPs), collagenases and others.…”

Section: Pathophysiology Of Agmentioning

confidence: 99%

Androgenic alopecia and dutasteride in hair mesotherapy: A short review

Busanello¹,

Turcatel²

2018

Our Dermatol Online

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Cytokines and growth factors influence hair growth in vitro. Possible implications for the pathogenesis and treatment of alopecia areata

et al. 1996

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Factors that influence the growth of the anagen hair follicle or initiate the switch to a catagen growth pattern have so far not been definitely determined, but there is increasing evidence that cytokines and growth factors play an important role during these processes. Recently we detected an aberrant in situ expression pattern of cytokines of the Th1 type (IFN gamma, IL-2) plus IL-1 beta expression in untreated alopecia areata (AA), and a switch to high levels of IL-10 TGF-beta 1 expression after successful treatment with the contact allergen diphenylcyclopropenone (DCP). Hence the question arose as to whether cytokines are able to arrest hair growth and whether IL-10 or TGF beta 1 have the capacity to antagonize this process. Using whole-organ cultures of microdissected human hair follicles we studied the effect of a panel of cytokines and growth factors on hair growth and on the gross morphology of the hair follicles in vitro. IL-2, IL-10 and IFN-gamma had no effect in this regard, whereas TGF beta 1 partially inhibited hair growth and EGF, TNF alpha and IL-1 beta completely abrogated it. EGF and TNF alpha induced the formation of a club-like hair follicle, similar to catagen morphology of the hair bulb, whereas hair follicles grown in the presence of IL-1 beta or TGF beta 1 showed no particular morphological changes. We conclude that cytokines and growth factors are pivotal regulators of hair growth at least in vitro. IL-1 is suggested as playing an important role during the pathogenesis of AA. Possible mediators of therapeutic contact dermatitis (IL-10, TGF beta 1, TNF alpha, PGE2) are, at least in vitro, not able to antagonize the IL-1 beta-triggered hair growth inhibition. Therefore, we infer that these mediators rather "modulate' the immune response in AA.

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Cultured Human Hair Follicles and Growth Factors

Abstract: 2 weeks, conditions that favor proliferation of keratinocytes but not of mesenchymal cells. Finally, combining LF24 cells with HF buds and FDCs yielded enlarged,. densely haired grafts that were also

Cited by 30 publications

References 3 publications

Pro-Inflammatory Cytokine Cascade in Human Plucked Hair

Pro-Inflammatory Cytokine Cascade in Human Plucked Hair

Androgenic alopecia and dutasteride in hair mesotherapy: A short review

Cytokines and growth factors influence hair growth in vitro. Possible implications for the pathogenesis and treatment of alopecia areata

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