Cultivation of shiitake mushroom, Lentinula edodes, in several lignocellulosic materials originating from the subtropics

Salmones, Dulce; Mata, Gerardo; Ramos, Luz Ma.; Waliszewski, Krzystof N.

doi:10.1051/agro:19990102

Cited by 39 publications

(28 citation statements)

References 5 publications

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“…A higher number of points of inoculum help in a better colonization of the substrate. Actually, if the grain is small, the farmer will have a higher number of points of inoculum by weight of spawn and as a result, a higher number of colonies and a faster colonization of the substrate (Salmones et al, 1999). On the other hand, with the use of beads, although the number of points of inoculum is higher than with the use of grains, we do not observe a higher effect on the percentage of colonization.…”

Section: Substrate Colonizationcontrasting

confidence: 66%

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Artificial spawn generation based on alginate encapsulated mycelium as inoculum for mushroom cultivation

Ortiz¹,

Colavolpe²,

Albertó³

2017

Afr. J. Biotechnol.

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Spawn quality is an important factor that affects the final yield in mushroom production. One of the most common risks is the contamination of substrates and spawn by mitosporic fungi. Some species do not easily grow on grain substrate as traditional species do and these find it real difficult to produce spawn. A method was evaluated to produce spawn for mushroom production based on aseptically encapsulated mycelium using alginate beads. Strains of four selected edible mushrooms were cultivated in liquid medium, mycelium was harvested by filtration. Subsequently, it was blended with 100 mL of 2% (w/v) sodium alginate solution or with the alginate solution with the addition of 2% (w/v) yeast extract solution or with the addition of 2% (w/v) meat extract solution. An electronic mixer was used to disaggregate the mycelium and generate a homogeneous suspension. To obtain the artificial spawn, the cells suspensions were dripped in 200 mL of 500 mM CaCl 2 solution using a cut micro-tip. Beads obtained were inoculated in potato dextrose agar Petri dishes and in substrate (wheat straw or Poplar sawdust) to evaluate the growth. Tests carried out using this method, demonstrated that the incorporation of a suitable nutrient increases the ability of selected mushroom to emerge from the beads. Mycelia colonization tests were carried out on sterilized substrate, one using alginate beads and another one using the traditional spawn as inoculum. No significant differences according to t-test (p<0.05) were shown. However, spawning production based on alginate beads can be performed in only a few days, whereas the traditional method requires more time. The method proposed here represents an antecedent to improve spawn generation for future industrial applications, for further studies and for other uses as mycelium inoculant.

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Section: Substrate Colonizationcontrasting

confidence: 66%

“…The spawn quality is an important factor that affects the final yield in the mushroom production. At present, a variety of different substrates such as: manure, tobacco stems and wheat, rye or millet grains have been successfully used in the spawn preparation (Salmones et al, 1999;San Antonio, 2011).…”

Section: Introductionmentioning

confidence: 99%

Artificial spawn generation based on alginate encapsulated mycelium as inoculum for mushroom cultivation

Ortiz¹,

Colavolpe²,

Albertó³

2017

Afr. J. Biotechnol.

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“…Also, this substrate is very susceptible to drying, which affected sporophore formation [8]. Given the physical nature and high porosity of the rice husk, and also the fact that it dries up very fast, it will be advisable to use it as an additive to sawdust for use as mushroom substrate.…”

Section: Discussionmentioning

confidence: 99%

Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products

Obodai¹,

Cleland-Okine²,

Vowotor³

2003

J IND MICROBIOL BIOTECHNOL

176

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Eight lignocellulosic by-products were evaluated as substrates for cultivation of the oyster mushroom, Pleurotus ostreatus ( Jacq. ex. fr) Kummer. The yields of mushroom on the different substrates were 183.1, 151.8, 111.5, 87.8, 49.5, 23.3, 13.0 and 0.0 g for composted sawdust of Triplochiton scleroxylon, rice straw, banana leaves, maize stover, corn husk, rice husk, fresh sawdust, and elephant grass, respectively. The biological efficiency (BE) followed the same pattern and ranged from 61.0% for composted sawdust to 0.0% for elephant grass. The yield of mushroom was positively correlated to cellulose ( r(2) =0.6), lignin ( r(2) =0.7) and fibre ( r(2) =0.7) contents of the substrates. Based on the yield and BE of the substrates tested, rice straw appeared to be the best alternate substrate for growing oyster mushrooms.

