Culture of diploid and heteroploid cells on dextran “microbeads” in suspension: Studies of growth kinetics

Abstract

of MIT, who very kindly supplied the microbeads on loan.

Growth of an adherent continuous cell line entrapped in a peg/alginate matrix

“…Thus, the procedure of bead re-solubilization, dilution and reformation apparently had no detrimental effect on cell growth. The cell densities achieved in the PEG/alginate beads are compared to those obtained with other microcarriers in Figure 2 (Baijot et al 1987;Beaudry et al, 1979;Butler, 1985Butler, , 1987Clark, 1981;Crespi, 1981;Forestall et al, 1992;Giard et al, 1977Giard et al, , 1979Himes & Hu, 1987;Hu et al, 1987;Levine et al, 1978Matsushita et al, 1990Matsushita et al, , 1991Reuveny et al, 1983;Runstadler & Cernek, 1988;Scattergood et al, 1983;Tat et al, 1988;Thill~'.. et al, 1982;van Hemert et al, 1969;Varani et al, 1983;Whiteside & Spier, 1981;Whiteside et al, 1982;Yanagi et al, 1992). When corrected for microcarrier loading in the reactor, the capacity of the PEG/alginate beads, as might be expected, is comparable to that demonstrated for other macroporous carriers, such as the Verax collagen beads (Runstadler, 1988).…”