Growth of an adherent continuous cell line entrapped in a peg/alginate matrix

Gates, Gary J.; Phillips, Janice

doi:10.1007/bf02447824

Cited by 1 publication

(1 citation statement)

References 39 publications

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“…The incorporation of PEG within alginate hydrogels for three-dimensional cell culture or drug delivery is well-established; , however, the use of these hydrogels in cell-affinity chromatography is relatively new. This paper demonstrates how four-arm, amine-terminated PEG molecules can not only increase the purity of captured EPCs by suppression of nonspecific binding but also enhance capture yield by providing more tether points for capture antibodies.…”

Section: Introductionmentioning

confidence: 99%

Engineered Alginate Hydrogels for Effective Microfluidic Capture and Release of Endothelial Progenitor Cells from Whole Blood

2011

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Microfluidic adhesion-based cell separation systems are of interest in clinical and biological applications where small sample volumes must be processed efficiently and rapidly. While the ability to capture rare cells from complex suspensions such as blood using microfluidic systems has been demonstrated, few methods exist for rapid and non-destructive release of the bound cells. Such detachment is critical for applications in tissue engineering and cell-based therapeutics in contrast with diagnostics wherein immunohistochemical, proteomic, and genomic analyses can be carried out by simply lysing captured cells. This paper demonstrates how the incorporation of 4-arm amine terminated poly(ethylene glycol) (PEG) molecules along with antibodies within alginate hydrogels can enhance the ability of the hydrogels to capture endothelial progenitor cells (EPCs) from whole human blood. The hydrogel coatings are applied conformally onto pillar structures within microfluidic channels and their dissolution with a chelator allows for effective recovery of EPCs following capture.

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Section: Introductionmentioning

confidence: 99%