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“…for the strain C-Agro1 (55%), (1994) for strain IE-105 (43.0 %) and IE-40 (52.3%) obtained in coffee pulp, but lower than those reported by Salmones et al (1999) in bagasse and leaves of sugarcane, which dominated the G2 (57 and 63%, respectively) and in the bracts of pineapple crown predominated G3 (45%) similar to the formula C-Agro1 ( 40%). Rural conditions of the experimental plant did not affect the TP, TB and EB of the CP-CA1 as quality fruiting bodies on a regular basis (85%) were obtained and only 15% is irregular fruiting bodies (Table 3), in the C-Agro2 formula.…”

Section: Agradecimientosmentioning

confidence: 70%

“…Valores similares a los reportados por Bernabé et al (2006) para la cepa IE-40, donde predominó el G2 (60.2%), seguido por la formula C-Agro1 (55%) superiores a lo reportado por Mata y Hernández, (1994) para la cepa IE-105 (43.0%) y IE-40 (52.3%) obtenidos en la pulpa de café, pero inferiores a lo reportado por Salmones et al (1999) en bagazo y en las hojas de caña de azúcar, donde predominó el G2 (57 y 63%, respectivamente) y en las brácteas de la corona de piña predominó el G3 (45%) similares para la formula C-Agro1 (40%). Las condiciones rurales de la planta experimental no afectaron la TP, TB y EB de la CP-CA1, ya que se obtuvieron cuerpos fructíferos de calidad de forma regular (85%) y solo 15% representa cuerpos fructíferos irregulares (Cuadro 3), en la formula C-Agro2.…”

Section: Biological Efficiency Quantification (Eb) Production Time (unclassified

Producción del hongo Shiitake (Lentinula edodes Pegler) en bloques sintéticos utilizando residuos agroforestales

Romero‐Arenas

Guerrero

Huato

et al. 2017

Remexca

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El cultivo de hongos comestibles es una alternativa importante para satisfacer las necesidades alimenticias de la población, desarrollo agrícola y formación de agroindustrias en muchos lugares del mundo. Dentro de los hongos comestibles, el Shiitake posee propiedades alimenticias y medicinales importantes para la industria al utilizar los residuos agrícolas y forestales como sustrato para su producción. El objetivo de este estudio fue evaluar las fórmulas C-Agro1, 2 y 3 en la producción del hongo Shiitake, utilizando la cepa CP-CA1 de Lentinula edodes (Pegler) en bloques sintéticos, conformados por residuos agroforestales de la Sierra Norte del estado de Puebla, como es el aserrín de encino (Quercus sp.), restos de tallos y hojas (rastrojo) así mismo, olote triturado de maíz (Zea mays). La cepa CP-CA1 demostró un adecuado crecimiento miceliar sobre los bloques sintéticos, con una tasa de producción de 0.38, 0.33 y 0.31% para C-Agro1, 2 y 3 respectivamente. La mayor ef iciencia biológica (EB) se obtuvo en la en la formula C-Agro2 a los 265 días de producción, con 100.50%. La presencia de cuerpos fructíferos fue de 85% de forma regular y con un peso aproximado entre 40 a 70 g por unidad. Los resultados demostraron la factibilidad de cultivar la cepa CP-CA1 de Lentinula edodes sobre residuos agroforestales de la sierra norte del estado de Puebla.

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Cultivation of shiitake mushroom, Lentinula edodes, in several lignocellulosic materials originating from the subtropics

Abstract: -Mycelial growth of two strains of the mushroom Lentinula edodes

Cited by 39 publications

References 5 publications

Artificial spawn generation based on alginate encapsulated mycelium as inoculum for mushroom cultivation

Artificial spawn generation based on alginate encapsulated mycelium as inoculum for mushroom cultivation

Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products

Producción del hongo Shiitake (Lentinula edodes Pegler) en bloques sintéticos utilizando residuos agroforestales

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