Culture media for the isolation of campylobacters

Corry, Janet E L; Post, D.E.; Colin, P.; Laisney, Marie-José

doi:10.1016/0168-1605(95)00044-k

Cited by 250 publications

(192 citation statements)

References 98 publications

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“…Campylobacter spp. can be isolated from stool specimens on selective media containing antibiotics, which, in combination with a high temperature of incubation (42-43 °C), inhibits the growth of the physiological bacterial fl ora [3]. Cultural isolation, however, may fail since Campylobacter spp.…”

Section: Introductionmentioning

confidence: 99%

Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Regnath¹,

Ignatius²

2014

European Journal of Microbiology and Immunology

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Campylobacter spp. are fastidious microorganisms, and their detection by culture depends on the freshness of the stool sample and the skills of the laboratory staff. To improve laboratory diagnosis, assays for the detection of specific antigens have been developed. Here, we evaluated two assays for the detection of Campylobacter spp.-specific antigens, i.e., one immunochromatographic test and one enzyme-linked immunosorbent assay (EIA), in 38 frozen Campylobacter spp.-positive specimens and prospectively in 533 fresh stool samples with a conventional enzyme immunoassay (EIA) and culture.Both assays were positive for 36 samples with Campylobacter jejuni and one with Campylobacter coli among 38 Campylobacter spp.-positive frozen samples. One Campylobacter lari-positive sample was identified by the immunochromatographic assay (ICA) only. In a prospective study performed within the course of routine microbiology, both assays were positive for 24/25 C. jejuni culture-positive samples (positive percent agreement, 96.0% [95% CI: 78.9-100%]). ICA and EIA also were positive for 14 and 10 culture-negative samples, respectively (negative percent agreement: ICA, 97.2% [95% CI: 95.4-98.4%]; EIA, 98.0% [95% CI: 96.4-99.0%]). In conclusion, the high agreement between both antigen-detection assays and culture indicates that both assays may be initially performed followed by culture only upon a positive test result.

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Section: Introductionmentioning

confidence: 99%

Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Regnath¹,

Ignatius²

2014

European Journal of Microbiology and Immunology

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“…They are oxidase positive with at least one polar flagellum at one or both ends, giving a corkscrew or darting motility. Campylobacters and helicobacters are microaerophilic, whereas arcobacters may also grow aerobically (Bolton et al 1992;Vandamme and Goosens 1992;Ursing et al 1994;Corry et al 1995). Besides using traditional phenotypic tests, these three genera can be distinguished by whole cell protein profiling Yanglong et al 1991;Costas et al 1993) and also by examination of their 16 s rRNA, cellular fatty acid content and G ¦ C content of their DNA Paster et alWithin these genera are known human pathogens, capable of causing gastroenteritis and other diseases such as bacteraemia, arthritis and Reiters syndrome (Kapperud and Rosef 1983;Skirrow 1990;Park et al 1991;Jacobs Reitsma et al 1994;Skirrow 1994;.…”

Section: Introductionmentioning

confidence: 99%

“…jejuni, C. coli and C. lari) which have been investigated most (Fricker and Park 1989;Corry et al 1995;Atabay et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of campylobacters and arcobacters in ducks at the abattoir

Ridsdale

Atabay

Corry

1998

Journal of Applied Microbiology

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Ten duck carcasses, five from each of two different flocks, and four pairs of pooled duck caecal contents, each pair from a separate flock, were examined by a variety of techniques for arcobacters and campylobacters. Campylobacter coli, C. jejuni ssp. jejuni, C. upsaliensis, Arcobacter cryaerophilus and A. butzleri were isolated from duck caecal contents. Campylobacter coli, C. jejuni ssp. jejuni, A. cryaerophilus, A. butzleri and A. skirrowii were isolated from carcasses. The most effective methods for isolating these bacteria from carcasses involved selective enrichment in campylobacter enrichment broth, containing a cefoperazone, amphotericin, teicoplanin supplement, followed by plating onto modified charcoal cefoperazone deoxycholate agar (mCCDA), or plating onto non‐selective blood agar after filtration through a 0·65 μm pore size cellulose acetate filter. In contrast, recovery from caecal contents was most effective by direct plating onto mCCDA. API test strips performed poorly, failing to identify A. skirrowii or A. butzleri (which are not included in the scheme), or even many common campylobacters. The Preston biochemical characterization scheme was more helpful, though it did not distinguish between Arcobacter species. The species of most isolates of campylobacter, identified using the Preston scheme, was confirmed by the use of SDS‐PAGE of whole cell proteins and this technique was also used successfully to speciate arcobacters.

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“…Consequently, it is essential to have data on the sensitivity and specificity of tests to detect an infection at flock level. Generally flocks are tested for the presence of Campylobacter by culturing fecal and/or cecal samples (Corry et al, 1995;Musgrove et al, 2001;Payne et al, 1999;Berndston et al, 1996). Fecal samples are easier to gather, but it is generally assumed that cecal samples are more appropriate, since Campylobacter mainly colonizes the cecum and, consequently, fecal samples often contain a lower number of bacteria per gram than cecal samples (Rudi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The sensitivity and specificity of fecal and cecal culture for the detection of Campylobacter in Dutch broiler flocks quantified by Bayesian analysis

Woldemariam

Bouma

Vernooij

et al. 2008

International Journal of Food Microbiology

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Dutch broiler flocks are routinely tested for the presence of thermotolerant Campylobacter spp. using a standard cultural procedure for fecal and cecal samples. The objective of this study was to estimate the sensitivity and specificity of fecal and cecal culture for detection of Campylobacter colonization in broiler flocks in absence of a gold standard. Data from 1600 flocks were used from two different populations, whereby only flocks with both fecal and cecal culture results were included in the analysis. Latent class analysis using Bayesian inference was applied to generate the test characteristics of fecal and cecal culture. Two statistical models assuming conditional dependence of both tests on Campylobacter status were used to compare the results. On flock level, the sensitivity of the fecal culture was found to be 21% (95% CI: 12, 31) and 23% (95% CI: 13, 60), and the specificity was 98% (95% CI: 94, 99) and 97% (95% CI: 92, 99) for the two models, respectively. The sensitivity of the cecal culture was 64% (95% CI: 37, 89) and 66% (95% CI: 39, 90), and the specificity was 98% (95 CI: 94, 99) and 95% (95% CI: 72, 99) in respective models. The implications of a low sensitivity as in the case of the fecal culture is important for the design and interpretation of monitoring programmes and may result in excessive false negative test results. Although cecal culture is the more sensitive test, substantial misclassification of infected flocks may still occur.

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Culture media for the isolation of campylobacters

Cited by 250 publications

References 98 publications

Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Accurate detection ofCampylobacterspp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory

Prevalence of campylobacters and arcobacters in ducks at the abattoir

The sensitivity and specificity of fecal and cecal culture for the detection of Campylobacter in Dutch broiler flocks quantified by Bayesian analysis

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