Microcarriers in Cell Culture: Structure and Applications

Reuveny, Shaul

doi:10.1016/b978-0-12-007904-9.50014-9

Cited by 15 publications

(2 citation statements)

References 91 publications

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“…The reason is obvious that their porosity increases dramatically the surface area for cell attachment. The surface area of Cytopore is 2.8 m 2 g −1 , while that of Cytodex and Biosilon is only 6000 cm 2 g −1 and 225 cm 2 g −1 , respectively (Reuveny, 1985). 2) As the majority of cells grow inside the pores, they are protected against damage from shearing stress.…”

Section: Discussionmentioning

confidence: 97%

Untitled

Xiao¹,

Huang²,

Li³

et al. 1999

Cytotechnology

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Using porous microcarrier Cytopore and a low-serum medium supplement BIGBEF-3, we have successfully cultivated recombinant CHO cell line CL-11G producing prourokinase and hybridomas producing anti-prourokinase monoclonal antibody in Celligen 1.5 or 5 L bioreactor. The cell density obtained ranged from 1 to 2 x 107 cells mL-1. The yields of prourokinase and monoclonal antibody increased with increasing cell density. As the cells could spontaneously release from and reattach to porous microcarriers, it was very easy to scale-up the cultivation. Thus the bead to bead cell transfer method has been used to scale up the cultivation of CL-11G cells to a 20 L reactor-scale for the pilot production of prourokinase, and also to scale-up the culture of hybridomas for the production of monoclonal antibody for the purification of prourokinase.

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Section: Discussionmentioning

confidence: 97%

Untitled

Xiao¹,

Huang²,

Li³

et al. 1999

Cytotechnology

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“…There have been extensive studies on immobilization of animal cells and its application in large-scale production of mammalian cells and associated products, such as hybridoma cells in the production of monoclonal antibodies (Reuveny, 1985;Heath and Belfort, 1987). The development of immobilized insect * To whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Insect Cell Culture: Fundamental and Applied Aspects

Vlak¹,

Gooijer²,

Tramper³

et al. 2002

Current Applications of Cell Culture Engineering

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Fermentation

Junker

2004

Kirk-Othmer Encyclopedia of Chemical Technology

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Fermentation processes, products, and economic aspects are summarized according to the product classes of primary metabolites, secondary metabolites, biomass, enzymes (and whole cell biocatalysts), and biologicals. Although the information is focused on aerobic stirred tank processes, anaerobic, bubble column, and/or immobilized production techniques are referenced where applicable. Advantages of biochemical production over strictly synthetic processes are discussed. The history of fermentation is described at time periods of pre‐1700s, 1700s–1900, 1900–1945, 1945–1960, 1960–1970, 1970–1995, and 1995 to the present. This progression is followed by an overview of microbiological and fermentation principles, equipment requirements, inoculum development and scale‐up techniques, utilities, and regulatory considerations. Throughout this article specific product and process examples are included.

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Microcarriers in Cell Culture: Structure and Applications

Cited by 15 publications

References 91 publications

Untitled

Untitled

Insect Cell Culture: Fundamental and Applied Aspects

Fermentation

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